cDNA Clone TSC22D2

• Synonyms: TILZ4a; TILZ4b; TILZ4c
• Plasmid: pCMV5 FLAG1 TSC22
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG1 TSC22
• Genbank: NM_014779
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGCCGGCCAAGAAGAAGAGCTGCTTCCAGATCACCAGTGTCACCACGGCCCAGGTGGCCACTAGCATCACCGAGGACACCGAGAGCTTGGACGACCCGGACGAGTCACGCACAGAGGACGTCTCTTCCGAGATTTTCGACGTCTCTCGGGCCACGGATTATGGCCCTGAGGAGGTCTGCGAGCGCAGCTCTTCCGAAGAGACGCTTAACAATGTTGGGGATGCGGAGACTCCCGGGACCGTCTCCCCAAACCTCCTCCTAGATGGGCAGCTGGCAGCGGCGGCTGCTGCTCCCGCCAACGGAGGAGGAGTCGTTTCGGCCCGGAGCGTGTCTGGGGCGCTCGCCAGTACCCTGGCGGCGGCTGCCACTTCGGCCCCCGCCCCCGGAGCACCCGGCGGCCCCCAGCTCGCGGGCTCATCCGCCGGGCCAGTGACTGCAGCCCCATCTCAGCCTCCCACCACATGTAGTTCCCGTTTTCGCGTGATCAAGCTGGACCACGGGAGCGGAGAGCCCTATAGACGCGGCCGATGGACGTGTATGGAATACTATGAGAGGGATTCAGACAGCAGCGTCCTGACTAGATCCGGGGATTGCATTAGACACAGCAGTACTTTTGACCAGACTGCGGAGCGGGACAGCGGCCTGGGCGCCACCGGAGGGTCGGTGGTGGTAGTAGTGGCCTCCATGCAGGGGGCGCACGGGCCCGAGTCGGGAACTGACAGCTCCTTGACTGCTGTGTCACAGCTACCCCCGTCGGAGAAAATGAGCCAGCCCACTCCGGCCCAGCCGCAGAGTTTTAGCGTTGGGCAGCCACAGCCGCCGCCGCCACCCGTAGGTGGGGCTGTGGCTCAAAGCTCGGCTCCGCTGCCGCCGTTCCCGGGAGCCGCGACCGGGCCGCAGCCAATGATGGCAGCCGCGCAGCCCAGCCAGCCCCAGGGAGCGGGGCCCGGGGGACAGACTCTGCCGCCGACGAATGTAACCCTGGCGCAGCCGGCTATGTCCCTGCCTCCGCAGCCGGGCCCTGCAGTGGGCGCCCCCGCGGCGCAGCAGCCCCAGCAGTTCGCGTATCCTCAGCCTCAGATACCGCCCGGACATTTGCTGCCCGTCCAGCCCTCCGGCCAGAGTGAGTACCTGCAGCAGCACGTGGCCGGCCTGCAGCCGCCAAGCCCCGCGCAGCCCTCGTCCACCGGCGCCGCAGCGAGCCCCGCCACGGCGGCCACCCTTCCCGTGGGCACCGGCCAGAATGCTTCCTCGGTGGGCGCGCAGCTCATGGGCGCGTCTTCCCAGCCCAGCGAAGCCATGGCCCCCCGGACGGGACCAGCGCAAGGCGGGCAGGTCGCGCCTTGTCAGCCGACTGGAGTGCCCCCGGCTACTGTGGGAGGCGTGGTGCAGCCGTGCCTCGGTCCTGCCGGGGCTGGGCAGCCCCAGTCCGTGCCTCCGCCGCAGATGGGTGGCAGTGGTCCGCTGTCAGCCGTACCTGGTGGCCCTCACGCCGTGGTGCCCGGAGTTCCAAACGTGCCTGCAGCCGTGCCCGCTCCAAGCGTGCCTAGTGTGTCTACCACTTCTGTTACTATGCCAAATGTACCCGCGCCTCTGGCCCAGTCGCAACAGCTGAGCAGCCATACGCCAGTCAGCAGGAGCAGCAGCATAATCCAGCATGTTGGGCTGCCCTTAGCGCCAGGCACACACAGCGCACCAACAAGTCTACCACAGTCTGACCTAAGCCAGTTTCAAACTCAGACCCAGCCTTTAGTCGGGCAAGTCGACGATACTAGAAGAAAATCAGAACCCCTACCTCAACCACCACTTTCTCTCATTGCTGAAAATAAGCCTGTTGTGAAGCCGCCTGTTGCAGATTCCCTGGCAAACCCCCTTCAGTTAACACCTATGAACAGTCTGGCCACCTCTGTATTCAGCATAGCTATTCCTGTTGATGGTGATGAAGACAGGAATCCTTCAACTGCTTTCTACCAAGCGTTCCATTTGAACACGTTAAAGGAATCAAAGAGCCTCTGGGATAGTGCATCTGGGGGAGGTGTTGTAGCCATTGACAACAAAATAGAACAAGCAATGGATCTGGTGAAAAGCCATTTGATGTATGCAGTAAGAGAAGAAGTGGAAGTTTTAAAGGAACAAATAAAAGAATTAGTTGAAAGAAACTCTTTACTTGAACGAGAAAATGCACTGTTAAAATCTCTTTCAAGCAATGATCAATTATCCCAACTCCCAACCCAACAGGCCAATCCTGGTAGCACTTCTCAACAGCAAGCAGTGATAGCACAGCCTCCGCAGCCAACGCAACCTCCACAGCAGCCGAATGTCTCCTCAGCATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMPAKKKSCFQITSVTTAQVATSITEDTESLDDPDESRTEDVSSEIFDVSRATDYGPEEVCERSSSEETLNNVGDAETPGTVSPNLLLDGQLAAAAAAPANGGGVVSARSVSGALASTLAAAATSAPAPGAPGGPQLAGSSAGPVTAAPSQPPTTCSSRFRVIKLDHGSGEPYRRGRWTCMEYYERDSDSSVLTRSGDCIRHSSTFDQTAERDSGLGATGGSVVVVVASMQGAHGPESGTDSSLTAVSQLPPSEKMSQPTPAQPQSFSVGQPQPPPPPVGGAVAQSSAPLPPFPGAATGPQPMMAAAQPSQPQGAGPGGQTLPPTNVTLAQPAMSLPPQPGPAVGAPAAQQPQQFAYPQPQIPPGHLLPVQPSGQSEYLQQHVAGLQPPSPAQPSSTGAAASPATAATLPVGTGQNASSVGAQLMGASSQPSEAMAPRTGPAQGGQVAPCQPTGVPPATVGGVVQPCLGPAGAGQPQSVPPPQMGGSGPLSAVPGGPHAVVPGVPNVPAAVPAPSVPSVSTTSVTMPNVPAPLAQSQQLSSHTPVSRSSSIIQHVGLPLAPGTHSAPTSLPQSDLSQFQTQTQPLVGQVDDTRRKSEPLPQPPLSLIAENKPVVKPPVADSLANPLQLTPMNSLATSVFSIAIPVDGDEDRNPSTAFYQAFHLNTLKESKSLWDSASGGGVVAIDNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELVERNSLLERENALLKSLSSNDQLSQLPTQQANPGSTSQQQAVIAQPPQPTQPPQQPNVSSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 FLAG1 TSC2262.26 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU6784
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has first 3 amino acids MSK missing as compared with the updated reference sequence
• Low Temp: No