cDNA Clone RICTOR (human)

• Plasmid: pEBG-2T-full RICTOR human
• Parent Plasmid: pEBG2T
• Expressed: GST-full RICTOR human
• Genbank: NM_152756 with changes
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACTAGTGGTACCATCGATAAAGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGCGGCGATCGGCCGAGGACGCTCTCTGAAGAACCTCCGAGTACGAGGGCGGAATGACAGCGGCGAGGAGAACGTCCCGCTGGATCTGACCCGAGAACCTTCTGATAACTTAAGAGAGATTCTCCAAAATGTGGCCAGATTGCAGGGAGTATCAAATATGAGAAAGCTAGGCCATCTGAATAACTTTACTAAGCTTCTTTGTGATATTGGCCACAGTGAAGAAAAACTGGGCTTTCACTATGAGGATATCATAATTTGTTTGCGGTTAGCTTTATTAAATGAAGCAAAAGAAGTGCGAGCAGCAGGGCTACGAGCGCTTCGATATCTCATCCAAGACTCCAGTATTCTCCAGAAGGTGCTAAAATTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATACAACAGAGCAACGAGGTAGAGAGGACACAAGCACTTCGATTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTGTTTCCTAGTTCTGTGACCAACTCATTAATTGCAGTTGGAAATGATGGACTTCAAGAAAGAGACAGAATGGTCCGAGCATGCATTGCCATTATCTGTGAACTAGCACTTCAGAATCCAGAGGTGGTGGCCCTTCGAGGTGGACTAAACACCATCTTGAAAAATGTGATCGATTGCCAATTAAGTCGAATAAATGAGGCCCTAATTACTACAATTTTGCACCTTCTTAATCATCCAAAGACTCGACAGTATGTGCGAGCTGATGTAGAATTAGAGAGAATTTTAGCACCCTATACTGATTTTCACTACAGACATAGTCCAGATACAGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGGTTTCTAGCCAGTAAAATGGGAATCATAGCAACATTCCGATCATGGGCAGGTATTATTAATTTATGTAAACCTGGAAATTCTGGGATCCAGTCTCTAATAGGAGTACTTTGCATACCAAATATGGAAATAAGGCGAGGTCTACTTGAAGTGCTTTATGATATATTTCGTCTTCCTCTACCTGTTGTGACTGAGGAGTTCATAGAAGCACTACTCAGTGTAGATCCAGGGAGGTTCCAAGACAGTTGGAGGCTTTCAGATGGCTTTGTGGCAGCTGAGGCAAAAACTATTCTTCCTCATCGTGCCAGATCCAGGCCAGACCTCATGGATAATTATTTGGCACTGATACTCTCTGCATTTATTCGTAATGGACTTTTAGAGGGTCTAGTTGAAGTGATAACAAACAGTGATGATCATATCTCAGTTAGAGCTACCATCCTTTTAGGAGAGCTTTTACATATGGCAAACACAATTCTTCCTCATTCACATAGCCATCATTTACACTGCTTGCCAACCCTAATGAATATGGCTGCATCCTTTGATATCCCCAAGGAAAAGAGACTGCGAGCCAGTGCAGCCTTGAACTGTTTAAAACGCTTCCATGAAATGAAGAAACGAGGACCTAAGCCTTATAGTCTTCATTTAGACCACATTATTCAGAAAGCAATTGCAACACACCAGAAACGGGATCAGTATCTCCGAGTTCAGAAAGATATATTTATCCTTAAGGATACAGAGGAAGCTCTTTTAATTAACCTTAGAGATAGCCAAGTCCTTCAACATAAAGAGAATCTTGGATGGAATTGGAATCTTATAGGGACCATTCTTAAGTGGCCAAATGTAAATCTAAGAAACTATAAAGATGAACAGTTACACAGGTTTGTACGAAGACTACTTTATTTTTACAAGCCCAGCAGTAAATTATATGCCAACCTGGATCTGGATTTTGCCAAGGCCAAACAGCTCACGGTTGTAGGTTGCCAGTTTACAGAATTTCTTCTTGAATCTGAAGAGGATGGGCAAGGCTACTTAGAAGATCTAGTAAAGGATATTGTTCAGTGGCTCAATGCTTCATCTGGAATGAAACCCGAAAGAAGTCTTCAAAATAATGGTTTATTGACCACCCTTAGTCAACACTACTTTTTATTTATTGGAACACTTTCTTGCCACCCTCATGGAGTTAAAATGCTGGAAAAATGCAGTGTATTTCAGTGTCTCCTTAATCTTTGCTCCTTGAAAAACCAAGATCACTTGCTAAAACTTACTGTTTCTAGCTTGGACTATAGCAGAGATGGATTGGCTAGAGTCATCCTTTCCAAAATTTTAACTGCAGCTACTGATGCCTGCAGACTCTATGCAACAAAACATTTAAGGGTATTATTGAGAGCTAATGTTGAATTCTTTAATAATTGGGGAATTGAGTTGTTAGTGACCCAGCTACATGATAAAAACAAAACGATTTCCTCTGAAGCTCTTGATATCCTCGATGAAGCATGTGAAGACAAGGCCAATCTTCATGCTCTCATTCAGATGAAACCAGCGTTATCCCACCTTGGAGACAAGGGTTTGCTTCTCCTGCTGAGATTTCTCTCCATTCCAAAAGGACTTTCCTATCTGAATGAAAGAGGTTATGTAGCAAAACAATTGGAAAAGTGGCACAGGGAATACAACTTCAAATATGTTGACTTGATTGAGGAACAACTCAATGAAGCACTTACTACTTACCGGAAGCCTGTTGATGGTGATAACTATGTTCGTCGGAGTAACCAAAGATTACAGCGTCCTCACGTCTACCTGCCTATACACCTTTATGGACAACTAGTACACCATAAAACAGGCTGCCATTTGTTGGAAGTACAGAATATTATTACAGAACTCTGTCGTAATGTTCGTACACCAGATTTGGATAAGTGGGAAGAAATTAAAAAACTGAAAGCATCTCTTTGGGCCTTGGGAAATATCGGCTCATCAAATTGGGGTCTCAATTTGCTACAGGAAGAAAACGTGATTCCAGATATACTAAAACTTGCAAAACAGTGTGAAGTTCTTTCCATCAGAGGGACCTGTGTATATGTACTTGGGCTCATAGCTAAAACCAAACAAGGCTGTGATATTCTAAAATGTCACAACTGGGATGCTGTGAGGCATAGTCGCAAACATCTGTGGCCAGTGGTTCCAGATGATGTGGAACAACTCTGTAATGAACTTTCATCTATCCCAAGCACTCTAAGTTTGAACTCGGAGTCAACCAGCTCTAGACATAATAGTGAAAGTGAATCTGTGCCATCGAGTATGTTCATATTGGAGGATGACCGGTTTGGCAGCAGCTCTACTAGTACATTTTTCCTTGATATCAATGAAGATACAGAGCCAACATTTTATGACCGATCTGGACCCATAAAGGATAAAAATTCATTCCCTTTCTTTGCTTCTAGTAAACTTGTGAAGAATCGTATCTTAAATTCGCTTACTTTGCCTAACAAAAAACATCGTAGTAGCAGTGATCCAAAAGGAGGGAAATTATCATCTGAAAGTAAGACAAGCAACAGGCGAATCAGAACACTTACGGAGCCCAGTGTTGATTTTAATCATAGTGATGATTTTACACCCATATCCACTGTACAGAAAACATTACAATTAGAGACTTCATTTATGGGGAATAAGCACATTGAAGACACTGGTAGTACACCAAGCATTGGAGAAAATGACTTAAAATTCACCAAGAATTTTGGTACAGAGAATCACAGAGAAAATACAAGCCGAGAGAGGTTAGTAGTAGAAAGTTCAACGAGCTCACATATGAAGATACGTAGCCAAGGTTTCAATACAGACACTACAACAAGTGGCATAAGTTCAATGAGCTCAAGTCCTTCACGAGAGACAGTAGGTGTAGATGCTACAACTATGGACACAGACTGTGGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTATTTGACGCCACAGTCTAACCATCTGTCTCTCTCCAAATCAAATTCGGTGTCCCTGGTGCCTCCAGGTTCTTCTCATACGCTTCCTAGAAGAGCACAGTCCCTTAAAGCACCCTCTATTGCTACAATTAAAAGTCTAGCAGATTGTAACTTTAGTTACACAAGTTCTAGAGATGCTTTTGGCTATGCTACACTGAAAAGACTACAGCAACAAAGAATGCATCCATCCTTATCTCACTCTGAAGCTTTGGCATCTCCAGCAAAAGATGTGCTATTTACTGATACCATCACCATGAAGGCCAACAGTTTTGAGTCCAGATTAACACCAAGCAGGTTCATGAAAGCCTTAAGTTATGCATCATTAGATAAAGAAGATTTATTGAGTCCTATTAATCAAAATACCCTGCAACGATCTTCCTCAGTGCGGTCCATGGTGTCCAGTGCCACATATGGGGGTTCAGATGATTACATTGGTCTTGCTCTCCCGGTGGATATAAATGATATATTCCAGGTAAAGGATATTCCCTATTTTCAGACAAAAAACATACCACCACATGATGATCGAGGTGCAAGAGCATTTGCCCATGATGCAGGAGGTCTTCCATCTGGAACTGGAGGTCTTGTAAAAAATTCTTTTCACTTGCTACGACAGCAGATGAGTCTTACGGAAATAATGAATTCAATCCATTCAGATGCCTCTCTGTTTTTAGAAAGTACAGAAGACACTGGACTACAGGAACATACAGATGATAACTGCCTTTATTGTGTCTGTATTGAAATTCTGGGTTTCCAGCCCAGCAACCAACTGAGTGCAATATGTAGTCATTCAGACTTTCAAGATATTCCATATTCTGATTGGTGTGAGCAGACTATCCATAATCCTTTAGAAGTGGTTCCCTCTAAGTTTTCGGGGATTTCTGGATGCAGTGATGGGGTGTCTCAAGAAGGCTCAGCTAGCAGCACCAAAAGCACAGAATTGTTACTAGGTGTTAAAACAATTCCAGATGATACACCAATGTGCCGTATACTCCTTCGCAAAGAAGTTCTAAGATTAGTCATTAATTTGAGTAGTTCAGTTTCAACTAAATGTCATGAGACTGGGCTTTTAACAATTAAGGAGAAGTATCCTCAAACATTTGATGACATATGCCTTTACTCTGAGGTTTCCCATTTGCTGTCACACTGCACATTCAGACTTCCGTGTCGGAGGTTCATACAAGAATTATTTCAAGATGTACAGTTTCTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAACACCACCAAAGCGACCTATAGTTGATACATCTGCTGAATCCTGACCGCGGCCGCGACTCTAGAGGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDKATMYPYDVPDYAMAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLGWNWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGLSYLNERGYVAKQLEKWHREYNFKYVDLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSRERLVVESSTSSHMKIRSQGFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPSGTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKRPIVDTSAES

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU6634
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: There is no NCBI Acc. that would be identical with our sequence , however all of the splicing variations exist either in some other accession number or in some EST; none of them are PCR generated mutations.; ; Changes compared to NM_152756; E545 IS G; F815 IS L; S837 IS F; S1219 IS G; Q1699 IS R