cDNA Clone RICTOR (human)

• Plasmid: pCMV5-HA RICTOR human
• Parent Plasmid: pCMV5
• Expressed: HA-RICTOR-human
• Genbank: NM_152756 with changes
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCCTTATAAATCGATAAAGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGCGGCGATCGGCCGAGGACGCTCTCTGAAGAACCTCCGAGTACGAGGGCGGAATGACAGCGGCGAGGAGAACGTCCCGCTGGATCTGACCCGAGAACCTTCTGATAACTTAAGAGAGATTCTCCAAAATGTGGCCAGATTGCAGGGAGTATCAAATATGAGAAAGCTAGGCCATCTGAATAACTTTACTAAGCTTCTTTGTGATATTGGCCACAGTGAAGAAAAACTGGGCTTTCACTATGAGGATATCATAATTTGTTTGCGGTTAGCTTTATTAAATGAAGCAAAAGAAGTGCGAGCAGCAGGGCTACGAGCGCTTCGATATCTCATCCAAGACTCCAGTATTCTCCAGAAGGTGCTAAAATTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATACAACAGAGCAACGAGGTAGAGAGGACACAAGCACTTCGATTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTGTTTCCTAGTTCTGTGACCAACTCATTAATTGCAGTTGGAAATGATGGACTTCAAGAAAGAGACAGAATGGTCCGAGCATGCATTGCCATTATCTGTGAACTAGCACTTCAGAATCCAGAGGTGGTGGCCCTTCGAGGTGGACTAAACACCATCTTGAAAAATGTGATCGATTGCCAATTAAGTCGAATAAATGAGGCCCTAATTACTACAATTTTGCACCTTCTTAATCATCCAAAGACTCGACAGTATGTGCGAGCTGATGTAGAATTAGAGAGAATTTTAGCACCCTATACTGATTTTCACTACAGACATAGTCCAGATACAGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGGTTTCTAGCCAGTAAAATGGGAATCATAGCAACATTCCGATCATGGGCAGGTATTATTAATTTATGTAAACCTGGAAATTCTGGGATCCAGTCTCTAATAGGAGTACTTTGCATACCAAATATGGAAATAAGGCGAGGTCTACTTGAAGTGCTTTATGATATATTTCGTCTTCCTCTACCTGTTGTGACTGAGGAGTTCATAGAAGCACTACTCAGTGTAGATCCAGGGAGGTTCCAAGACAGTTGGAGGCTTTCAGATGGCTTTGTGGCAGCTGAGGCAAAAACTATTCTTCCTCATCGTGCCAGATCCAGGCCAGACCTCATGGATAATTATTTGGCACTGATACTCTCTGCATTTATTCGTAATGGACTTTTAGAGGGTCTAGTTGAAGTGATAACAAACAGTGATGATCATATCTCAGTTAGAGCTACCATCCTTTTAGGAGAGCTTTTACATATGGCAAACACAATTCTTCCTCATTCACATAGCCATCATTTACACTGCTTGCCAACCCTAATGAATATGGCTGCATCCTTTGATATCCCCAAGGAAAAGAGACTGCGAGCCAGTGCAGCCTTGAACTGTTTAAAACGCTTCCATGAAATGAAGAAACGAGGACCTAAGCCTTATAGTCTTCATTTAGACCACATTATTCAGAAAGCAATTGCAACACACCAGAAACGGGATCAGTATCTCCGAGTTCAGAAAGATATATTTATCCTTAAGGATACAGAGGAAGCTCTTTTAATTAACCTTAGAGATAGCCAAGTCCTTCAACATAAAGAGAATCTTGGATGGAATTGGAATCTTATAGGGACCATTCTTAAGTGGCCAAATGTAAATCTAAGAAACTATAAAGATGAACAGTTACACAGGTTTGTACGAAGACTACTTTATTTTTACAAGCCCAGCAGTAAATTATATGCCAACCTGGATCTGGATTTTGCCAAGGCCAAACAGCTCACGGTTGTAGGTTGCCAGTTTACAGAATTTCTTCTTGAATCTGAAGAGGATGGGCAAGGCTACTTAGAAGATCTAGTAAAGGATATTGTTCAGTGGCTCAATGCTTCATCTGGAATGAAACCCGAAAGAAGTCTTCAAAATAATGGTTTATTGACCACCCTTAGTCAACACTACTTTTTATTTATTGGAACACTTTCTTGCCACCCTCATGGAGTTAAAATGCTGGAAAAATGCAGTGTATTTCAGTGTCTCCTTAATCTTTGCTCCTTGAAAAACCAAGATCACTTGCTAAAACTTACTGTTTCTAGCTTGGACTATAGCAGAGATGGATTGGCTAGAGTCATCCTTTCCAAAATTTTAACTGCAGCTACTGATGCCTGCAGACTCTATGCAACAAAACATTTAAGGGTATTATTGAGAGCTAATGTTGAATTCTTTAATAATTGGGGAATTGAGTTGTTAGTGACCCAGCTACATGATAAAAACAAAACGATTTCCTCTGAAGCTCTTGATATCCTCGATGAAGCATGTGAAGACAAGGCCAATCTTCATGCTCTCATTCAGATGAAACCAGCGTTATCCCACCTTGGAGACAAGGGTTTGCTTCTCCTGCTGAGATTTCTCTCCATTCCAAAAGGACTTTCCTATCTGAATGAAAGAGGTTATGTAGCAAAACAATTGGAAAAGTGGCACAGGGAATACAACTTCAAATATGTTGACTTGATTGAGGAACAACTCAATGAAGCACTTACTACTTACCGGAAGCCTGTTGATGGTGATAACTATGTTCGTCGGAGTAACCAAAGATTACAGCGTCCTCACGTCTACCTGCCTATACACCTTTATGGACAACTAGTACACCATAAAACAGGCTGCCATTTGTTGGAAGTACAGAATATTATTACAGAACTCTGTCGTAATGTTCGTACACCAGATTTGGATAAGTGGGAAGAAATTAAAAAACTGAAAGCATCTCTTTGGGCCTTGGGAAATATCGGCTCATCAAATTGGGGTCTCAATTTGCTACAGGAAGAAAACGTGATTCCAGATATACTAAAACTTGCAAAACAGTGTGAAGTTCTTTCCATCAGAGGGACCTGTGTATATGTACTTGGGCTCATAGCTAAAACCAAACAAGGCTGTGATATTCTAAAATGTCACAACTGGGATGCTGTGAGGCATAGTCGCAAACATCTGTGGCCAGTGGTTCCAGATGATGTGGAACAACTCTGTAATGAACTTTCATCTATCCCAAGCACTCTAAGTTTGAACTCGGAGTCAACCAGCTCTAGACATAATAGTGAAAGTGAATCTGTGCCATCGAGTATGTTCATATTGGAGGATGACCGGTTTGGCAGCAGCTCTACTAGTACATTTTTCCTTGATATCAATGAAGATACAGAGCCAACATTTTATGACCGATCTGGACCCATAAAGGATAAAAATTCATTCCCTTTCTTTGCTTCTAGTAAACTTGTGAAGAATCGTATCTTAAATTCGCTTACTTTGCCTAACAAAAAACATCGTAGTAGCAGTGATCCAAAAGGAGGGAAATTATCATCTGAAAGTAAGACAAGCAACAGGCGAATCAGAACACTTACGGAGCCCAGTGTTGATTTTAATCATAGTGATGATTTTACACCCATATCCACTGTACAGAAAACATTACAATTAGAGACTTCATTTATGGGGAATAAGCACATTGAAGACACTGGTAGTACACCAAGCATTGGAGAAAATGACTTAAAATTCACCAAGAATTTTGGTACAGAGAATCACAGAGAAAATACAAGCCGAGAGAGGTTAGTAGTAGAAAGTTCAACGAGCTCACATATGAAGATACGTAGCCAAGGTTTCAATACAGACACTACAACAAGTGGCATAAGTTCAATGAGCTCAAGTCCTTCACGAGAGACAGTAGGTGTAGATGCTACAACTATGGACACAGACTGTGGAAGCATGAGTACTGTGGTAAGTACTAAAACTATTAAGACAAGCCACTATTTGACGCCACAGTCTAACCATCTGTCTCTCTCCAAATCAAATTCGGTGTCCCTGGTGCCTCCAGGTTCTTCTCATACGCTTCCTAGAAGAGCACAGTCCCTTAAAGCACCCTCTATTGCTACAATTAAAAGTCTAGCAGATTGTAACTTTAGTTACACAAGTTCTAGAGATGCTTTTGGCTATGCTACACTGAAAAGACTACAGCAACAAAGAATGCATCCATCCTTATCTCACTCTGAAGCTTTGGCATCTCCAGCAAAAGATGTGCTATTTACTGATACCATCACCATGAAGGCCAACAGTTTTGAGTCCAGATTAACACCAAGCAGGTTCATGAAAGCCTTAAGTTATGCATCATTAGATAAAGAAGATTTATTGAGTCCTATTAATCAAAATACCCTGCAACGATCTTCCTCAGTGCGGTCCATGGTGTCCAGTGCCACATATGGGGGTTCAGATGATTACATTGGTCTTGCTCTCCCGGTGGATATAAATGATATATTCCAGGTAAAGGATATTCCCTATTTTCAGACAAAAAACATACCACCACATGATGATCGAGGTGCAAGAGCATTTGCCCATGATGCAGGAGGTCTTCCATCTGGAACTGGAGGTCTTGTAAAAAATTCTTTTCACTTGCTACGACAGCAGATGAGTCTTACGGAAATAATGAATTCAATCCATTCAGATGCCTCTCTGTTTTTAGAAAGTACAGAAGACACTGGACTACAGGAACATACAGATGATAACTGCCTTTATTGTGTCTGTATTGAAATTCTGGGTTTCCAGCCCAGCAACCAACTGAGTGCAATATGTAGTCATTCAGACTTTCAAGATATTCCATATTCTGATTGGTGTGAGCAGACTATCCATAATCCTTTAGAAGTGGTTCCCTCTAAGTTTTCGGGGATTTCTGGATGCAGTGATGGGGTGTCTCAAGAAGGCTCAGCTAGCAGCACCAAAAGCACAGAATTGTTACTAGGTGTTAAAACAATTCCAGATGATACACCAATGTGCCGTATACTCCTTCGCAAAGAAGTTCTAAGATTAGTCATTAATTTGAGTAGTTCAGTTTCAACTAAATGTCATGAGACTGGGCTTTTAACAATTAAGGAGAAGTATCCTCAAACATTTGATGACATATGCCTTTACTCTGAGGTTTCCCATTTGCTGTCACACTGCACATTCAGACTTCCGTGTCGGAGGTTCATACAAGAATTATTTCAAGATGTACAGTTTCTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAACACCACCAAAGCGACCTATAGTTGATACATCTGCTGAATCCTGACCGCGGCCGCGACTCTAGAGGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCC

Amino Acid Sequence

MYPYDVPDYAMAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLGWNWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGLSYLNERGYVAKQLEKWHREYNFKYVDLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSRERLVVESSTSSHMKIRSQGFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPSGTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKRPIVDTSAES

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU6616
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: There is no NCBI Acc. that is identical with our sequence , however all of the splicing variations exist either in some other accession number or in some EST; none of them are PCR generated mutations.; Changes compared to NM_152756; E545 IS G; F815 IS L; S837 IS F; S1219 IS G; Q1699 IS R ; ; AMPLIFIED IN 2 BANDS:; N terminal region from AA using EST RZPDCR749280; CterM: EST BQ049342 (IMAGE: 5787163)