cDNA Clone NKCC1 (shark)

• Plasmid: pGEX-NKCC (shark) 1-260-T175+179/+T184/AAA
• Parent Plasmid: pEBG6P
• Expressed: GST-NKCC (shark) 1-260-T175+179/+T184/AAA
• Genbank: A53491
• Tag: GST

Sequence of Insert

GAATTCGCCCTTGGATCCATGGAGCCCGCCTTTCCCGCTTCCTCGGCCGGCGTGCAGTCCCAGTCCGGCCCGGAGCCTGGTGCCGGCCAACAGGAGCCGCCGCCGCCGGCCACGCCGCTCCGTCCCGTAGCGAGTCAGAGCCGCTTTCAGGTCGACCTGGTGACCGAGGGTGGAGGTGGTGGAGACGGGCAGAAGGGACAGACCGCAGCCCAGCCTGCCGCCGCCGCCAAGGACAAGGACAGGGGGGACGGCGGTGCAGCGGCTCCGTCTCCAGCCTCACCTGCCGCCGCCGCCGAGCCGCCCGCCGCAGCAGCAGAAGAGGCGAAAGGCAGATTCCGGGTGAATTTCGTCGACCCGGCTTCGGATGAGCCGCCGCTGTCCAGCCAGCAGCAGCCGCCGCCGCCCAGCTCGGCATCGTCGGCGCACGGCGGCCACCAGCCGCCCAGCGAGAGCATGAACGGCTACCCGCAGAACGGCGATACCATGATGAGCGAGGGCAGCCTGCACTCGTCCGGCACCGGGGCTCACCATTACTACGATACGCACACCAACACTTACTATCTCAGGACTTTCGGCCACAACACCATCGACGCGGTGCCCAGGATCGATCATTACAGGCACACGGTGGCCCAACTGGGCGAGAAGCTCATCAGACCCAGCCTGGCTGAGCTACACGATGAGCTCGACAAGGAGCCTTTTGAGGATGGCTATGTAAATGGCGAAGAAAGCAGTCCAGCTGAAGAGGCTGTGTCGAAGCATGTTGCTGACAATAAAGGTGTTGTAAAGTTTGGCTGGATTTAAGCGGCCGCAAGGGCGAATTC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEPAFPASSAGVQSQSGPEPGAGQQEPPPPATPLRPVASQSRFQVDLVTEGGGGGDGQKGQTAAQPAAAAKDKDRGDGGAAAPSPASPAAAAEPPAAAAEEAKGRFRVNFVDPASDEPPLSSQQQPPPPSSASSAHGGHQPPSESMNGYPQNGDTMMSEGSLHSSGTGAHHYYDAHTNAYYLRAFGHNTIDAVPRIDHYRHTVAQLGEKLIRPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKGVVKFGWI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU6437
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee