cDNA Clone OSR1

• Plasmid: pCMV5-HA OSR
• Parent Plasmid: pCMV5
• Expressed: HA-OSR
• Genbank: NP_005100
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCCTTGGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGTCCGAAGACTCGAGCGCCCTGCCCTGGTCCATCAACAGGGACGATTACGAGCTGCAGGAGGTGATCGGGAGTGGAGCAACTGCTGTAGTCCAAGCAGCTTATTGTGCCCCTAAAAAGGAGAAAGTGGCAATCAAACGGATAAACCTTGAGAAATGTCAAACTAGCATGGATGAACTCCTGAAAGAAATTCAAGCCATGAGTCAATGCCATCATCCTAATATTGTATCTTACTACACATCTTTTGTGGTAAAAGATGAGCTGTGGCTTGTCATGAAGCTGCTAAGTGGAGGTTCTGTTCTGGATATTATTAAGCACATTGTGGCAAAAGGGGAACACAAAAGTGGAGTCCTAGATGAATCTACCATTGCTACGATACTCCGAGAAGTACTGGAAGGGCTGGAATATCTGCATAAAAATGGACAGATCCACAGAGATGTGAAAGCTGGAAACATTCTTCTTGGAGAAGATGGCTCAGTACAGATTGCAGACTTTGGGGTTAGTGCTTTTTTAGCAACTGGTGGTGATATTACCCGAAATAAAGTGAGAAAGACCTTTGTTGGCACCCCTTGTTGGATGGCACCTGAAGTTATGGAACAGGTCCGTGGTTATGATTTCAAAGCTGATATTTGGAGTTTTGGAATTACAGCAATTGAATTGGCTACAGGGGCGGCTCCTTATCATAAATATCCACCAATGAAGGTTTTAATGCTGACACTGCAGAACGATCCTCCTTCTTTGGAAACTGGTGTTCAAGATAAAGAAATGCTGAAAAAATATGGAAAATCATTTAGAAAAATGATTTCATTGTGCCTTCAAAAAGATCCAGAAAAAAGACCAACAGCAGCAGAACTATTAAGGCACAAATTTTTCCAGAAAGCAAAGAATAAAGAATTTCTTCAAGAAAAAACATTGCAGAGAGCACCAACCATTTCTGAAAGAGCAAAAAAGGTTCGGAGGGTACCAGGTTCCAGTGGGCGTCTTCATAAGACAGAGGATGGAGGCTGGGAGTGGAGTGATGATGAATTTGATGAAGAAAGTGAGGAAGGGAAAGCAGCAATTTCACAACTCAGGTCTCCCCGAGTGAAAGAATCAATATCAAATTCTGAGCTCTTTCCAACAACTGATCCTGTGGGTACTTTGCTCCAAGTTCCAGAACAGATCTCTGCTCATCTACCTCAGCCAGCTGGGCAGATTGCTACACAGCCAACTCAAGTCTCTCTCCCACCCACCGCAGAGCCAGCAAAAACAGCTCAGGCTTTGTCTTCAGGATCAGGTTCACAAGAAACCAAGATCCCAATCAGTCTAGTACTAAGATTAAGGAATTCCAAAAAAGAACTAAATGATATTCGATTTGAATTTACTCCTGGGAGAGATACAGCAGAGGGTGTCTCTCAGGAACTCATTTCTGCTGGCCTGGTCGACGGAAGGGATTTAGTAATAGTGGCAGCTAATTTGCAGAAAATTGTGGAAGAACCTCAGTCAAATCGATCTGTCACTTTCAAACTGGCGTCTGGTGTCGAAGGCTCAGATATTCCTGATGATGGTAAACTGATAGGATTTGCCCAGCTCAGCATCAGCTAA

Amino Acid Sequence

MYPYDVPDYAMSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU2886
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Tha cDNA was amplified from EST NCBI Acc. BI464286 IMAGE5271312 Using the following primers:; ; 1.GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGTCCGAAGACTCGAGCGCCCTGCCCT; ; 2.GCGGCCGCTTAGCTGATGCTGAGCTGGGCAAATCC; PCMV-Bam-Not modified DU2865; This EST is a wrongly spliced cDNA containing some intron bits. The 100 nt intron peace and an amino acid mutation in the coding region was corrected by PCR methods to create a sequence that is 100% identical with NCBI NP_005100