cDNA Clone GSK3 alpha (mouse)

• Synonyms: GSK3A
• Plasmid: pEBG-2T-GSKa (mouse) wt
• Parent Plasmid: pEBG2T
• Expressed: GST-GSK3 alpha (mouse)
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGAGCGGCGGCGGGCCTTCGGGAGGCGGCCCTGGGGGCTCGGGCCGGGCGCGGACCAGCTCGTTCGCGGAGCCAGGAGGCGGAGGCGGAGGTGGTGGCGGCGGCCCCGGGGGCTCGGCCTCTGGCCCAGGAGGCACTGGCGGCGGGAAAGCGTCAGTCGGGGCTATGGGTGGGGGCGTGGGAGCCTCGAGCTCCGGGGGTGGCCCCAGCGGCAGCGGCGGAGGAGGCAGCGGTGGCCCCGGCGCGGGCACTAGCTTCCCGCCGCCGGGAGTGAAGCTGGGCCGTGACAGCGGGAAGGTGACCACAGTGGTAGCCACTGTAGGCCAAGGCCCAGAGCGTTCCCAAGAAGTGGCTTACACTGACATCAAAGTGATTGGTAATGGCTCATTCGGAGTAGTATACCAGGCACGGCTGGCAGAGACGAGGGAACTGGTGGCCATCAAGAAGGTTCTTCAGGACAAAAGGTTCAAGAACCGAGAACTGCAGATTATGCGTAAGCTGGACCACTGCAATATTGTGAGGCTGCGGTACTTTTTCTACTCCAGTGGGGAGAAGAAGGACGAGCTGTATTTGAATCTGGTGCTGGAGTATGTGCCCGAGACGGTGTACCGAGTGGCCCGCCACTTCACCAAGGCCAAGCTGATCACCCCTATCATCTACATCAAGGTGTACATGTACCAGCTCTTCCGGAGCTTGGCCTACATCCACTCCCAAGGTGTGTGTCACCGTGACATCAAGCCCCAGAATTTGCTTGTGGACCCTGACACTGCTGTCCTCAAGCTCTGCGATTTTGGCAGTGCAAAGCAGCTGGTTCGGGGGGAGCCCAATGTGTCCTACATCTGCTCTCGGTACTACCGTGCTCCAGAACTCATCTTTGGAGCCACAGATTACACCTCGTCCATCGATGTGTGGTCGGCTGGCTGTGTACTTGCTGAGCTACTTCTCGGCCAGCCCATCTTCCCTGGGGACAGTGGGGTGGACCAGCTTGTGGAGATCATCAAGGTACTAGGAACGCCAACCAGGGAACAAATCCGAGAGATGAACCCTAACTATACGGAGTTCAAGTTCCCCCAGATCAAAGCTCACCCTTGGACAAAGGTGTTCAAATCTTCAAAGACACCACCTGAGGCCATTGCACTCTGCTCTAGCCTACTGGAGTACACGCCATCCTCAAGGCTCTCCCCACTCGAGGCTTGTGCCCACAGCTTCTTCGATGAACTGCGGAGACTCGGAGCCCAGCTCCCCAACGACCGCCCGCTTCCCCCCCTGTTCAACTTCAGTCCTGGTGAACTGTCCATCCAACCATCTCTCAATGCCATTCTCATCCCTCCTCACTTGAGGTCCCCAGCAGGCCCTGCTTCTCCCCTCACCACTTCCTACAACCCATCCTCACAAGCTTTAACTGAAGCTCAGACTGGCCAAGATTGGCAGCCATCTGATGCCACAACTGCTACCCTCGCTAGCTCTTCCTGAGGGCCCCACCGACTACCCCTCCACGTCCATCTGGGAAGGCCCAAGTGCGTGTGGAGAGAGGGGGCCACAGCCCATCAAGCTCCTGCCCTGGGCTGGGCCCCTAGACTAGAGGGCAGAGGTAAATGAACTACCCCCAGCATCTAGGCCTCCCTCAGCAGCCTACCTTCTGTGGTGGGCTTTTTAAAGAGGATTTACTGTTGGTAGGGGGAGGGGAGGAAAATGAGGGACGGGGTTTTGGGGGGTGTGAGGACCCTCTTACCCCATTGGATCCCTCCCCTCCCCCCATGTCTACACATCATACCCTCCCCCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSMSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPSGSGGGGSGGPGAGTSFPPPGVKLGRDSGKVTTVVATVGQGPERSQEVAYTDIKVIGNGSFGVVYQARLAETRELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLITPIIYIKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKSSKTPPEAIALCSSLLEYTPSSRLSPLEACAHSFFDELRRLGAQLPNDRPLPPLFNFSPGELSIQPSLNAILIPPHLRSPAGPASPLTTSYNPSSQALTEAQTGQDWQPSDATTATLASSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU2687
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 0.5 Kb of n-domain amplified from EST NCBI Acc. BQ 964176 IMAGE 6513981, then ligated to the vector as Bamh1-Pst (from the 0.5 Kb Topo clone) + 1.6 Kb PSt-Not fragment from the same EST; NNN-S have no good quality sequence from the non-translated 3’ end.