cDNA Clone NUAK2

• Synonyms: SNARK
• Plasmid: pGFP TAP HA NUAK2
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP TAP 3C HA NUAK2
• Genbank: NM_030952.3
• Tag: HA
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCACTAGTGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGAAGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGTGAAGAAGGCGCGGGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAGATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGAAGTGTTTGAGAACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTACATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTCTCTGCCGTGCACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGGATGCCAATGGGAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTACCATCAAGGCAAGTTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTCGCCAGAGATTGTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCTGGTCCCTGGGTGTTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGATCCTAGTGAAACAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATCCGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCACTGGTGGGTCAACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGTGGGCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTGTGCAGCTTCTTCAAGCAGCATGCACCTGGTGGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGCTCAAGAAGTCCCGCAAGGAGAATGACATGGCCCAGTCCCTCCACAGTGACACGGCTGATGACACTGCCCATCGCCCTGGCAAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAAGGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCCCCCCTGCTCCCCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTACTCCTCTCCCGAGCCCAGTGAATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGTGGGGATCCCAAGGAGCAGAAGCCTCCGCAAGCTTCAGGGCTGCTCCTCCATCGCAAAGGCATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGGAGCTCGCGGCCCCCACCACCTTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAGCCGACCCTCAGGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCTGAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTTGAGGAGCCCCCCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTGGGGGACAGCTGCTTTTCCCTGACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCACTGAGGGTCTGCTCAAAGCTCACCTGAAAGGGCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGTACCATCGATGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSFDIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSTSATMYPYDVPDYAESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAV
KRHHHKHNLRHRYEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDY
ISERQQLSEREARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLL
YILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSS
RPLLENGAKVCSFFKQHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAEGVQEDPPELSPIPASPGQAA
PLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPS
GAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT*

• Vector Type: Mammalian
• Antibiotic: Kan
• Full Snapgene Map: pGFP TAP HA NUAK2 63.64 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU2172
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference sequence there are two silent changes at G195 and S386.
• Low Temp: No