cDNA Clone MARK2

• Plasmid: pCMV5-HA MARK2
• Parent Plasmid: pCMV5
• Expressed: HA-MARK2
• Genbank: NM_004954
• Tag: HA
• Species: Human

Sequence of Insert

ggtaccgccaccatgtacccatacgatgtgccagattacgccattcgggg
ccgcaactcagccacctctgctgatgagcagccccacattggaaactacc
ggctcctcaagaccattggcaagggtaattttgccaaggtgaagttggcc
cgacacatcctgactgggaaagaggtagctgtgaagatcattgacaagac
tcaactgaactcctccagcctccagaaactattccgcgaagtaagaataa
tgaaggttttgaatcatcccaacatagttaaattatttgaagtgattgag
actgagaaaacgctctaccttgtcatggagtacgctagtggcggagaggt
atttgattacctagtggctcatggcaggatgaaagaaaaagaggctcgag
ccaaattccgccagatagtgtctgctgtgcagtactgtcaccagaagttt
attgtccatagagacttaaaggcagaaaacctgctcttggatgctgatat
gaacatcaagattgcagactttggcttcagcaatgaattcacctttggga
acaagctggacaccttctgtggcagtcccccttatgctgccccagaactc
ttccagggcaaaaaatatgatggacccgaggtggatgtgtggagcctagg
agttatcctctatacactggtcagcggatccctgccttttgatggacaga
acctcaaggagctgcgggaacgggtactgaggggaaaataccgtattcca
ttctacatgtccacggactgtgaaaacctgcttaagaaatttctcattct
taatcccagcaagagaggcactttagagcaaatcatgaaagatcgatgga
tgaatgtgggtcacgaagatgatgaactaaagccttacgtggagccactc
cctgactacaaggacccccggcggacagagctgatggtgtccatgggtta
tacacgggaagagatccaggactcgctggtgggccagagatacaacgagg
tgatggccacctatctgctcctgggctacaagagctccgagctggaaggc
gacaccatcaccctgaaaccccggccttcagctgatctgaccaatagcag
cgccccatccccatcccacaaggtacagcgcagcgtgtcggccaatccca
agcagcggcgcttcagcgaccaggctggtcctgccattcccacctctaat
tcttactctaagaagactcagagtaacaacgcagaaaataagcggcctga
ggaggaccgggagtcagggcggaaagccagcagcacagccaaggtgcctg
ccagccccctgcccggtctggagaggaagaagaccaccccaaccccctcc
acgaacagcgtcctctccaccagcacaaatcgaagcaggaattccccact
tttggagcgggccagcctcggccaggcctccatccagaatggcaaagaca
gcacagccccccagcgtgtccctgttgcctccccatccgcccacaacatc
agcagcagtggtggagccccagaccgaactaacttcccccggggtgtgtc
cagccgaagcaccttccatgctgggcagctccgacaggtgcgggaccagc
agaatttgccctacggtgtgaccccagcctctccctctggccacagccag
ggccggcggggggcctctgggagcatcttcagcaagttcacctccaagtt
tgtacgcaggaacctgaatgaacctgaaagcaaagaccgagtggagacgc
tcagacctcacgtggtgggcagtggcggcaacgacaaagaaaaggaagaa
tttcgggaggccaagccccgctccctccgcttcacgtggagtatgaagac
cacgagctccatggagcccaacgagatgatgcgggagatccgcaaggtgc
tggacgcgaacagctgccagagcgagctgcatgagaagtacatgctgctg
tgcatgcacggcacgccgggccacgaggacttcgtgcagtgggagatgga
ggtgtgcaaactgccgcggctctctctcaacggggttcgatttaagcgga
tatcgggcacctccatggccttcaaaaacattgcctccaaaatagccaac
gagctgaagctttaatctaga

Amino Acid Sequence

MYPYDVPDYAIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHIL
TGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKT
LYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHR
DLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGK
KYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMS
TDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYK
DPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTIT
LKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRFSDQAGPAIPTSNSYSK
KTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSV
LSTSTNRSRNSPLLERASLGQASIQNGKDSTAPQRVPVASPSAHNISSSG
GAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRG
ASGSIFSKFTSKFVRRNLNEPESKDRVETLRPHVVGSGGNDKEKEEFREA
KPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHG
TPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1253
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Changes to NM_004954 ORF sequence: ; g696a (silent in G232); c759t (silent in I253); a1050g (silent in L350)*; a1067c (Q356P)*; t1070c (F357S)*; a1092c (silent in R364)*; * natural variants