cDNA Clone BRSK1

• Plasmid: pCMV5-HA BRSK1 T189A
• Parent Plasmid: pCMV5
• Expressed: HA-BRSK1 T189A
• Genbank: NM_032430
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCTCGTCCGGGGCCAAGGAGGGAGGTGGGGGCTCTCCCGCCTACCACCTCCCCCACCCCCACCCCCACCCACCCCAGCACGCCCAATATGTGGGCCCCTATCGGCTGGAGAAGACGCTGGGCAAAGGACAGACAGGGCTGGTTAAACTCGGGGTCCACTGCATCACGGGTCAGAAGGTCGCCATCAAGATCGTGAACCGGGAGAAGCTGTCGGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCCATCCTGAAGCTCATCGAACACCCACATGTCCTCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTGTACCTGGTTCTGGAGCACGTCTCGGGGGGTGAGCTATTCGACTACCTGGTAAAGAAGGGGAGACTGACGCCCAAGGAGGCCCGAAAGTTCTTCCGCCAGATTGTGTCTGCGCTGGACTTCTGCCACAGCTACTCCATCTGCCACAGAGACCTAAAGCCCGAGAACCTGCTTTTGGATGAGAAAAACAACATCCGCATTGCAGACTTCGGCATGGCGTCCCTGCAGGTGGGGGACAGCCTCCTGGAGgCCAGCTGCGGGTCCCCCCATTATGCGTGTCCAGAGGTGATTAAGGGGGAAAAATATGATGGCCGCCGGGCAGACATGTGGAGCTGTGGAGTCATCCTCTTCGCCCTGCTCGTGGGGGCTCTGCCCTTTGATGACGACAACCTCCGCCAGCTGCTGGAGAAGGTGAAACGGGGCGTCTTCCACATGCCCCACTTCATTCCTCCAGATTGCCAGAGCCTCCTGAGGGGAATGATCGAAGTGGAGCCCGAAAAAAGGCTCAGTCTGGAGCAAATTCAGAAACATCCTTGGTACCTAGGCGGGAAACACGAGCCAGACCCGTGCCTGGAGCCAGCCCCTGGCCGCCGGGTAGCCATGCGGAGCCTGCCATCCAACGGAGAGCTGGACCCCGACGTCCTAGAGAGCATGGCATCACTGGGCTGCTTCAGGGACCGCGAGAGGCTGCATCGCGAGCTGCGCAGTGAGGAGGAGAACCAAGAAAAGATGATATATTATCTGCTTTTGGATCGGAAGGAGCGGTATCCCAGCTGTGAGGACCAGGACCTGCCTCCCCGGAATGATGTTGACCCCCCCCGGAAGCGTGTGGATTCTCCCATGCTGAGCCGTCACGGGAAGCGGCGACCAGAGCGGAAGTCCATGGAAGTCCTGAGCATCACCGATGCCGGGGGTGGTGGCTCCCCTGTACCCACCCGACGGGCCTTGGAGATGGCCCAGCACAGCCAGAGATCCCGTAGCGTCAGTGGAGCCTCCACGGGTCTGTCCTCCAGCCCTCTAAGCAGCCCAAGGAGTCCGGTCTTTTCCTTTTCACCGGAGCCGGGGGCTGGAGATGAGGCTCGAGGCGGGGGCTCCCCGACTTCCAAAACGCAGACGCTGCCTTCTCGGGGCCCCAGGGGTGGGGGCGCCGGGGAGCAGCCCCCGCCCCCCAGTGCCCGCTCCACACCCCTGCCCGGCCCCCCAGGCTCCCCGCGCTCCTCTGGCGGGACCCCCTTGCACTCGCCTCTGCACACGCCCCGGGCCAGTCCCACCGGGACCCCGGGGACAACACCACCCCCCAGCCCCGGCGGTGGCGTCGGGGGAGCCGCCTGGAGGAGTCGTCTCAACTCCATCCGCAACAGCTTCCTGGGCTCCCCTCGCTTTCACCGGCGCAAGATGCAGGTCCCTACCGCTGAGGAGATGTCCAGCTTGACGCCAGAGTCCTCCCCGGAGCTGGCAAAACGCTCCTGGTTCGGGAACTTCATCTCCTTGGACAAAGAAGAACAAATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCAGACATCGTCCATGCCTTTCTGTCGATCCCCAGCCTGAGTCACAGTGTGCTGTCACAGACCAGCTTCAGGGCCGAGTACAAGGCCAGTGGCGGCCCCTCCGTCTTCCAAAAGCCCGTCCGCTTCCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAGCCCTCCCCGCGACGGGACGGCAGCGGAGGTGGTGGCATCTACTCCGTCACCTTCACTCTCATCTCGGGTCCCAGCCGTCGGTTCAAGCGAGTGGTGGAGACCATCCAGGCACAGCTCCTGAGCACTCATGACCAGCCCTCCGTGCAGGCCCTGGCAGACGAGAAGAACGGGGCCCAGACCCGGCCTGCTGGTGCCCCACCCCGAAGCCTGCAGCCCCCACCCGGCCGCCCAGACCCAGAGCTGAGCAGCTCTCCCCGCCGAGGCCCCCCCAAGGACAAGAAGCTCCTGGCCACCAACGGGACCCCTCTGCCCTGAggatcc

Amino Acid Sequence

MYPYDVPDYASSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLG
KGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHP
HVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIV
SALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLEASC
GSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLE
KVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHE
PDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRS
EEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHG
KRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLS
SSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQ
PPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPG
GGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELA
KRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQ
TSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVT
FTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPP
RSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1242
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee