cDNA Clone TBC1D1

• Plasmid: pGEX6P-3-TBC1D1
• Parent Plasmid: pGEX6P3
• Expressed: GST-TBC1D1
• Genbank: NM_015173
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAACGAGGTCTCGGTGGATTTTGGCCTGCAGCTGGTGGGCTCCCTGCCTGTGCATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGTGCGAAGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCGGCTTTGCGTTTCACCCTCTGGACTGAGATGTGAACCTGAGCCAGGGAGAAGTCAACAGTGGGATCCCCTGATCTATTCCAGCATCTTTGAGTGCAAGCCTCAGCGT
GTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGCTTGTCT
GATTAAGGAAGACGCTGTCCACCGGCAGAGTATCTGCTATGTGTTCAAAG
CCGATGATCAAACAAAAGTGCCTGAGATCATCAGCTCCATCCGTCAGGCG
GGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCGAGTTCGACGA
CACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGACGGTGG
CGCACAAGAAGGCTCCGCCGGCCCTGATCGACGAGTGCATCGAGAAGTTC
AATCACGTCAGCGGCAGCCGGGGGTCCGAGAGCCCCCGCCCCAACCCGCC
CCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCATGCGCA
AGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAGGAGCTG
CAGGATGGGGGCCTCCGAAGCAGCGGCTTCTTCAGCTCCTTCGAGGAGAG
CGACATTGAGAACCACCTCATTAGCGGACACAATATTGTGCAGCCCACAG
ATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCTGAAGTT
TACCTCATCAGTCCTGACACCAAAAAAATAGCATTGGAGAAAAATTTTAA
GGAGATATCCTTTTGCTCTCAGGGCATCAGACACGTGGACCACTTTGGGT
TTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTCTGTTAC
GTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGATGACCCT
GAAACAGGCCTTCACGGTGGCCGCAGTGCAGCAGACAGCTAAGGCGCCAG
CCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAAGCTCTGTGAG
AGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCAAAAGCA
CCTGACGACATTAACAAATCAGGAGCAGGCGACTATTTTTGAAGAGGTTC
AGAAATTGAGACCGAGAAATGAGCAGCGAGAGAATGAATTGATTATTTCT
TTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCATATTGG
GGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAAGTGAAT
TACCACCCAGTGCCACTCGATTTAGGCTAGATATGCTGAAAAACAAAGCA
AAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCCGGGGTAATAA
AGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATAGCTCCC
TGTCTAGTACATTAAGTAACACCAGCAAAGAGCCATCTGTGTGTGAAAAG
GAGGCCTTGCCCATCTCTGAGAGCTCCTTTAAGCTCCTCGGCTCCTCGGA
GGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAGCTCCGC
TGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGTCACTTC
CCCATCGAATGCCAGGAACCTCCACAACCTGCCCGGGGGTCCCCGGGGGT
TTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACAGAGACGCCTC
ATGAACGAAAGGACTTTGAATCCAAAGCAAACCATCTTGGTGATTCTGGT
GGGACTCCTGTGAAGACCCGGAGGCATTCCTGGAGGCAGCAGATATTCCT
CCGAGTAGCCACCCCGCAGAAGGCGTGCGATTCTTCCAGCAGATATGAAG
ATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAACCAGTT
TGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAGGACATC
TCGTGAGCTCCGAGAGCTGTGGCAAAAGGCTATTCTTCAACAGATACTGC
TGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCCTCTGAAAATGAT
TTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCCCTGTCT
TAAAGAAGTAACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGAT
CAAAAATTAAGTTTGACATGGAAAAAATGCACTCGGCTGTTGGGCAAGGT
GTGCCACGTCATCACCGAGGTGAAATCTGGAAATTTCTAGCTGAGCAATT
CCACCTTAAACACCAGTTTCCCAGCAAACAGCAGCCAAAGGATGTGCCAT
ACAAAGAACTCTTAAAGCAGCTGACTTCCCAGCAGCATGCGATTCTTATT
GACCTTGGGCGAACCTTTCCTACACACCCATACTTCTCTGCCCAGCTTGG
AGCAGGACAGCTATCGCTTTACAACATTTTGAAGGCCTACTCACTTCTAG
ACCAGGAAGTGGGATATTGCCAAGGTCTCAGCTTTGTAGCAGGCATTTTG
CTTCTTCATATGAGTGAGGAAGAGGCGTTTAAAATGCTCAAGTTTCTGAT
GTTTGACATGGGGCTGCGGAAACAGTATCGGCCAGACATGATTATTTTAC
AGATCCAGATGTACCAGCTCTCGAGGTTGCTTCATGATTACCACAGAGAC
CTCTACAATCACCTGGAGGAGCACGAGATCGGCCCCAGCCTCTACGCTGC
CCCCTGGTTCCTCACCATGTTTGCCTCACAGTTCCCGCTGGGATTCGTAG
CCAGAGTCTTTGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAA
GTGGCTTTAAGTCTGTTGGGAAGCCATAAGCCCTTGATTCTGCAGCATGA
AAACCTAGAAACCATAGTTGACTTTATAAAAAGCACGCTACCCAACCTTG
GCTTGGTACAGATGGAAAAGACCATCAATCAGGTATTTGAAATGGACATC
GCTAAACAGTTACAAGCTTATGAAGTTGAGTACCACGTCCTTCAAGAAGA
ACTTATCGATTCCTCTCCTCTCAGTGACAACCAAAGAATGGATAAATTAG
AGAAAACCAACAGCAGCTTACGCAAACAGAACCTTGACCTCCTTGAACAG
TTGCAGGTGGCAAATGGTAGGATCCAAAGCCTTGAGGCCACCATTGAGAA
GCTCCTGAGCAGTGAGAGCAAGCTGAAGCAGGCCATGCTTACCTTAGAAC
TGGAGCGGTCGGCCCTGCTGCAGACGGTGGAGGAGCTGCGGCGGCGGAGC
GCAGAGCCCAGCGACCGGGAGCCTGAGTGCACGCAGCCCGAGCCCACGGG
CGACTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMEPITFTARKHLL
SNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQ
VRLCVSPSGLRCEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSY
FACLIKEDAVHRQSICYVFKADDQTKVPEIISSIRQAGKIARQEELHCPS
EFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSESPR
PNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSS
FEESDIENHLISGHNIVQPTDIEENRTMLFTIGQSEVYLISPDTKKIALE
KNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCYVFQCTNEALVDEI
MMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLE
LQKHLTTLTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEH
IHIGEMKQTSQMAAENIGSELPPSATRFRLDMLKNKAKRSLTESLESILS
RGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSFKLL
GSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARG
SPGVSQRKLMRYHSVSTETPHERKDFESKANHLGDSGGTPVKTRRHSWRQ
QIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPVCEDGPFGPPPEEK
KRTSRELRELWQKAILQQILLLRMEKENQKLQASENDLLNKRLKLDYEEI
TPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFL
AEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFS
AQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKML
KFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPS
LYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLI
LQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHV
LQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEA
TIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQP
EPTGD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11184
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422); ; ; Estimated MW of expressed protein : 160573 Daltons