cDNA Clone slit-robo-GAP 3

• Plasmid: pCMV5-HA slit-robo-GAP 3a residues 1-803 (nt1-2409)
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-slit-robo-GAP 3a residues 1-803 (nt1-2409)
• Genbank: NM_014850
• Tag: HA
• Species: Human

Sequence of Insert

gaattcatgtcatctcaaactaagttcaagaaagacaaagagatcattgctgaatatgaagcccaaataaaagagatccgcacgcagctggtggagcagttcaaatgtctggagcagcaatcagagtcgcgactgcagctgcttcaagacctccaggagtttttccgccggaaagctgagattgagctcgagtactcccgcagcctggagaagctggctgagcgcttctcctccaaaatccgcagctcccgggagcaccagttcaagaaggaccagtacctcctctcgcctgtgaactgttggtat
ctggttctgcatcagacccggcgggagagccgagaccatgccaccctcaa
tgacatcttcatgaacaatgtcatcgtccgcctctcccagatcagtgagg
atgtcatcagactcttcaaaaagagcaaggagattggcctgcagatgcac
gaggagctcctgaaggtgaccaatgagctctacacagtcatgaaaaccta
ccacatgtaccatgcagagagcatcagtgcggaaagcaagctgaaggagg
ctgagaagcaggaggagaagcagttcaataagtcaggagacctcagcatg
aacctgctccggcacgaggaccggccccagcgccgcagctctgtgaagaa
gattgagaagatgaaggagaagaggcaggccaagtactctgagaacaagc
tgaaatgcacaaaggcccggaatgactacttgctcaatctggcagccacc
aacgcagctataagcaaatactacatccatgatgtctctgatctgatcga
ttgctgtgatttgggcttccatgccagcctggcccgcaccttccggacct
atctctcagctgaatacaacctggagacctctcgccacgaagggctggat
gtcattgagaatgcagtggacaacctggattcccgaagtgacaagcacac
agtcatggacatgtgcaatcaagtcttctgccctccactcaagttcgagt
tccagccccacatgggggatgaggtctgccaggtcagcgctcagcagccc
gtccagacagaactgctcatgcgttatcaccagctgcagtccagactggc
caccctcaagatagagaatgaggaggttaggaaaaccctggatgccacca
tgcagacattacaggacatgctgactgtggaggactttgatgtctccgat
gccttccaacacagtcgatcgacagagtccgtcaagtcggctgcctctga
gacctacatgagcaagatcaacattgccaagaggagagccaaccagcagg
aaacagaaatgttttattttacaaaatttaaagagtatgtgaatggcagt
aacctcatcaccaagctgcaggccaagcacgatttactcaagcagaccct
gggcgaaggggaaagagcagaatgcggcaccaccaggcccccctgtcttc
cccctaaaccacagaaaatgaggagacctaggcctctctcagtgtatagc
cataaactctttaacggcagtatggaagcattcattaaggattcaggaca
agctataccgcttgtagtcgagagctgcatccgttacatcaatttatatg
gactccagcagcaggggatcttcagagtgccaggatctcaggtggaagtc
aatgacatcaaaaattcctttgagagaggtgaagacccccttgtggacga
tcaaaatgaacgagatatcaattcagtcgctggtgttttaaaactgtatt
tccgaggactggaaaacccactctttcctaaggaaaggtttcaagatttg
atatctactattaaactggagaacccagccgagagggtgcaccagatcca
acaaatcctcgtcacccttccccgcgtggtcattgtggtcatgagatacc
tcttcgctttcctcaaccacctctcccagtatagcgacgagaacatgatg
gatccctacaacctggccatctgcttcgggcctaccctcatgcacatccc
tgatgggcaggaccctgtgtcctgccaggcacacatcaatgaagtcatca
aaaccatcatcatccatcatgaagccatcttccccagcccccgggagcta
gagggacctgtgtatgaaaaatgcatggctggaggggaagaatattgtga
cagcccacacagcgagccaggggccatcgatgaagttgaccatgacaatg
gcactgagcctcataccagcgatgaagaagtggagcagatcgaggctatt
gccaagtttgactacatggggcggtccccgcgtgagctatccttcaagaa
gggggcctcgctgctcctgtaccaccgcgcctcggaggactggtgggagg
gccggcacaacggcgtggatggactcatcccccatcagtacatagttgta
caggacatgtga…..

Amino Acid Sequence

MYPYDVPDYAGSPEFMSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCL
EQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKLAERFSSKIRSSREHQ
FKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDV
IRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAE
KQEEKQFNKSGDLSMNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLK
CTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYL
SAEYNLETSRHEGLDVIENAVDNLDSRSDKHTVMDMCNQVFCPPLKFEFQ
PHMGDEVCQVSAQQPVQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQ
TLQDMLTVEDFDVSDAFQHSRSTESVKSAASETYMSKINIAKRRANQQET
EMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPP
KPQKMRRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRYINLYGL
QQQGIFRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFR
GLENPLFPKERFQDLISTIKLENPAERVHQIQQILVTLPRVVIVVMRYLF
AFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHINEVIKT
IIIHHEAIFPSPRELEGPVYEKCMAGGEEYCDSPHSEPGAIDEVDHDNGT
EPHTSDEEVEQIEAIAKFDYMGRSPRELSFKKGASLLLYHRASEDWWEGR
HNGVDGLIPHQYIVVQDM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11183
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Unidentified 780 bp dna sequence fused to 3’ end of GAP3 insert, not translated as STOP codon at end of GAP3 sequence. ; ; Estimated MW of expressed protein : 94828 Daltons