cDNA Clone M-RIP

• Plasmid: pCMV5-HA M-RIP
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-M-RIP
• Genbank: NM_015134
• Tag: HA
• Species: Human

Sequence of Insert

gaattcatgtcggcagccaaggagaacccgtgcaggaaattccaggccaacatcttcaacaagagcaagtgtcagaactgcttcaagccccgcgagtcgcatctgctcaacgacgaggacctgacgcaggcaaaacccatttatggcggttggctgctcctggctccagatgggaccgactttgacaacccagtgcaccggtctcggaaatggcagcgacggttcttcatcctttacgagcacggcctcttgcgctacgccctggatgagatgcccacgacccttcctcagggcaccatcaacatg
aaccagtgcacagatgtggtggatggggagggccgcacgggccagaagtt
ctccctgtgtattctgacgcctgagaaggagcatttcatccgggcggaga
ccaaggagatcgtcagtgggtggctggagatgctcatggtctatccccgg
accaacaagcagaatcagaagaagaaacggaaagtggagccccccacacc
acaggagcctgggcctgccaaggtggctgttaccagcagcagcagcagca
gcagcagcagcagcatccccagtgctgagaaagtccccaccaccaagtcc
acactctggcaggaagaaatgaggaccaaggaccagccagatggcagcag
cctgagtccagctcagagtcccagccagagccagcctcctgctgccagct
ccctgcgggaacctgggctagagagcaaagaagaggagagcgccatgagt
agcgaccgcatggactgtggccgcaaagtccgggtggagagcggctactt
ctctctggagaagaccaaacaggacttgaaggctgaagaacagcagctgc
ccccgccgctctcccctcccagccccagcacccccaaccacaggaggtcc
caggtgattgaaaagtttgaggccttggacattgagaaggcagagcacat
ggagaccaatgcagtggggccctcacaatccagcgacacacgccagggcc
gcagcgagaagagggcgttccctaggaagcgggacttcaccaatgaagcc
cccccagctcctctcccagacgcctcggcttcccccctgtctccacaccg
aagagccaagtcactggacaggaggtccacggagccctccgtgacgcccg
acctgctgaatttcaagaaaggctggctgactaagcagtatgaggacggc
cagtggaagaaacactggtttgtcctcgccgatcaaagcctgagatacta
cagggattcagtggctgaggaggcagccgacttggatggagaaattgact
tgtccgcatgttacgatgtcacagagtatccagttcagagaaactatggc
ttccagatacatacaaaggagggcgagtttaccctgtcggccatgacatc
tgggattcggcggaactggatccagaccatcatgaagcacgtgcacccga
ccactgccccggatgtgaccagctcgttgccagaggaaaaaaacaagagc
agctgctcttttgagacctgcccgaggcctactgagaagcaagaggcaga
gctgggggagccggaccctgagcagaagaggagccgcgcacgggagcgga
ggcgagagggccgctccaagacctttgactgggctgagttccgtcccatc
cagcaggccctggctcaggagcgggtgggcggcgtggggcctgctgacac
ccacgagcccctgcgccctgaggcggagcctggggagctggagcgggagc
gtgcacggaggcgggaggagcgccgcaagcgcttcgggatgctcgacgcc
acagacgggccaggcactgaggatgcagccctgcgcatggaggtggaccg
gagcccagggctgcctatgagcgacctcaaaacgcataacgtccacgtgg
agattgagcagcggtggcatcaggtggagaccacacctctccgggaagag
aagcaggtgcccatcgcccccgtccacctgtcttctgaagatgggggtga
ccggctctccacacacgagctgacctctctgctcgagaaggagctggagc
agagccagaaggaggcctcagaccttctggagcagaaccggctcctgcag
gaccagctgagggtggccctgggccgggagcagagcgcccgtgagggcta
cgtgctgcaggccacgtgcgagcgagggtttgcagcaatggaagaaacgc
accagaagaagattgaagatctccagaggcagcaccagcgggagctagag
aaacttcgagaagagaaagaccgcctcctagccgaggagacagcggccac
catctcagccatcgaagccatgaagaacgcccaccgggaggaaatggagc
gggagctggagaagagccagcggtcccagatcagcagcgtcaactcggat
gttgaggccctgcggcgccagtacctggaggagctgcagtcggtgcagcg
ggaactggaggtcctctcggagcagtactcgcagaagtgcctggagaatg
cccatctggcccaggcgctggaggccgagcggcaggccctgcggcagtgc
cagcgtgagaaccaggagctcaatgcccacaaccaggagctgaacaaccg
cctggctgcagagatcacacggttgcggacgctgctgactggggacggcg
gtggggaggccactgggtcaccccttgcacagggcaaggatgcctatgaa
ctagaggtcttattgcgggtaaaggaatcggaaatacagtacctgaaaca
ggagattagctccctcaaggatgagctgcagacggcactgcgggacaaga
agtacgcaagtgacaagtacaaagacatctacacagagctcagcatcgcg
aaggctaaggctgactgtgacatcagcaggttgaaggagcagctcaaggc
tgcaacggaagcactgggggagaagtcccctgacagtgccacggtgtccg
gatatgatataatgaaatctaaaagcaaccctgacttcttgaagaaagac
agatcctgtgtcacccggcaactcagaaacatcaggtccaagagtctgaa
ggaaggcctgacggtgcaagaacggttgaagctctttgaatccagggact
tgaagaaagactaggcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPEFMSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLN
DEDLTQAKPIYGGWLLLAPDGTDFDNPVHRSRKWQRRFFILYEHGLLRYA
LDEMPTTLPQGTINMNQCTDVVDGEGRTGQKFSLCILTPEKEHFIRAETK
EIVSGWLEMLMVYPRTNKQNQKKKRKVEPPTPQEPGPAKVAVTSSSSSSS
SSSIPSAEKVPTTKSTLWQEEMRTKDQPDGSSLSPAQSPSQSQPPAASSL
REPGLESKEEESAMSSDRMDCGRKVRVESGYFSLEKTKQDLKAEEQQLPP
PLSPPSPSTPNHRRSQVIEKFEALDIEKAEHMETNAVGPSQSSDTRQGRS
EKRAFPRKRDFTNEAPPAPLPDASASPLSPHRRAKSLDRRSTEPSVTPDL
LNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRDSVAEEAADLDGEIDLS
ACYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTT
APDVTSSLPEEKNKSSCSFETCPRPTEKQEAELGEPDPEQKRSRARERRR
EGRSKTFDWAEFRPIQQALAQERVGGVGPADTHEPLRPEAEPGELERERA
RRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGLPMSDLKTHNVHVEI
EQRWHQVETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLEKELEQS
QKEASDLLEQNRLLQDQLRVALGREQSAREGYVLQATCERGFAAMEETHQ
KKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREEMERE
LEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAH
LAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGG
EATGSPLAQGKDAYELEVLLRVKESEIQYLKQEISSLKDELQTALRDKKY
ASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDSATVSGY
DIMKSKSNPDFLKKDRSCVTRQLRNIRSKSLKEGLTVQERLKLFESRDLK
KD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11147
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015134 CDS:; c980a (P327Q), delta cag 534-536 (delta S179) - both present in several ESTs and mRNAs.