cDNA Clone CHK1

• Synonyms: CHEK1
• Plasmid: pCMV5-C-Myc CHK1
• Parent Plasmid: pCMV5
• Expressed: cMyc-CHK1
• Genbank: BC004202
• Tag: Myc
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGAACAAAAACTTATTTCTGAAGAAGATCTGATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACCCTGGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAGTAACTGAAGAAGCAGTCGCAGTGAAGATTGTAGATATGAAGCGTGCCGTAGACTGTCCAGAAAATATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCATGAAAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAATATTTATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGCCAGACATAGGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTCATGGCAGGGGTGGTTTATCTGCATGGTATTGGAATAACTCACAGGGATATTAAACCAGAAAATCTTCTGTTGGATGAAAGGGATAACCTCAAAATCTCAGACTTTGGC
TTGGCAACAGTATTTCGGTATAATAATCGTGAGCGTTTGTTGAACAAGAT
GTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAGAGAAGAGAAT
TTCATGCGGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTTACTGCA
ATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGCTGTCAGGA
GTATTCTGACTGGAAAGAAAAAAAAACATACCTCAACCCTTGGAAAAAAA
TCGATTCTGCTCCTCTAGCTCTGCTGCATAAAATCTTAGTTGAGAATCCA
TCAGCAAGAATTACCATTCCAGACATCAAAAAAGATAGATGGTACAACAA
ACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCAGGTGGTGTGT
CAGAGTCTCCCAGTGGATTTTCTAAGCACATTCAATCCAATTTGGACTTC
TCTCCAGTAAACAGTGCTTCTAGTGAAGAAAATGTGAAGTACTCCAGTTC
TCAGCCAGAACCCCGCACAGGTCTTTCCTTATGGGATACCAGCCCCTCAT
ACATTGATAAATTGGTACAAGGGATCAGCTTTTCCCAGCCCACATGTCCT
GATCATATGCTTTTGAATAGTCAGTTACTTGGCACCCCAGGATCCTCACA
GAACCCCTGGCAGCGGTTGGTCAAAAGAATGACACGATTCTTTACCAAAT
TGGATGCAGACAAATCTTATCAATGCCTGAAAGAGACTTGTGAGAAGTTG
GGCTATCAATGGAAGAAAAGTTGTATGAATCAGGTTACTATATCAACAAC
TGATAGGAGAAACAATAAACTCATTTTCAAAGTGAATTTGTTAGAAATGG
ATGATAAAATATTGGTTGACTTCCGGCTTTCTAAGGGTGATGGATTGGAG
TTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTGATTGATATTGTGAG
CAGCCAGAAGGTTTGGCTTCCTGCCACATGAGTCGAC

Amino Acid Sequence

MEQKLISEEDLMAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKI
VDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSG
GELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLD
ERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPV
DVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPL
ALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSG
FSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLV
QGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKS
YQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILV
DFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKVWLPAT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11146
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with BC004202 CDS: a558g (silent); ; Estimated MW of expressed protein : 55734 Daltons