cDNA Clone slit-robo-GAP 1

• Plasmid: pEGFP-C2-Slit-Robo-GAP1 isoform 1
• Parent Plasmid: pEGFPC2
• Expressed: GFP-Slit-Robo-GAP1 isoform 1
• Genbank: NM_020762
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAATTCGAGCTCAACTGGTAGAACAACAAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCAGGATCTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCGGAATCTAGAGAAGTTAGCAGAAAGGTTCATGGCAAAAACAAGAAGCACTAAGGATCATCAACAATACAAGAAAGACCAGAACCTGTTGTCTCCAGTGAACTGCTGG
TATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGCAACCTT
GAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGATAAGTG
AGGATTCTACCAGGATGTTTAAAAAGAGCAAAGAGATTGCATTCCAACTT
CATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGGTGATGAAAAC
ATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGCTGAAAG
AGGCCGAAAAACAAGAGGAAAAGCAAATTGGGAGATCTGGTGATCCAGTC
TTCCATATTCGACTAGAGGAGAGACATCAACGGCGAAGCTCTGTAAAGAA
AATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAAATAAGC
TAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAAGCCACC
AATGCCTCAGTTTTCAAGTACTATATTCATGATCTTTCTGATTTAATTGA
TTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCCCTAAGAACAT
ATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGCTTAGAC
ATTATTGAGAATGCAGTTGATAATTTAGAGCCCAGGAGCGATAAGCAGAG
ATTCATGGAGATGTACCCTGCTGCGTTCTGTCCACCAATGAAGTTTGAGT
TTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAGCAGCCA
GTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCGCCTTGC
CACGCTCAAAATCGAGAATGAAGAGGTTAAGAAAACGACTGAAGCCACCT
TGCAGACGATACAAGATATGGTCACCATCGAGGACTATGATGTTTCTGAA
TGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGTCTCTGA
AACCTACCTGAGTAAACCCAGCATCGCCAAGAGAAGAGCCAACCAGCAGG
AAACTGAACAGTTCTACTTCATGAAACTCAGAGAATATTTGGAAGGCAGT
AATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAGGACCCT
GGGAGAAGGTCATAGAGCTGAATATATGACTACAAGGCCTCCAAATGTTC
CCCCTAAGCCCCAGAAACACAGGAAGTCCAGGCCCCGCTCACAGTATAAT
ACTAAGTTGTTTAATGGGGATTTGGAAACATTCGTCAAGGACTCAGGACA
GGTTATTCCCCTCATTGTGGAAAGCTGTATTCGGTTCATCAATCTCTATG
GTCTTCAGCATCAGGGGATTTTCAGAGTGTCTGGTTCCCAGGTGGAAGTC
AATGATATTAAAAATTCATTTGAGAGAGGTGAAAATCCTTTGGCTGATGA
CCAGAGTAACCATGATATTAACTCAGTTGCTGGCGTTCTGAAGCTCTATT
TCCGTGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGATTTAACGATCTG
ATTTCTTGTATCAGAATAGATAATCTCTATGAGAGGGCGCTTCACATCCG
CAAACTCCTCCTGACTTTGCCCAGGTCGGTCCTTATAGTGATGAGGTACC
TCTTTGCCTTCCTCAATCATCTATCACAGTACAGCGATGAGAATATGATG
GACCCTTATAACCTGGCCATTTGCTTTGGCCCAACATTGATGCCTGTCCC
AGAAATACAGGATCAAGTGTCTTGCCAGGCACATGTGAATGAAATTATCA
AAACCATCATCATCCACCATGAGACTATTTTCCCAGATGCTAAAGAGCTG
GATGGCCCTGTTTATGAGAAATGTATGGCTGGAGATGACTATTGCGACAG
CCCATACAGTGAGCACGGTACATTGGAGGAAGTGGACCAAGATGCTGGTA
CAGAGCCCCACACAAGTGAAGATGAATGTGAGCCAATAGAAGCAATAGCC
AAGTTTGACTATGTTGGGCGGTCCGCCAGAGAACTATCCTTCAAGAAGGG
TGCCTCCCTGCTGCTGTATCACCGTGCATCTGAGGACTGGTGGGAAGGCA
GGCACAACGGGATTGACGGGCTGGTGCCTCACCAGTATATAGTGGTGCAG
GATATGGATGATACGTTTTCAGACACTCTGAGCCAAAAAGCCGACAGTGA
GGCCAGCAGTGGGCCAGTCACGGAAGACAAGTCCTCATCCAAGGACATGA
ACTCCCCGACAGACCGTCATCCTGACGGCTATTTAGCCAGGCAACGAAAA
AGAGGAGAGCCACCCCCTCCAGTAAGGCGTCCTGGCAGGACCAGTGATGG
CCATTGCCCGCTCCACCCTCCACATGCCCTTTCTAACTCCTCAGTTGACC
TAGGGTCCCCAAGCCTTGCCAGTCACCCCCGGGGCCTGCTGCAGAACCGT
GGCCTCAACAATGACAGTCCTGAGCGGAGGCGCAGGCCTGGCCATGGCAG
CCTGACCAACATCAGCCGGCACGACTCCCTCAAGAAGATCGACAGCCCTC
CCATTAGAAGGTCCACGTCATCAGGGCAATACACGGGCTTCAATGACCAC
AAGCCACTGGACCCAGAGACAATTGCTCAGGATATTGAAGAAACGATGAA
CACAGCTTTGAATGAACTCCGAGAACTGGAGAGACAGAGCACAGCAAAGC
ATGCCCCTGATGTGGTGCTGGATACCCTGGAGCAAGTGAAAAACTCTCCC
ACCCCTGCCACTTCCACGGAATCTCTCAGCCCTTTGCACAACGTTGCCCT
CAGGAGCTCCGAGCCTCAGATTCGACGTAGCACGAGCTCCTCCAGTGACA
CAATGAGTACTTTCAAGCCTATGGTGGCACCCAGAATGGGCGTGCAGCTG
AAGCCTCCAGCCCTTAGGCCAAAACCTGCTGTTCTTCCAAAAACAAATCC
TACCATAGGACCTGCCCCACCTCCCCAGGGTCCAACAGACAAGTCATGCA
CAATGTAAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFMSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQD
LQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVN
CWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMFKKSKEIAF
QLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQIGRSGD
PVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLE
ATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEG
LDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQ
QPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQDMVTIEDYDV
SECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQFYFMKLREYLE
GSNLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQKHRKSRPRSQ
YNTKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQV
EVNDIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFN
DLISCIRIDNLYERALHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDEN
MMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAK
ELDGPVYEKCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEA
IAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIV
VQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRHPDGYLARQ
RKRGEPPPPVRRPGRTSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQ
NRGLNNDSPERRRRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFN
DHKPLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKN
SPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGV
QLKPPALRPKPAVLPKTNPTIGPAPPPQGPTDKSCTM*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU11103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee