cDNA Clone PTIP

• Plasmid: pGEX6P-3-PTIP
• Parent Plasmid: pGEX6P3
• Expressed: GST-PTIP
• Genbank: pGEX6P-3
• Tag: GST
• Species: Human

Sequence of Insert

gaattctTCGGACCAGGCGCCCAAAGTTCCTGAGGAGATGTTCAGGGAGG
TCAAGTATTACGCGGTGGGCGACATCGACCCGCAGGTTATTCAGCTTCTC
AAGGCTGGAAAAGCGAAGGAAGTTTCCTACAATGCACTAGCCTCACACAT
AATCTCAGAGGATGGGGACAATCCAGAGGTGGGAGAAGCTCGGGAAGTCT
TTGACTTACCTGTTGTAAAGCCTTCTTGGGTGATTCTGTCCGTTCAGTGT
GGAACTCTTCTGCCAGTAAATGGTTTTTCTCCAGAATCATGTCAGATTTT
TTTTGGAATCACTGCCTGCCTTTCTCAGGTGTCATCTGAAGACAGAAGTG
CCCTGTGGGCTTTGGTTACGTTCTATGGGGGAGATTGCCAGCTAACCCTC
AATAAGAAATGCACGCATTTGATTGTTCCAGAGCCAAAGGGGGAGAAATA
CGAATGTGCTTTAAAGCGAGCAAGTATTAAAATTGTGACTCCTGACTGGG
TTCTGGATTGCGTATCAGAGAAAACCAAAAAGGACGAAGCATTTTATCAT
CCTCGTCTGATTATTTATGAAGAGGAAGAAGAGGAAGAGGAAGAGGAGGA
GGAAGTAGAAAATGAGGAACAAGATTCTCAGAATGAGGGTAGTACAGATG
AGAAGTCAAGCCCTGCCAGCTCTCAAGAAGGGTCTCCTTCAGGTGACCAG
CAGTTTTCACCTAAATCCAACACTGAAAAATCTAAAGGGGAATTAATGTT
TGATGATTCTTCAGATTCATCACCGGAAAAACAGGAGAGAAATTTAAACT
GGACCCCGGCCGAAGTCCCACAGTTAGCTGCAGCAAAACGCAGGCTGCCT
CAGGGAAAGGAGCCTGGGTTGATTAACTTGTGTGCCAATGTCCCACCCGT
CCCAGGTAACATTTTGCCCCCTGAGGTCCGGGGTAATTTAATGGCTGCTG
GACAAAACCTCCAAAGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGT
CCAGCTGTACGGACACTGAGGAATATTACTAATAATGCTGACATTCAGCA
GATGAACCGGCCATCAAATGTAGCACATATCcTACAGACTCTcTCAGCAC
CTACGAAAAATTTAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACA
AATGCCAATGCAGTGCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACA
CATGCTACAGCAGCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCGG
TTTTACACCTTCAGCCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCAG
CAGCAGATCTCTCAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTTC
ACAGCAACAGCAGCAGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAGC
AACAGCTACAGTTTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCAT
CGCCCTCAGCAGCAGCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGCA
GCAGTTCCATCAGCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTCGCCC
AGCTCCAGCAGCAGCACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGATT
CAGCAGCAGCAGCTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGAT
GCAAAGTCAGACAGCGCCACACTTGAGTCAGACGTCACAGGCGCTGCAGC
ATCAGGTTCCACCTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCCA
CCACCATCGCCTCAGCAGCATCAGCTTTTTGGACATGATCCAGCAGTGGA
GATTCCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATT
ATCCAGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATA
ATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCAC
GCACCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAA
GAGAAAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAG
AAGAAGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTT
CCCACCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGAT
TTGTTGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGT
GCCAAATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAA
AGAACCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCT
GTGTCAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCA
CTGAGGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCC
ATTTGCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAG
TTCCCTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCC
AAACTGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGC
CAGAATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGA
CCCCTTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTAT
ATTAAGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAA
GTGCACACACCTCATTGCCAGCAAgGTGACTCGCACCGTGAAGTTCCTGA
CGGCGATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAA
TGCTTCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGA
TGCTGAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAAC
GGGCACACGTTTCTCCACTCTTTAAGGCAAAATATTTTTACATCACACCT
GGAATCTGCCCAAGTCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGG
AGGAAAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACA
AGCAGAACTCGAGTTTGTCGGAAATAATTTTAATATCCTGTGAAAATGAC
CTTCATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGC
AGAGTTCGTTCTGACTGGAGTGCTCACTCAAACGCTGGACTATGAATCAT
ATAAGTTTAACTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSSDQAPKVPEEMFREVK
YYAVGDIDPQVIQLLKAGKAKEVSYNALASHIISEDGDNPEVGEAREVFD
LPVVKPSWVILSVQCGTLLPVNGFSPESCQIFFGITACLSQVSSEDRSAL
WALVTFYGGDCQLTLNKKCTHLIVPEPKGEKYECALKRASIKIVTPDWVL
DCVSEKTKKDEAFYHPRLIIYEEEEEEEEEEEEVENEEQDSQNEGSTDEK
SSPASSQEGSPSGDQQFSPKSNTEKSKGELMFDDSSDSSPEKQERNLNWT
PAEVPQLAAAKRRLPQGKEPGLINLCANVPPVPGNILPPEVRGNLMAAGQ
NLQSSERSEMIATWSPAVRTLRNITNNADIQQMNRPSNVAHILQTLSAPT
KNLEQQVNHSQQGHTNANAVLFSQVKVTPETHMLQQQQQAQQQQQQHPVL
HLQPQQIMQLQQQQQQQISQQPYPQQPPHPFSQQQQQQQQAHPHQFSQQQ
LQFPQQQLHPPQQLHRPQQQLQPFQQQHALQQQFHQLQQHQLQQQQLAQL
QQQHSLLQQQQQQQIQQQQLQRMHQQQQQQQMQSQTAPHLSQTSQALQHQ
VPPQQPPQQQQQQQPPPSPQQHQLFGHDPAVEIPEEGFLLGCVFAIADYP
EQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCESQVSSAYAQAIRE
RKRCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGGKPCSQHIISVTGFV
DSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEKAKEWRIPCV
NAQWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPTQHLVLNLLDAWRVP
LKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLTPELTP
FVLFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHLIASKVTRTVKFLTA
ISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAEVLFSFSLEESLKRA
HVSPLFKAKYFYITPGICPSLSTMKAIVECAGGKVLSKQPSFRKLMEHKQ
NSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLDYESYK
FN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11093
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 148328 Daltons