cDNA Clone PTIP

• Plasmid: pEBG6P-1 hPTIP
• Parent Plasmid: pEBG6P
• Expressed: GST-PTIP
• Tag: GST
• Species: Human

Sequence of Insert

actagtTCGGACCAGGCGCCCAAAGTTCCTGAGGAGATGTTCAGGGAGG
TCAAGTATTACGCGGTGGGCGACATCGACCCGCAGGTTATTCAGCTTCTC
AAGGCTGGAAAAGCGAAGGAAGTTTCCTACAATGCACTAGCCTCACACAT
AATCTCAGAGGATGGGGACAATCCAGAGGTGGGAGAAGCTCGGGAAGTCT
TTGACTTACCTGTTGTAAAGCCTTCTTGGGTGATTCTGTCCGTTCAGTGT
GGAACTCTTCTGCCAGTAAATGGTTTTTCTCCAGAATCATGTCAGATTTT
TTTTGGAATCACTGCCTGCCTTTCTCAGGTGTCATCTGAAGACAGAAGTG
CCCTGTGGGCTTTGGTTACGTTCTATGGGGGAGATTGCCAGCTAACCCTC
AATAAGAAATGCACGCATTTGATTGTTCCAGAGCCAAAGGGGGAGAAATA
CGAATGTGCTTTAAAGCGAGCAAGTATTAAAATTGTGACTCCTGACTGGG
TTCTGGATTGCGTATCAGAGAAAACCAAAAAGGACGAAGCATTTTATCAT
CCTCGTCTGATTATTTATGAAGAGGAAGAAGAGGAAGAGGAAGAGGAGGA
GGAAGTAGAAAATGAGGAACAAGATTCTCAGAATGAGGGTAGTACAGATG
AGAAGTCAAGCCCTGCCAGCTCTCAAGAAGGGTCTCCTTCAGGTGACCAG
CAGTTTTCACCTAAATCCAACACTGAAAAATCTAAAGGGGAATTAATGTT
TGATGATTCTTCAGATTCATCACCGGAAAAACAGGAGAGAAATTTAAACT
GGACCCCGGCCGAAGTCCCACAGTTAGCTGCAGCAAAACGCAGGCTGCCT
CAGGGAAAGGAGCCTGGGTTGATTAACTTGTGTGCCAATGTCCCACCCGT
CCCAGGTAACATTTTGCCCCCTGAGGTCCGGGGTAATTTAATGGCTGCTG
GACAAAACCTCCAAAGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGT
CCAGCTGTACGGACACTGAGGAATATTACTAATAATGCTGACATTCAGCA
GATGAACCGGCCATCAAATGTAGCACATATCcTACAGACTCTcTCAGCAC
CTACGAAAAATTTAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACA
AATGCCAATGCAGTGCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACA
CATGCTACAGCAGCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCGG
TTTTACACCTTCAGCCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCAG
CAGCAGATCTCTCAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTTC
ACAGCAACAGCAGCAGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAGC
AACAGCTACAGTTTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCAT
CGCCCTCAGCAGCAGCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGCA
GCAGTTCCATCAGCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTCGCCC
AGCTCCAGCAGCAGCACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGATT
CAGCAGCAGCAGCTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGAT
GCAAAGTCAGACAGCGCCACACTTGAGTCAGACGTCACAGGCGCTGCAGC
ATCAGGTTCCACCTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCCA
CCACCATCGCCTCAGCAGCATCAGCTTTTTGGACATGATCCAGCAGTGGA
GATTCCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATT
ATCCAGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATA
ATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCAC
GCACCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAA
GAGAAAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAG
AAGAAGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTT
CCCACCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGAT
TTGTTGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGT
GCCAAATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAA
AGAACCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCT
GTGTCAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCA
CTGAGGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCC
ATTTGCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAG
TTCCCTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCC
AAACTGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGC
CAGAATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGA
CCCCTTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTAT
ATTAAGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAA
GTGCACACACCTCATTGCCAGCAAgGTGACTCGCACCGTGAAGTTCCTGA
CGGCGATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAA
TGCTTCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGA
TGCTGAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAAC
GGGCACACGTTTCTCCACTCTTTAAGGCAAAATATTTTTACATCACACCT
GGAATCTGCCCAAGTCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGG
AGGAAAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACA
AGCAGAACTCGAGTTTGTCGGAAATAATTTTAATATCCTGTGAAAATGAC
CTTCATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGC
AGAGTTCGTTCTGACTGGAGTGCTCACTCAAACGCTGGACTATGAATCAT
ATAAGTTTAACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSSDQAPKVPEEM
FREVKYYAVGDIDPQVIQLLKAGKAKEVSYNALASHIISEDGDNPEVGEA
REVFDLPVVKPSWVILSVQCGTLLPVNGFSPESCQIFFGITACLSQVSSE
DRSALWALVTFYGGDCQLTLNKKCTHLIVPEPKGEKYECALKRASIKIVT
PDWVLDCVSEKTKKDEAFYHPRLIIYEEEEEEEEEEEEVENEEQDSQNEG
STDEKSSPASSQEGSPSGDQQFSPKSNTEKSKGELMFDDSSDSSPEKQER
NLNWTPAEVPQLAAAKRRLPQGKEPGLINLCANVPPVPGNILPPEVRGNL
MAAGQNLQSSERSEMIATWSPAVRTLRNITNNADIQQMNRPSNVAHILQT
LSAPTKNLEQQVNHSQQGHTNANAVLFSQVKVTPETHMLQQQQQAQQQQQ
QHPVLHLQPQQIMQLQQQQQQQISQQPYPQQPPHPFSQQQQQQQQAHPHQ
FSQQQLQFPQQQLHPPQQLHRPQQQLQPFQQQHALQQQFHQLQQHQLQQQ
QLAQLQQQHSLLQQQQQQQIQQQQLQRMHQQQQQQQMQSQTAPHLSQTSQ
ALQHQVPPQQPPQQQQQQQPPPSPQQHQLFGHDPAVEIPEEGFLLGCVFA
IADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCESQVSSAYA
QAIRERKRCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGGKPCSQHIIS
VTGFVDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEKAKEW
RIPCVNAQWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPTQHLVLNLLD
AWRVPLKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLT
PELTPFVLFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHLIASKVTRTV
KFLTAISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAEVLFSFSLEE
SLKRAHVSPLFKAKYFYITPGICPSLSTMKAIVECAGGKVLSKQPSFRKL
MEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLD
YESYKFN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11091
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with PTIP N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 148811 Daltons