cDNA Clone Itch

• Plasmid: pEBG6P-1 Itch
• Parent Plasmid: pEBG6P
• Expressed: GST-Itch
• Genbank: NM_031483
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTTTGTTGGGAACTGCTGCATTA
GATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAG
ACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACC
AATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTC
CAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTG
AAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCA
TCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACG
ACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT
CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACA
AATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCC
TCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTC
AAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGA
GTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGA
ACCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTT
ATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG
GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCA
AGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATT
TATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCA
CCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAA
CCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAAT
TAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTGGAT
GGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA
TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATG
TTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAA
CTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTT
TGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAA
GACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGT
GTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAAT
GTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA
ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATT
GGCAGATTTATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGG
TTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCA
AGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTT
AAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGA
CAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTG
GCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA
GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTT
TGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATG
CAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAAT
GACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACA
AATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAA
TGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGA
TTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA
AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCC
TGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTG
TTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSDSGSQLGSMGS
LTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSP
KWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKL
EEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQN
DDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPP
RPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSG
LIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTW
DRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQR
SQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRV
YFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTT
TYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTR
KTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEV
LNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKF
IDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMY
FSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQT
QAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYAR
TSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKF
CIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11086
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Itch with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 126091 Daltons