cDNA Clone slit-robo-GAP 1

• Plasmid: pEGFP-C2-srGAP1 isoform 2
• Parent Plasmid: pEGFPC2
• Expressed: GFP-srGAP1 isoform 2
• Genbank: AB037725*
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAATTCGAGCTCAACTGGTAGAACAACAAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCAGGATCTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCGGAATCTAGAGAAGTTAGCAGAAAGGTTCATGGCAAAAACAAGAAGCACTAAGGATCATCAACAATACAAGAAAGACCAGAACCTGTTGTCTCCAGTGAACTGCTGG
TATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGCAACCTT
GAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGATAAGTG
AGGATTCTACCAGGATGTTTAAAAAGAGCAAAGAGATTGCATTCCAACTT
CATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGGTGATGAAAAC
ATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGCTGAAAG
AGGCCGAAAAACAAGAGGAAAAGCAAATTGGGAGATCTGGTGATCCAGTC
TTCCATATTCGACTAGAGGAGAGACATCAACGGCGAAGCTCTGTAAAGAA
AATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAAATAAGC
TAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAAGCCACC
AATGCCTCAGTTTTCAAGTACTATATTCATGATCTTTCTGATTTAATTGA
TTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCCCTAAGAACAT
ATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGCTTAGAC
ATTATTGAGAATGCAGTTGATAATTTAGAGCCCAGGAGCGATAAGCAGAG
ATTCATGGAGATGTACCCTGCTGCGTTCTGTCCACCAATGAAGTTTGAGT
TTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAGCAGCCA
GTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCGCCTTGC
CACGCTCAAAATCGAGAATGAAGAGGTTAAGAAAACGACTGAAGCCACCT
TGCAGACGATACAAGATATGGTCACCATCGAGGACTATGATGTTTCTGAA
TGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGTCTCTGA
AACCTACCTGAGTAAACCCAGCATCGCCAAGAGAAGAGCCAACCAGCAGG
AAACTGAACAGTTCTACTTCATGAAACTCAGAGAATATTTGGAAGGCAGT
AATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAGGACCCT
GGGAGAAGGTCATAGAGCTGAATATATGACTACAAGCCGAGGACGAAGAA
ACTCGCACACAAGACATCAGGACTCAGGACAGGTTATTCCCCTCATTGTG
GAAAGCTGTATTCGGTTCATCAATCTCTATGGTCTTCAGCATCAGGGGAT
TTTCAGAGTGTCTGGTTCCCAGGTGGAAGTCAATGATATTAAAAATTCAT
TTGAGAGAGGTGAAAATCCTTTGGCTGATGACCAGAGTAACCATGATATT
AACTCAGTTGCTGGCGTTCTGAAGCTCTATTTCCGTGGGCTGGAAAACCC
CCTCTTTCCTAAGGAAAGATTTAACGATCTGATTTCTTGTATCAGAATAG
ATAATCTCTATGAGAGGGCGCTTCACATCCGCAAACTCCTCCTGACTTTG
CCCAGGTCGGTCCTTATAGTGATGAGGTACCTCTTTGCCTTCCTCAATCA
TCTATCACAGTACAGCGATGAGAATATGATGGACCCTTATAACCTGGCCA
TTTGCTTTGGCCCAACATTGATGCCTGTCCCAGAAATACAGGATCAAGTG
TCTTGCCAGGCACATGTGAATGAAATTATCAAAACCATCATCATCCACCA
TGAGACTATTTTCCCAGATGCTAAAGAGCTGGATGGCCCTGTTTATGAGA
AATGTATGGCTGGAGATGACTATTGCGACAGCCCATACAGTGAGCACGGT
ACATTGGAGGAAGTGGACCAAGATGCTGGTACAGAGCCCCACACAAGTGA
AGATGAATGTGAGCCAATAGAAGCAATAGCCAAGTTTGACTATGTTGGGC
GGTCCGCCAGAGAACTATCCTTCAAGAAGGGTGCCTCCCTGCTGCTGTAT
CACCGTGCATCTGAGGACTGGTGGGAAGGCAGGCACAACGGGATTGACGG
GCTGGTGCCTCACCAGTATATAGTGGTGCAGGATATGGATGATACGTTTT
CAGACACTCTGAGCCAAAAAGCCGACAGTGAGGCCAGCAGTGGGCCAGTC
ACGGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACAGACCGTCA
TCCTGACGGCTATTTAGCCAGGCAACGAAAAAGAGGAGAGCCACCCCCTC
CAGTAAGGCGTCCTGGCAGGACCAGTGATGGCCATTGCCCGCTCCACCCT
CCACATGCCCTTTCTAACTCCTCAGTTGACCTAGGGTCCCCAAGCCTTGC
CAGTCACCCCCGGGGCCTGCTGCAGAACCGTGGCCTCAACAATGACAGTC
CTGAGCGGAGGCGCAGGCCTGGCCATGGCAGCCTGACCAACATCAGCCGG
CACGACTCCCTCAAGAAGATCGACAGCCCTCCCATTAGAAGGTCCACGTC
ATCAGGGCAATACACGGGCTTCAATGACCACAAGCCACTGGACCCAGAGA
CAATTGCTCAGGATATTGAAGAAACGATGAACACAGCTTTGAATGAACTC
CGAGAACTGGAGAGACAGAGCACAGCAAAGCATGCCCCTGATGTGGTGCT
GGATACCCTGGAGCAAGTGAAAAACTCTCCCACCCCTGCCACTTCCACGG
AATCTCTCAGCCCTTTGCACAACGTTGCCCTCAGGAGCTCCGAGCCTCAG
ATTCGACGTAGCACGAGCTCCTCCAGTGACACAATGAGTACTTTCAAGCC
TATGGTGGCACCCAGAATGGGCGTGCAGCTGAAGCCTCCAGCCCTTAGGC
CAAAACCTGCTGTTCTTCCAAAAACAAATCCTACCATAGGACCTGCCCCA
CCTCCCCAGGGTCCAACAGACAAGTCATGCACAATGTAAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFMSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQD
LQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVN
CWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMFKKSKEIAF
QLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQIGRSGD
PVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLE
ATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEG
LDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQ
QPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQDMVTIEDYDV
SECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQFYFMKLREYLE
GSNLITKLQAKHDLLQRTLGEGHRAEYMTTSRGRRNSHTRHQDSGQVIPL
IVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENPLADDQSNH
DINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIRKLLL
TLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQD
QVSCQAHVNEIIKTIIIHHETIFPDAKELDGPVYEKCMAGDDYCDSPYSE
HGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASLL
LYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSG
PVTEDKSSSKDMNSPTDRHPDGYLARQRKRGEPPPPVRRPGRTSDGHCPL
HPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERRRRPGHGSLTNI
SRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALN
ELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSE
PQIRRSTSSSSDTMSTFKPMVAPRMGVQLKPPALRPKPAVLPKTNPTIGP
APPPQGPTDKSCTM*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU11065
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. In this clone srGAP1 isoform 2 starts as isoform 1 (NM_020762) i.e. MSTPSRFKKDKEIIAEYESQVKEIR…