cDNA Clone TBC1D1

• Plasmid: pCMV5-HA TBC1D1
• Parent Plasmid: pCMV5
• Expressed: HA-TBC1D1
• Genbank: NM_015173
• Tag: HA
• Species: Human

Sequence of Insert

ggtaccGCCACCATGTACCCATACGATGTGCCAGATTACGCCatggaacc
aataacattcacagcaaggaaacatctgctttctaacgaggtctcggtgg
attttggcctgcagctggtgggctccctgcctgtgcattccctgaccacc
atgcccatgctgccctgggttgtggctgaggtgcgaagactcagcaggca
gtccaccagaaaggaacctgtaaccaagcaagtccggctttgcgtttcac
cctctggactgagatgtgaacctgagccagggagaagtcaacagtgggat
cccctgatctattccagcatctttgagtgcaagcctcagcgtgttcacaa
actgattcacaacagtcatgacccaagttactttgcttgtctgattaagg
aagacgctgtccaccggcagagtatctgctatgtgttcaaagccgatgat
caaacaaaagtgcctgagatcatcagctccatccgtcaggcggggaagat
cgcccggcaggaggagctgcactgcccgtccgagttcgacgacacgtttt
ccaagaagttcgaggtgctcttctgcggccgcgtgacggtggcgcacaag
aaggctccgccggccctgatcgacgagtgcatcgagaagttcaatcacgt
cagcggcagccgggggtccgagagcccccgccccaacccgccccatgccg
cgcccacagggagccaggagcctgtgcgcaggcccatgcgcaagtccttc
tcccagcccggcctgcgctcgctggcctttaggaaggagctgcaggatgg
gggcctccgaagcagcggcttcttcagctccttcgaggagagcgacattg
agaaccacctcattagcggacacaatattgtgcagcccacagatatcgag
gaaaatcgaactatgctcttcacgattggccagtctgaagtttacctcat
cagtcctgacaccaaaaaaatagcattggagaaaaattttaaggagatat
ccttttgctctcagggcatcagacacgtggaccactttgggtttatctgt
cgggagtcttccggaggtggcggctttcattttgtctgttacgtgtttca
gtgcacaaatgaggctctggttgatgaaattatgatgaccctgaaacagg
ccttcacggtggccgcagtgcagcagacagctaaggcgccagcccagctg
tgtgagggctgccccctgcaaagcctgcacaagctctgtgagaggataga
gggaatgaattcttccaaaacaaaactagaactgcaaaagcacctgacga
cattaacaaatcaggagcaggcgactatttttgaagaggttcagaaattg
agaccgagaaatgagcagcgagagaatgaattgattatttcttttctgag
atgtttatatgaagagaaacagaaagaacacatccatattggggagatga
agcagacatcgcagatggcagcagagaatattggaagtgaattaccaccc
agtgccactcgatttaggctagatatgctgaaaaacaaagcaaagagatc
tttaacagagtctttagaaagtattttgtcccggggtaataaagccagag
gcctgcaggaacactccatcagtgtggatctggatagctccctgtctagt
acattaagtaacaccagcaaagagccatctgtgtgtgaaaaggaggcctt
gcccatctctgagagctcctttaagctcctcggctcctcggaggacctgt
ccagtgactcggagagtcatctcccagaagagccagctccgctgtcgccc
cagcaggccttcaggaggcgagcaaacaccctgagtcacttccccatcga
atgccaggaacctccacaacctgcccgggggtccccgggggtttcgcaaa
ggaaacttatgaggtatcactcagtgagcacagagacgcctcatgaacga
aaggactttgaatccaaagcaaaccatcttggtgattctggtgggactcc
tgtgaagacccggaggcattcctggaggcagcagatattcctccgagtag
ccaccccgcagaaggcgtgcgattcttccagcagatatgaagattattca
gagctgggagagcttcccccacgatctcctttagaaccagtttgtgaaga
tgggccctttggccccccaccagaggaaaagaaaaggacatctcgtgagc
tccgagagctgtggcaaaaggctattcttcaacagatactgctgcttaga
atggagaaggaaaatcagaagctccaagcctctgaaaatgatttgctgaa
caagcgcctgaagctcgattatgaagaaattactccctgtcttaaagaag
taactacagtgtgggaaaagatgcttagcactccaggaagatcaaaaatt
aagtttgacatggaaaaaatgcactcggctgttgggcaaggtgtgccacg
tcatcaccgaggtgaaatctggaaatttctagctgagcaattccacctta
aacaccagtttcccagcaaacagcagccaaaggatgtgccatacaaagaa
ctcttaaagcagctgacttcccagcagcatgcgattcttattgaccttgg
gcgaacctttcctacacacccatacttctctgcccagcttggagcaggac
agctatcgctttacaacattttgaaggcctactcacttctagaccaggaa
gtgggatattgccaaggtctcagctttgtagcaggcattttgcttcttca
tatgagtgaggaagaggcgtttaaaatgctcaagtttctgatgtttgaca
tggggctgcggaaacagtatcggccagacatgattattttacagatccag
atgtaccagctctcgaggttgcttcatgattaccacagagacctctacaa
tcacctggaggagcacgagatcggccccagcctctacgctgccccctggt
tcctcaccatgtttgcctcacagttcccgctgggattcgtagccagagtc
tttgatatgatttttcttcagggaacagaggtcatatttaaagtggcttt
aagtctgttgggaagccataagcccttgattctgcagcatgaaaacctag
aaaccatagttgactttataaaaagcacgctacccaaccttggcttggta
cagatggaaaagaccatcaatcaggtatttgaaatggacatcgctaaaca
gttacaagcttatgaagttgagtaccacgtccttcaagaagaacttatcg
attcctctcctctcagtgacaaccaaagaatggataaattagagaaaacc
aacagcagcttacgcaaacagaaccttgacctccttgaacagttgcaggt
ggcaaatggtaggatccaaagccttgaggccaccattgagaagctcctga
gcagtgagagcaagctgaagcaggccatgcttaccttagaactggagcgg
tcggccctgctgcagacggtggaggagctgcggcggcggagcgcagagcc
cagcgaccgggagcctgagtgcacgcagcccgagcccacgggcgactgag
tcgac

Amino Acid Sequence

MYPYDVPDYAMEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPML
PWVVAEVRRLSRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIY
SSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKV
PEIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPP
ALIDECIEKFNHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPG
LRSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRT
MLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESS
GGGGFHFVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGC
PLQSLHKLCERIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRN
EQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATR
FRLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSN
TSKEPSVCEKEALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAF
RRRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFE
SKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGE
LPPRSPLEPVCEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKE
NQKLQASENDLLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDM
EKMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQ
LTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYC
QGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQL
SRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMI
FLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEK
TINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSL
RKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALL
QTVEELRRRSAEPSDREPECTQPEPTGD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11049
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422); ; Derivatives:; DU 11050 = pCMV5 HA-TBC1D1 T596A; DU 11051 = pCMV5 HA-TBC1D1 F1046A; ; Estimated MW of expressed protein : 134309 Daltons