cDNA Clone MELK

• Plasmid: pGEX6P-1-HA-MELK kinase domain
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA-MELK kinase domain
• Genbank: NM_014791
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ggatccTACCCATACGATGTGCCAGATTACGCCAAAGATTATGATGAACT
TCTCAAATATTATGAATTACATGAAACTATTGGGACAGGTGGCTTTGCAA
AGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAA
ATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGA
GATTGAGGCCTTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACC
ATGTGCTAGAGACAGCCAACAAAATATTCATGGTTCTTGAGTACTGCCCT
GGAGGAGAGCTGTTTGACTATATAATTTCCCAGGATCGCCTGTCAGAAGA
GGAGACCCGGGTTGTCTTCCGTCAGATAGTATCTGCTGTTGCTTATGTGC
ACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAATTTGCTGTTT
GATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACC
CAAGGGTAACAAGGATTACCATCTACAGACATGCTGTGGGAGTCTGGCTT
ATGCAGCACCTGAGTTAATACAAGGCAAATCATATCTTGGATCAGAGGCA
GATGTTTGGAGCATGGGCATACTGTTATATGTTCTTATGTGTGGATTTCT
ACCATTTGATGATGATAATGTAATGGCTTTATACAAGAAGATTATGAGAG
GAAAATATGATGTTCCCAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTT
CAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCT
ATTGAACCATCCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGC
AAAGCAAGAATCCTTTTATTCACCTCGATGATGATTGCGTAACAGAACTT
TCTGTACATCACAGAAACAACAGGCAAACAATGGAGGATTTAATTTCACT
GTGGCAGTATGATCACCTCACGGCTACCTATTAAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSYPYDVPDYAKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELSVHHRNNRQTMEDLISLWQYDHLTATY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1104
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express MELK kinase domain with N terminal GST and HA tags