cDNA Clone MELK

• Plasmid: pEBG-2T-HA MELK
• Parent Plasmid: pEBG2T
• Expressed: GST-HA-MELK
• Genbank: NM_014791
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ggatccTACCCATACGATGTGCCAGATTACGCCAAAGATTATGATGAACT
TCTCAAATATTATGAATTACATGAAACTATTGGGACAGGTGGCTTTGCAA
AGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAA
ATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGA
GATTGAGGCCTTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACC
ATGTGCTAGAGACAGCCAACAAAATATTCATGGTTCTTGAGTACTGCCCT
GGAGGAGAGCTGTTTGACTATATAATTTCCCAGGATCGCCTGTCAGAAGA
GGAGACCCGGGTTGTCTTCCGTCAGATAGTATCTGCTGTTGCTTATGTGC
ACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAATTTGCTGTTT
GATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACC
CAAGGGTAACAAGGATTACCATCTACAGACATGCTGTGGGAGTCTGGCTT
ATGCAGCACCTGAGTTAATACAAGGCAAATCATATCTTGGATCAGAGGCA
GATGTTTGGAGCATGGGCATACTGTTATATGTTCTTATGTGTGGATTTCT
ACCATTTGATGATGATAATGTAATGGCTTTATACAAGAAGATTATGAGAG
GAAAATATGATGTTCCCAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTT
CAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCT
ATTGAACCATCCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGC
AAAGCAAGAATCCTTTTATTCACCTCGATGATGATTGCGTAACAGAACTT
TCTGTACATCACAGAAACAACAGGCAAACAATGGAGGATTTAATTTCACT
GTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTTCTAGCCAAGA
AGGCTCGGGGAAAACCAGTTCGTTTAAGGCTTTCTTCTTTCTCCTGTGGA
CAAGCCAGTGCTACCCCATTCACAGACATCAAGTCAAATAATTGGAGTCT
GGAAGATGTGACCGCAAGTGATAAAAATTATGTGGCGGGATTAATAGACT
ATGATTGGTGTGAAGATGATTTATCAACAGGTGCTGCTACTCCCCGAACA
TCACAGTTTACCAAGTACTGGACAGAATCAAATGGGGTGGAATCTAAATC
ATTAACTCCAGCCTTATGCAGAACACCTGCAAATAAATTAAAGAACAAAG
AAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAGTACTTTATG
TTTCCTGAGCCAAAGACTCCAGTTAATAAGAACCAGCATAAGAGAGAAAT
ACTCACTACGCCAAATCGTTACACTACACCCTCAAAAGCTAGAAACCAGT
GCCTGAAAGAAACTCCAATTAAAATACCAGTAAATTCAACAGGAACAGAC
AAGTTAATGACAGGTGTCATTAGCCCTGAGAGGCGGTGCCGCTCAGTGGA
ATTGGATCTCAACCAAGCACATATGGAGGAGACTCCAAAAAGAAAGGGAG
CCAAAGTGTTTGGGAGCCTTGAAAGGGGGTTGGATAAGGTTATCACTGTG
CTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCCCAGAAGACT
AAAGCTTCACTATAATGTGACTACAACTAGATTAGTGAATCCAGATCAAC
TGTTGAATGAAATAATGTCTATTCTTCCAAAGAAGCATGTTGACTTTGTA
CAAAAGGGTTATACACTGAAGTGTCAAACACAGTCAGATTTTGGGAAAGT
GACAATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAACCCGATGTGG
TGGGTATCAGGAGGCAGCGGCTTAAGGGCGATGCCTGGGTTTACAAAAGA
TTAGTGGAAGACATCCTATCTAGCTGCAAGGTATAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSYPYDVPDYAKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGNKDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNLLNHPWIMQDYNYPVEWQSKNPFIHLDDDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFTDIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWTESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPVNKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVISPERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGSARDGPRRLKLHYNVTTTRLVNPDQLLNEIMSILPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1101
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express MELK with N terminal GST and HA tags