cDNA Clone SIK

• Plasmid: pGEX6P-1-HA-SIK kinase domain (2-309)
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA-SIK kinase domain (2-309)
• Genbank: NM_173354
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ggatccTACCCATACGATGTGCCAGATTACGCCGTTATCATGTCGGAGTT
CAGCGCGGACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAGCCCCTCCGGG
TGGGTTTTTACGACATCGAGCGGACCCTGGGCAAAGGCAACTTCGCGGTG
GTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAAAAAT
AATTGATAAAACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTG
AGGTTCAGCTGATGAAGCTTCTGAACCATCCACACATCATAAAGCTTTAC
CAGGTTATGGAAACAAAGGACATGCTTTACATCGTCACTGAATTTGCTAA
AAATGGAGAAATGTTTGATTATTTGACTTCCAACGGGCACCTGAGTGAGA
ACGAGGCGCGGAAGAAGTTCTGGCAAATCCTGTCGGCCGTGGAGTACTGT
CACGACCATCACATCGTCCACCGGGACCTCAAGACCGAGAACCTCCTGCT
GGATGGCAACATGGACATCAAGCTGGCAGATTTTGGATTTGGGAATTTCT
ACAAGTCAGGAGAGCCTCTGTCCACGTGGTGTGGGAGCCCCCCGTATGCC
GCCCCGGAAGTCTTTGAGGGGAAGGAGTATGAAGGCCCCCAGCTGGACAT
CTGGAGCCTGGGCGTGGTGCTGTACGTCCTGGTCTGCGGTTCTCTCCCCT
TCGATGGGCCTAACCTGCCGACGCTGAGACAGCGGGTGCTGGAGGGCCGC
TTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGAGCCTGATCCGCCG
CATGCTGGTGGTGGACCCCGCCAGGCGCATCACCATCGCCCAGATCCGGC
AGCACCGGTGGATGCGGGCTGAGCCCTGCTTGCCGGGACCCGCCTGCCCC
GCCTTCTCCGCACACAGCTACACCTCCAACCTGGGCGACTACGATGAGCA
GGCGCTGTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSYPYDVPDYAVIMSEFSADPAGQGQGQQKPLRVGFYDIERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPHIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYCHDHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKSGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCESLIRRMLVVDPARRITIAQIRQHRWMRAEPCLPGPACPAFSAHSYTSNLGDYDEQAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1098
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express SIK kinase domain with N terminal GST and HA tags