cDNA Clone CAK1 (yeast)

• Synonyms: CDK7
• Plasmid: pGEX6P-1-CAK1 (c-term his)
• Parent Plasmid: pGEX6P1
• Expressed: GST-CAK1 (c-term His)
• Genbank: U60192
• Tag: GST
• Species: Yeast

Sequence of Insert

ggatccAAACTGGATAGTATAGACATTACACACTGTCAGTTGGTCAAATC
TACTAGAACTGCTAGGATTTATAGGTCGGATACATATGCCATTAAATGTC
TAGCACTAGATTTCGATATCCCGCCACATAACGCCAAATTCGAAGTATCG
ATATTAAACAAACTGGGCAACAAATGTAAGCACATCTTACCTCTTCTAGA
GTCTAAGGCTACCGATAATAATGACCTATTGTTGTTGTTTCCCTTTGAAG
AGATGAACCTTTATGAGTTCATGCAAATGCACTATAAAAGAGATAGAAGA
AAAAAAAATCCCTATTACGATTTGCTAAATCCCAGTATCCCAATTGTTGC
GGACCCCCCCGTTCAGAAATATACTAATCAATTGGACGTCAATCGGTATT
CTTTGTCCTTTTTCCGGCAAATGGTTGAAGGGATTGCATTCTTACATGAG
AACAAGATCATTCACCGCGACATCAAACCGCAAAATATCATGCTAACAAA
CAATACCAGCACCGTATCCCCAAAGTTGTACATAATTGATTTTGGCATCT
CTTATGACATGGCAAATAACTCACAAACAAGTGCGGAACCCATGGATAGC
AAGGTGACGGATATAAGCACAGGAATTTACAAGGCCCCAGAAGTGCTTTT
TGGAGTAAAATGCTATGATGGTGGCGTGGACGTGTGGTCGTTGTTGATAA
TTATTTCTCAGTGGTTCCAGAGAGAAACAAGCCGTATGGGGCACGTTCCG
GCCATGATTGATGACGGCAGCGACGACATGAACTCAGATGGAAGCGATTT
CAGACTGATTTGCTCAATATTCGAAAAGTTGGGCATACCGTCCATTCAGA
AATGGGAAGAGGTTGCGCAACACGGCTCGGTTGATGCATTTGTTGGTATG
TTTGGTGCAGATGGCGATGGCAAGTATGTACTGGACCAGGAGAAAGATGT
ACAGATTAGCATTGTTGAGAGGAATATGCCTCGACTGGACGAGATTGCGG
ATGTCAAAGTCAAGCAGAAGTTCATTAATTGTATCCTGGGGATGGTTTCA
TTTTCACCAAACGAAAGATGGAGCTGTCAAAGAATCTTGCAAGAATTAGA
AAAGCCAGGTTCACACCACCACCACCACCACTAAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSKLDSIDITHCQLVKSTRTARIYRSDTYAIKCLALDFDIPPHNAKFEVSILNKLGNKCKHILPLLESKATDNNDLLLLFPFEEMNLYEFMQMHYKRDRRKKNPYYDLLNPSIPIVADPPVQKYTNQLDVNRYSLSFFRQMVEGIAFLHENKIIHRDIKPQNIMLTNNTSTVSPKLYIIDFGISYDMANNSQTSAEPMDSKVTDISTGIYKAPEVLFGVKCYDGGVDVWSLLIIISQWFQRETSRMGHVPAMIDDGSDDMNSDGSDFRLICSIFEKLGIPSIQKWEEVAQHGSVDAFVGMFGADGDGKYVLDQEKDVQISIVERNMPRLDEIADVKVKQKFINCILGMVSFSPNERWSCQRILQELEKPGSHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1089
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion with C-terminal 6His tag