cDNA Clone SIK2

• Synonyms: SIK2
• Plasmid: pEBG-2T-HA QIK
• Parent Plasmid: pEBG2T
• Expressed: GST-HA-QIK
• Genbank: XM_041314
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

actagtTACCCATACGATGTGCCAGATTACGCCGTCATGGCGGATGGCCC
GAGGCACTTGCAGCGCGGGCCGGTCCGGGTGGGGTTCTACGACATCGAGG
GCACGCTGGGCAAGGGCAACTTCGCTGTGGTGAAGCTGGGGCGGCACCGG
ATCACCAAGACGGAGGTGGCAATAAAAATAATCGATAAGTCTCAGCTGGA
TGCAGTGAACCTTGAGAAAATCTACCGAGAAGTACAAATAATGAAAATGT
TAGACCACCCTCACATAATCAAACTTTATCAGGTAATGGAGACCAAAAGT
ATGTTGTACCTTGTGACAGAATATGCCAAAAATGGAGAAATTTTTGACTA
TCTTGCTAATCATGGCCGGTTAAATGAGTCTGAAGCCAGGCGAAAATTCT
GGCAAATCCTGTCTGCTGTTGATTATTGTCATGGTCGGAAGATTGTGCAC
CGTGACCTCAAAGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAA
AATAGCAGATTTCGGTTTTGGAAATTTCTTTAAAAGTGGTGAACTGCTGG
CAACATGGTGTGGCAGCCCCCCTTATGCAGCCCCAGAAGTCTTTGAAGGG
CAGCAGTATGAAGGACCACAGCTGGACATCTGGAGTATGGGAGTTGTTCT
TTATGTCCTTGTCTGTGGAGCTCTGCCCTTTGATGGACCGACTCTTCCAA
TTTTGAGGCAGAGGGTTCTGGAAGGAAGATTCCGGATTCCGTATTTCATG
TCAGAAGATTGCGAGCACCTTATCCGAAGGATGTTGGTCCTAGACCCATC
CAAACGGCTAACCATAGCCCAAATCAAGGAGCATAAATGGATGCTCATAG
AAGTTCCTGTCCAGAGACCTGTTCTCTATCCACAAGAGCAAGAAAATGAG
CCATCCATCGGGGAGTTTAATGAGCAGGTTCTGCGACTGATGCACAGCCT
TGGAATAGATCAGCAGAAAACCATTGAGTCTTTGCAGAACAAGAGCTATA
ACCACTTTGCTGCCATTTATTTCTTGTTGGTGGAGCGCCTGAAATCACAT
CGGAGCAGTTTCCCAGTGGAGCAGAGACTTGATGGCCGCCAGCGTCGGCC
TAGCACCATTGCTGAGCAAACAGTTGCCAAGGCACAGACTGTGGGGCTCC
CAGTGACCATGCATTCACCGAACATGAGGCTGCTGCGATCTGCCCTCCTC
CCCCAGGCATCCAACGTGGAGGCCTTTTCATTTCCAGCATCTGGCTGTCA
GGCGGAAGCTGCATTCATGGAAGAAGAGTGTGTGGACACTCCAAAGGTCA
ATGGCTGTCTGCTTGACCCTGTGCCTCCTGTCCTGGTGCGGAAGGGATGC
CAGTCACTGCCCAGCAACATGATGGAGACCTCCATTGACGAAGGGCTGGA
GACAGAAGGAGAGGCCGAGGAAGACCCCGCTCATGCCTTTGAGGCATTTC
AGTCCACACGCAGCGGGCAGAGACGGCACACTCTGTCAGAAGTGACCAAT
CAACTGGTCGTGATGCCTGGGGCAGGGAAAATTTTCTCCATGAATGACAG
CCCCTCCCTTGACAGTGTGGACTCTGAGTATGATATGGGGTCTGTTCAGA
GGGACCTGAACTTTCTGGAAGACAACCCTTCCCTTAAGGACATCATGTTA
GCCAATCAGCCTTCACCCCGCATGACATCTCCCTTCATAAGCCTGAGACC
TACCAACCCAGCCATGCAGGCTCTGAGCTCCCAGAAACGAGAGGTCCACA
ACAGGTCTCCAGTGAGCTTCAGAGAGGGCCGCAGAGCATCAGATACCTCC
CTCACCCAGGGAATTGTAGCATTTAGACAACATCTTCAGAATCTGGCTAG
AACCAAAGGAATTCTAGAGTTGAACAAAGTGCAGTTGTTGTATGAACAAA
TAGGACCGGAGGCAGACCCTAACCTGGCGCCGGCGGCTCCTCAGCTCCAG
GACCTTGCTAGCAGCTGCCCTCAGGAAGAAGTTTCTCAGCAGCAGGAAAG
CGTCTCCACTCTCCCTGCCAGCGTGCATCCCCAGCTGTCCCCACGGCAGA
GCCTGGAGACCCAGTACCTGCAGCACAGACTCCAGAAGCCCAGCCTTCTG
TCAAAGGCCCAGAACACCTGTCAGCTTTATTGCAAAGAACCACCGCGGAG
CCTTGAGCAGCAGCTGCAGGAACATAGGCTCCAGCAGAAGCGACTCTTTC
TTCAGAAGCAGTCTCAACTGCAGGCCTATTTTAATCAGATGCAGATAGCA
GAGAGCTCCTACCCACAGCCAAGTCAGCAGCTGCCCCTTCCCCGCCAGGA
GACTCCACCGCCTTCTCAGCAGGCCCCACCGTTCAGCCTGACCCAGCCCC
TGAGCCCCGTCCTGGAGCCTTCCTCCGAGCAGATGCAATACAGCCCTTTC
CTCAGCCAGTACCAAGAGATGCAGCTTCAGCCCCTGCCCTCCACTTCCGG
TCCCCGGGCTGCTCCTCCTCTGCCCACGCAGCTACAGCAGCAGCAGCCGC
CACCGCCACCACCCCCTCCACCACCACGACAGCCAGGAGCTGCCCCAGCC
CCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCGCTGCTTCCCC
TGCGCCAGACTATCCCACTCCCTGTCAGTATCCTGTGGATGGAGCCCAGC
AGAGCGACCTAACGGGGCCAGACTGTCCCAGAAGCCCAGGACTGCAAGAG
GCCCCCTCCAGCTACGACCCACTAGCCCTCTCTGAGCTACCTGGACTCTT
TGATTGTGAAATGCTAGACGCTGTGGATCCACAACACAACGGGTATGTCC
TGGTGAATTAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSYPYDVPDYAVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1078
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express QIK with N terminal GST and HA tags