cDNA Clone Max

• Plasmid: pFastBac.HTb Max
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Max
• Genbank: M64240
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCAGCGATAACGATGACATCGAGGTGGAGAGCGACGAAGAGCAACC
GAGGTTTCAATCTGCGGCTGACAAACGGGCTCATCATAATGCACTGGAAC
GAAAACGTAGGGACCACATCAAAGACAGCTTTCACAGTTTGCGGGACTCA
GTCCCATCACTCCAAGGAGAGAAGGCATCCCGGGCCCAAATCCTAGACAA
AGCCACAGAATATATCCAGTATATGCGAAGGAAAAACCACACACACCAGC
AAGATATTGACGACCTCAAGCGGCAGAATGCTCTTCTGGAGCAGCAAGTC
CGTGCACTGGAGAAGGCGAGGTCAAGTGCCCAACTGCAGACCAACTACCC
CTCCTCAGACAACAGCCTCTACACCAACGCCAAGGGCAGCACCATCTCTG
CCTTCGATGGGGGCTCGGACTCCAGCTCGGAGTCTGAGCCTGAAGAGCCC
CAAAGCAGGAAGAAGCTCCGGATGGAGGCCAGCTAAGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1062
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; Changes to M64240 ORF sequence: ; g207a (silent in E69), a414g (silent in S138), a426g (silent in S142).