cDNA Clone Mcm2

• Plasmid: pGEX6P-1-Mcm2
• Parent Plasmid: pGEX6P1
• Expressed: GST-Mcm2
• Genbank: D83987
• Tag: GST
• Species: Human

Sequence of Insert

ggatccgcatccagcccggcccagcgtcggcgaggcaatgatcctctcac
ctccagccctggccgaagctcccggcgtactgatgccctcacctccagcc
ctggccgtgaccttccaccatttgaggatgagtccgaggggctcctaggc
acagaggggcccctggaggaagaagaggatggagaggagctcattggaga
tggcatggaaagggactaccgcgccatcccagagctggacgcctatgagg
ccgagggactggctctggatgatgaggacgtagaggagctgacggccagt
cagagggaggcagcagagcgggccatgcggcagcgtgaccgggaggctgg
ccggggcctgggccgcatgcgccgtgggctcctgtatgacagcgatgagg
aggacgaggagcgccctgcccgcaagcgccgccaggtggagcgggccacg
gaggacggcgaggaggacgaggagatgatcgagagcatcgagaacctgga
ggatctcaaaggccactctgtgcgcgagtgggtgagcatggcgggccccc
ggctggagatccaccaccgcttcaagaacttcctgcgcactcacgtcgac
agccacggccacaacgtcttcaaggagcgcatcagcgacatgtgcaaaga
gaaccgtgagagcctggtggtgaactatgaggacttggcagccagggagc
acgtgctggcctacttcctgcctgaggcaccggcggagctgctgcagatc
tttgatgaggctgccctggaggtggtactggccatgtaccccaagtacga
ccgcatcaccaaccacatccatgtccgcatctcccacctgcctctggtgg
aggagctgcgctcgctgaggcagctgcatctgaaccagctgatccgcacc
agtggggtggtgaccagctgcactggcgtcctgccccagctcagcatggt
caagtacaactgcaacaagtgcaatttcgtcctgggtcctttctgccagt
cccagaaccaggaggtgaaaccaggctcctgtcctgagtgccagtcggcc
ggcccctttgaggtcaacatggaggagaccatctatcagaactaccagcg
tatccgaatccaggagagtccaggcaaagtggcggctggccggctgcccc
gctccaaggacgccattctcctcgcagatctggtggacagctgcaagcca
ggagacgagatagagctgactggcatctatcacaacaactatgatggctc
cctcaacactgccaatggcttccctgtctttgccactgtcatcctagcca
accacgtggccaagaaggacaacaaggttgctgtaggggaactgaccgat
gaagatgtgaagatgatcactagcctctccaaggatcagcagatcggaga
gaagatctttgccagcattgctccttccatctatggtcatgaagacatca
agagaggcctggctctggccctgttcggaggggagcccaaaaacccaggt
ggcaagcacaaggtacgtggtgatatcaacgtgctcttgtgcggagaccc
tggcacagcgaagtcgcagtttctcaagtatattgagaaagtgtccagcc
gagccatcttcaccactggccagggggcgtcggctgtgggcctcacggcg
tatgtccagcggcaccctgtcagcagggagtggaccttggaggctggggc
cctggttctggctgaccgaggagtgtgtctcattgatgaatttgacaaga
tgaatgaccaggacagaaccagcatccatgaggccatggagcaacagagc
atctccatctcgaaggctggcatcgtcacctccctgcaggctcgctgcac
ggtcattgctgccgccaaccccataggagggcgctacgacccctcgctga
ctttctctgagaacgtggacctcacagagcccatcatctcacgctttgac
atcctgtgtgtggtgagggacaccgtggacccagtccaggacgagatgct
ggcccgcttcgtggtgggcagccacgtcagacaccaccccagcaacaagg
aggaggaggggctggccaatggcagcgctgctgagcccgccatgcccaac
acgtatggcgtggagcccctgccccaggaggtcctgaagaagtacatcat
ctacgccaaggagagggtccacccgaagctcaaccagatggaccaggaca
aggtggccaagatgtacagtgacctgaggaaagaatctatggcgacaggc
agcatccccattacggtgcggcacatcgagtccatgatccgcatggcgga
ggcccacgcgcgcatccatctgcgggactatgtgatcgaagacgacgtca
acatggccatccgcgtgatgctggagagcttcatagacacacagaagttc
agcgtcatgcgcagcatgcgcaagacttttgcccgctacctttcattccg
gcgtgacaacaatgagctgttgctcttcatactgaagcagttagtggcag
agcaggtgacatatcagcgcaaccgctttggggcccagcaggacactatt
gaggtccctgagaaggacttggtggataaggctcgtcagatcaacatcca
caacctctctgcattttatgacagtgagctcttcaggatgaacaagttca
gccacgacctgaaaaggaaaatgatcctgcagcagttctgagaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1046
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; Changes to published sequence : ; t477c (silent; I159), c1194g (N398K), g1195c (A399P); All of the above present in all available EST sequences.