cDNA Clone CAK1 (yeast)

• Synonyms: CDK7
• Plasmid: pETBlue2 CAK1-6His
• Parent Plasmid: pETBlue2 original
• Expressed: CAK1-6His
• Genbank: U60192
• Tag: 6His
• Species: Yeast

Sequence of Insert

ggacccAAACTGGATAGTATAGACATTACACACTGTCAGTTGGTCAAATC
TACTAGAACTGCTAGGATTTATAGGTCGGATACATATGCCATTAAATGTC
TAGCACTAGATTTCGATATCCCGCCACATAACGCCAAATTCGAAGTATCG
ATATTAAACAAACTGGGCAACAAATGTAAGCACATCTTACCTCTTCTAGA
GTCTAAGGCTACCGATAATAATGACCTATTGTTGTTGTTTCCCTTTGAAG
AGATGAACCTTTATGAGTTCATGCAAATGCACTATAAAAGAGATAGAAGA
AAAAAAAATCCCTATTACGATTTGCTAAATCCCAGTATCCCAATTGTTGC
GGACCCCCCCGTTCAGAAATATACTAATCAATTGGACGTCAATCGGTATT
CTTTGTCCTTTTTCCGGCAAATGGTTGAAGGGATTGCATTCTTACATGAG
AACAAGATCATTCACCGCGACATCAAACCGCAAAATATCATGCTAACAAA
CAATACCAGCACCGTATCCCCAAAGTTGTACATAATTGATTTTGGCATCT
CTTATGACATGGCAAATAACTCACAAACAAGTGCGGAACCCATGGATAGC
AAGGTGACGGATATAAGCACAGGAATTTACAAGGCCCCAGAAGTGCTTTT
TGGAGTAAAATGCTATGATGGTGGCGTGGACGTGTGGTCGTTGTTGATAA
TTATTTCTCAGTGGTTCCAGAGAGAAACAAGCCGTATGGGGCACGTTCCG
GCCATGATTGATGACGGCAGCGACGACATGAACTCAGATGGAAGCGATTT
CAGACTGATTTGCTCAATATTCGAAAAGTTGGGCATACCGTCCATTCAGA
AATGGGAAGAGGTTGCGCAACACGGCTCGGTTGATGCATTTGTTGGTATG
TTTGGTGCAGATGGCGATGGCAAGTATGTACTGGACCAGGAGAAAGATGT
ACAGATTAGCATTGTTGAGAGGAATATGCCTCGACTGGACGAGATTGCGG
ATGTCAAAGTCAAGCAGAAGTTCATTAATTGTATCCTGGGGATGGTTTCA
TTTTCACCAAACGAAAGATGGAGCTGTCAAAGAATCTTGCAAGAATTAGA
AAAGCCActcgag

Amino Acid Sequence

MAISRELVDPKLDSIDITHCQLVKSTRTARIYRSDTYAIKCLALDFDIPP
HNAKFEVSILNKLGNKCKHILPLLESKATDNNDLLLLFPFEEMNLYEFMQ
MHYKRDRRKKNPYYDLLNPSIPIVADPPVQKYTNQLDVNRYSLSFFRQMV
EGIAFLHENKIIHRDIKPQNIMLTNNTSTVSPKLYIIDFGISYDMANNSQ
TSAEPMDSKVTDISTGIYKAPEVLFGVKCYDGGVDVWSLLIIISQWFQRE
TSRMGHVPAMIDDGSDDMNSDGSDFRLICSIFEKLGIPSIQKWEEVAQHG
SVDAFVGMFGADGDGKYVLDQEKDVQISIVERNMPRLDEIADVKVKQKFI
NCILGMVSFSPNERWSCQRILQELEKPHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1044
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express 6His C-terminal fusion