cDNA Clone IkB-beta

• Plasmid: pGEX6P-1-IkB-beta
• Parent Plasmid: pGEX6P1
• Expressed: GST-IkB-beta
• Genbank: E27842
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGCTGGGGTCGCGTGCTTGGGAAAAGCTGCCGACGCAGATGAATG
GTGCGACAGCGGCCTGGGCTCCCTGGGTCCGGACGCAGCGGCCCCCGGAG
GACCTGGGTTGGGCGCGGAGTTGGGCCCGGGGCTGTCGTGGGCTCCCCTC
GTCTTCGGCTACGTCACTGAGGATGGGGACACGGCACTGCACTTGGCTGT
GATTCATCAGCATGAACCCTTCCTGGATTTTCTTCTAGGCTTCTCGGCCG
GCACTGAGTACATGGACCTGCAGAATGACCTAGGCCAGACAGCCCTGCAC
CTGGCAGCCATCCTGGGGGAGACATCCACGGTGGAGAAGCTGTACGCAGC
AGGCGCCGGGCTGTGTGTGGCGGAGCGTAGGGGCCACACGGCGCTGCACC
TGGCCTGCCGTGTGGGGGCACACGCCTGTGCCCGTGCCCTGCTTCAGCCC
CGCCCCCGGCGCCCCAGGGAAGCCCCCGACACCTACCTCGCTCAGGGCCC
TGACCGTACTCCCGACACCAACCATACCCCTGTCGCCTTGTACCCCGATT
CCGACTTGGAGAAGGAAGAAGAGGAGAGTGAGGAGGACTGGAAGCTGCAG
CTGGAGGCTGAAAACTACGAGGGCCACACCCCACTCCACGTGGCCGTTAT
CCACAAAGATGTGGAGATGGTCCGGCTGCTCCGAGATGCTGGAGCTGACC
TTGACAAACCGGAGCCCACGTGCGGCCGGAGCCCCCTTCATTTGGCAGTG
GAGGCCCAGGCAGCCGATGTGCTGGAGCTTCTCCTGAGGGCAGGCGCGAA
CCCTGCTGCCCGCATGTACGGTGGCCGCACCCCACTCGGCAGTGCCATGC
TCCGGCCCAACCCCATCCTCGCCCGCCTCCTCCGTGCACACGGAGCCCCT
GAGCCCGAGGGCGAGGACGAGAAATCCGGCCCCTGCAGCAGCAGTAGCGA
CAGCGACAGCGGAGACGAGGGCGATGAATACGACGACATTGTGGTTCACA
GCAGCCGCAGCCAAACCCGGCTGCCTCCCACCCCAGCCTCAAAACCTCTT
CCTGACGACCCCCGCCCCGTGTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1008
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion