cDNA Clone IkB-alpha

• Plasmid: pGEX6P-1-IkB-alpha
• Parent Plasmid: pGEX6P1
• Expressed: GST-IkB-alpha
• Genbank: BC004983
• Tag: GST
• Species: Human

Sequence of Insert

ggatccTTCCAGGCGGCCGAGCGCCCCCAGGAGTGGGCCATGGAGGGCCC
CCGCGACGGGCTGAAGAAGGAGCGGCTACTGGACGACCGCCACGACAGCG
GCCTGGACTCCATGAAAGACGAGGAGTACGAGCAGATGGTCAAGGAGCTG
CAGGAGATCCGCCTCGAGCCGCAGGAGGTGCCGCGCGGCTCGGAGCCCTG
GAAGCAGCAGCTCACCGAGGACGGGGACTCGTTCCTGCACTTGGCCATCA
TCCATGAAGAAAAGGCACTGACCATGGAAGTGATCCGCCAGGTGAAGGGA
GACCTGGCCTTCCTCAACTTCCAGAACAACCTGCAGCAGACTCCACTCCA
CTTGGCTGTGATCACCAACCAGCCAGAAATTGCTGAGGCACTTCTGGGAG
CTGGCTGTGATCCTGAGCTCCGAGACTTTCGAGGAAATACCCCCCTACAC
CTTGCCTGTGAGCAGGGCTGCCTGGCCAGCGTGGGAGTCCTGACTCAGTC
CTGCACCACCCCGCACCTCCACTCCATCCTGAAGGCTACCAACTACAATG
GCCACACGTGTCTACACTTAGCCTCTATCCATGGCTACCTGGGCATCGTG
GAGCTTTTGGTGTCCTTGGGTGCTGATGTCAATGCTCAGGAGCCCTGTAA
TGGCCGGACTGCCCTTCACCTCGCAGTGGACCTGCAAAATCCTGACCTGG
TGTCACTCCTGTTGAAGTGTGGGGCTGATGTCAACAGAGTTACCTACCAG
GGCTATTCTCCCTACCAGCTCACCTGGGGCCGCCCAAGCACCCGGATACA
GCAGCAGCTGGGCCAGCTGACACTAGAAAACCTTCAGATGCTGCCAGAGA
GTGAGGATGAGGAGAGCTATGACACAGAGTCAGAGTTCACGGAGTTCACA
GAGGACGAGCTGCCCTATGATGACTGTGTGTTTGGAGGCCAGCGTCTGAC
GTTATGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1007
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion