cDNA Clone DBN1

• Synonyms: drebrin 1, D0S117E
• Plasmid: pGEX6P DBN1
• Parent Plasmid: pGEX6P1
• Expressed: GST-DBN1
• Genbank: NM_004395.3 GI:166362725 with silent change g318a and coding change a1336g (I446V) - EST-derived and present in other published sequences for DBN1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCGGCGTCAGCTTCAGCGGCCACCGCCTGGAGCTGCTGGCGGCTTACGAGGAGGTGATCCGAGAGGAGAGCGCGGCCGACTGGGCTCTGTACACATATGAAGATGGCTCCGATGACCTCAAGCTTGCAGCATCAGGAGAAGGGGGCTTGCAGGAGCTTTCGGGACACTTTGAGAACCAGAAGGTGATGTACGGCTTCTGCAGTGTCAAGGACTCCCAAGCTGCTCTGCCAAAATACGTGCTCATCAACTGGGTGGGCGAAGATGTGCCTGATGCCCGCAAGTGCGCTTGTGCCAGCCACGTGGCTAAGGTGGCAGAGTTCTTCCAGGGTGTCGACGTGATCGTGAACGCCAGCAGCGTGGAAGACATAGACGCGGGTGCCATCGGGCAGCGGCTCTCTAACGGGCTGGCGCGACTCTCCAGCCCTGTGCTGCACCGACTGCGGCTGCGAGAGGATGAGAACGCAGAGCCCGTGGGCACCACCTACCAGAAGACGGATGCAGCTGTGGAAATGAAGCGGATTAACCGAGAGCAGTTCTGGGAGCAGGCCAAGAAGGAAGAAGAGCTGCGGAAGGAGGAGGAGCGGAAGAAGGCCCTGGATGAGAGGCTCAGGTTCGAGCAGGAGCGGATGGAGCAGGAGCGGCAGGAGCAAGAGGAGCGCGAGCGGCGCTACCGGGAGCGGGAGCAGCAGATCGAGGAGCACAGGAGGAAACAGCAGACTTTAGAAGCGGAAGAGGCCAAGAGGCGGTTGAAGGAGCAGTCTATCTTTGGTGACCATCGGGATGAGGAGGAAGAGACCCACATGAAGAAGTCAGAGTCGGAGGTGGAGGAGGCAGCAGCTATTATTGCCCAGCGGCCTGACAACCCAAGGGAGTTCTTCAAGCAGCAGGAAAGAGTCGCATCGGCCTCTGCGGGCAGCTGTGATGTACCCTCGCCCTTCAACCATCGACCAGGCAGCCACCTGGACAGCCACCGGAGGATGGCGCCCACTCCCATCCCCACGCGGAGCCCGTCTGACTCCAGCACCGCCTCCACCCCTGTCGCTGAGCAGATAGAGCGGGCCCTGGATGAGGTCACCTCCTCGCAGCCTCCACCACTGCCACCGCCACCCCCACCAGCCCAAGAGACCCAGGAGCCCAGCCCCATCCTAGACAGTGAGGAGACCAGAGCAGCAGCCCCTCAGGCCTGGGCCGGCCCCATGGAGGAGCCCCCTCAGGCACAGGCGCCTCCCCGGGGGCCAGGCAGCCCTGCAGAGGACTTGATGTTCATGGAGTCTGCAGAGCAGGCTGTCCTGGCTGCTCCCGTGGAGCCTGCCACAGCTGACGCCACGGAGGTCCACGATGCAGCTGACACCATTGAAACTGACACTGCCACTGCTGACACCACTGTTGCCAACAACGTACCCCCCGCCGCCACCAGCCTCATTGACCTATGGCCTGGCAACGGGGAAGGGGCCTCCACACTCCAGGGTGAGCCCAGGGCCCCCACGCCACCCTCGGGTACTGAGGTCACCCTGGCAGAGGTGCCCCTGCTGGATGAGGTGGCTCCGGAGCCACTGCTGCCAGCAGGCGAAGGCTGTGCCACCCTTCTCAACTTTGATGAGCTGCCTGAGCCGCCAGCCACCTTCTGTGACCCAGAGGAAGTGGAAGGGGAGCCCCTGGCTGCCCCCCAGACCCCAACTCTGCCCTCAGCCCTTGAGGAGCTGGAGCAAGAGCAGGAGCCGGAGCCCCACCTGCTAACCAATGGCGAGACCACCCAGAAGGAGGGGACCCAGGCCAGTGAGGGGTACTTCAGTCAATCACAGGAGGAGGAGTTTGCCCAATCGGAAGAGCTCTGTGCCAAGGCTCCGCCTCCTGTGTTCTACAACAAGCCTCCAGAGATCGACATCACATGCTGGGATGCAGACCCAGTTCCAGAAGAGGAGGAGGGCTTCGAGGGTGGTGATTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGVSFSGHRLELLAAYEE
VIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCS
VKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNAS
SVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAA
VEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQERMEQERQEQE
ERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHM
KKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPG
SHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPP
PPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAE
DLMFMESAEQAVLAAPVEPATADATEVHDAADTIETDTATADTTVANNVP
PAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEP
LLPAGEGCATLLNFDELPEPPATFCDPEEVEGEPLAAPQTPTLPSALEEL
EQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPP
VFYNKPPEIDITCWDADPVPEEEEGFEGGD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU38606
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee