cDNA Clone WNK2

• Plasmid: pET-43-WNK2 full wt
• Parent Plasmid: Pet43-mod
• Expressed: Nus-His-WNK2-full wt
• Genbank: NM_006648
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCATGGACGGCGATGGCGGCCGCCGAGACGTCCCCGGCACGCTGATGGAGCCCGGGCGCGGCGCGGGGCCCGCGGGCATGGCGGAGCCTCGGGCGAAGGCGGCGCGGCCGGGGCCCCAGCGCTTTCTGCGGCGCAGCGTGGTAGAGTCGGACCAGGAGGAGCCGCCGGGCTTGGAGGCAGCCGAGGCGCCGGGCCCGCAGCCCCCGCAGCCCCTGCAGCGCCGGGTGCTTCTGCTCTGCAAGACGCGCCGCCTCATCGCGGAGCGCGCTCGCGGACGCCCCGCCGCCCCCGCGCCCGCAGCGCTGGTAGCGCAGCCGGGAGCCCCCGGAGCCCCCGCGGACGCCGGCCCCGAGCCCGTGGGCACGCAGGAGCCCGGCCCGGACCCCATCGCAGCCGCTGTCGAAACCGCGCCTGCCCCCGACGGCGGCCCCAGGGAGGAGGCGGCGGCGACCGTGAGGAAGGAGGATGAGGGGGCGGCCGAGGCGAAGCCTGAGCCCGGGCGCACTCGCCGGGACGAGCCCGAAGAGGAGGAGGACGACGAGGACGACCTCAAGGCCGTGGCCACCTCTCTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGCGGTTCCTTCAAGACGGTCTACAAGGGGCTGGACACGGAGACCTGGGTGGAGGTGGCCTGGTGTGAGCTGCAGGACCGGAAGCTCACCAAGCTGGAGCGGCAGCGGTTCAAGGAAGAGGCTGAGATGCTGAAAGGCCTGCAGCACCCCAACATCGTGCGCTTCTACGACTTCTGGGAGTCCAGCGCCAAGGGCAAGCGGTGCATTGTGCTGGTGACGGAGCTGATGACCTCAGGGACGCTGAAGACATACCTGAAGCGGTTCAAGGTGATGAAGCCCAAGGTTCTCCGCAGCTGGTGCCGGCAGATCCTGAAGGGCCTGCTGTTCCTGCACACAAGGACGCCACCCATCATCCACCGAGACCTGAAATGTGACAATATTTTCATCACCGGACCAACTGGGTCCGTGAAGATTGGCGACTTGGGCCTGGCCACTCTGAAAAGAGCGTCATTTGCCAAAAGTGTGATAGGTACTCCCGAGTTCATGGCGCCCGAGATGTACGAGGAGCACTACGATGAGTCCGTGGACGTCTATGCCTTTGGGATGTGCATGCTGGAGATGGCCACCTCGGAGTACCCCTACTCGGAGTGCCAGAATGCGGCCCAGATCTACCGCAAGGTCACCTGTGGTATCAAGCCGGCCAGCTTTGAGAAAGTGCACGATCCTGAAATCAAGGAGATTATTGGGGAGTGTATCTGCAAAAACAAGGAGGAAAGGTACGAGATCAAAGACCTGCTGAGCCACGCCTTCTTCGCAGAGGACACAGGCGTGAGGGTGGAGCTCGCGGAGGAGGACCACGGCAGGAAGTCCACCATCGCCCTGAGGCTCTGGGTGGAAGACCCCAAGAAACTGAAGGGAAAGCCCAAGGACAATGGAGCCATAGAGTTCACCTTCGACCTGGAGAAGGAGACGCCGGATGAGGTGGCCCAAGAGATGATTGAGTCTGGATTCTTCCACGAGAGTGACGTCAAGATCGTGGCCAAGTCCATCCGTGACCGCGTGGCCTTGATCCAGTGGCGGCGGGAGAGGATCTGGCCCGCGCTGCAGCCCAAGGAGCAGCAGGATGTGGGCAGCCCGGACAAGGCCAGGGGTCCGCCGGTGCCCCTGCAGGTCCAGGTGACCTACCATGCACAGGCTGGGCAGCCCGGGCCACCAGAGCCCGAGGAGCCGGAGGCCGACCAGCACCTCCTGCCACCTACGTTGCCGACCAGCGCCACCTCCCTGGCCTCGGACAGCACCTTCGACAGCGGCCAGGGCTCTACCGTGTACTCAGACTCGCAGAGCAGCCAGCAGAGCGTGATGCTTGGCTCCCTTGCCGACGCAGCGCCGTCCCCGGCCCAGTGTGTGTGCAGCCCCCCTGTGAGCGAGGGGCCCGTCCTGCCGCAGAGCCTGCCCTCGCTGGGGGCCTACCAGCAGCCCACGGCTGCACCTGGCTTGCCGGTGGGCTCTGTCCCGGCCCCCGCCTGCCCTCCGTCCCTCCAGCAGCACTTCCCGGATCCGGCCATGAGCTTCGCCCCCGTGCTGCCGCCGCCCAGCACCCCCATGCCCACGGGCCCAGGCCAGCCAGCACCCCCCGGCCAGCAGCCTCCTCCGCTGGCCCAGCCGACACCCCTGCCGCAGGTCCTGGCCCCACAGCCCGTGGTCCCCCTCCAGCCGGTTCCCCCCCACCTGCCACCGTACCTGGCTCCAGCCTCCCAGGTGGGGGCCCCCGCTCAGCTGAAGCCCCTCCAGATGCCACAGGCGCCCCTGCAGCCGCTTGCTCAAGTCCCTCCGCAGATGCCCCCGATTCCTGTTGTGCCCCCCATCACGCCCCTGGCGGGAATCGACGGCCTCCCTCCGGCCCTCCCAGACCTGCCGACCGCGACTGTGCCTCCCGTGCCACCACCTCAGTATTTCTCTCCAGCCGTGATCTTGCCGAGCCTCGCTGCCCCACTCCCCCCTGCGTCCCCAGCCTTGCCTCTGCAGGCTGTGAAGCTGCCCCACCCCCCTGGGGCGCCCCTGGCCATGCCCTGCCGGACCATTGTGCCAAATGCACCGGCCACTATCCCCCTGCTGGCCGTAGCCCCACCGGGCGTGGCTGCCCTGTCCATTCATTCTGCCGTGGCCCAGCTCCCAGGCCAACCTGTGTACCCAGCGGCCTTCCCACAGATGGCGCCTACTGACGTCCCTCCTTCCCCCCATCACACGGTGCAGAATATGAGGGCCACCCCTCCACAGCCGGCACTGCCTCCACAACCCACACTGCCCCCACAACCCGTGCTGCCCCCGCAACCCACGCTGCCCCCTCAACCTGTGTTGCCCCCGCAACCCACACGGCCCCCTCAACCTGTGCTGCCCCCGCAACCCATGCTGCCCCCACAACCTGTGCTGCCCCCGCAGCCGGCACTGCCTGTGCGCCCTGAGCCCCTCCAGCCCCACCTTCCTGAACAAGCTGCTCCAGCTGCTACACCAGGGAGCCAGATTCTGCTTGGCCACCCAGCTCCCTATGCTGTGGACGTCGCCGCTCAGGTCCCCACCGTGCCTGTGCCACCGGCTGCGGTCCTCTCCCCGCCTCTGCCGGAAGTGCTGCTGCCTGCCGCCCCTGAGCTCCTGCCTCAGTTCCCCAGCTCCCTGGCCACGGTGTCTGCCTCTGTGCAGAGTGTGCCCACCCAGACTGCCACACTTCTGCCACCAGCAAACCCACCGCTGCCTGGCGGGCCCGGGATCGCCAGCCCTTGCCCAACTGTCCAGCTGACGGTGGAACCAGTCCAAGAGGAGCAGGCCTCACAGGACAAGCCGCCCGGCCTCCCGCAGAGCTGTGAGAGCTATGGAGGTTCTGATGTCACTTCTGGAAAAGAGCTGAGTGACAGCTGTGAAGGCGCCTTTGGAGGGGGCAGGCTGGAGGGCAGGGCAGCCCGAAAACACCACCGCAGGTCCACGCGTGCGCGCTCCCGGCAGGAGAGGGCCAGCCGGCCCCGGCTTACCATCTTGAACGTGTGCAACACTGGGGACAAGATGGTGGAGTGCCAGCTGGAGACACACAACCACAAGATGGTGACCTTCAAGTTCGACTTGGACGGGGACGCACCCGATGAAATTGCCACGTATATGGTGGAGCATGACTTTATCCTGCAGGCCGAGCGGGAAACGTTCATCGAGCAGATGAAGGATGTCATGGACAAGGCAGAGGACATGCTCAGCGAGGACACAGACGCCGACCGTGGCTCCGACCCAGGGACCAGCCCGCCACACCTCAGCACCTGCGGCCTGGGCACCGGGGAGGAGAGCCGACAATCCCAAGCCAACGCCCCCGTGTATCAGCAGAACGTCCTGCACACCGGGAAGAGGTGGTTCATCATCTGTCCGGTGGCTGAGCACCCCGCCCCCGAGGCCCCTGAATCTTCGCCCCCACTTCCTCTAAGCTCCCTGCCGCCAGAAGCCAGCCAAGATTCAGCGCCCTATAAAGACCAGCTGTCCTCGAAGGAACAACCCAGCTTTCTAGCCAGTCAGCAGCTCCTGAGCCAGGCGGGCCCCAGCAACCCTCCTGGGGCACCCCCAGCCCCTTTGGCCCCCTCCTCCCCTCCTGTGACTGCTCTGCCCCAAGATGGAGCAGCTCCAGCCACCAGCACCATGCCAGAGCCAGCGTCAGGAACTGCCAGCCAGGCAGGGGGTCCAGGGACACCTCAGGGGCTGACCAGTGAGCTCGAGACGTCTCAGCCACTAGCGGAGACTCACGAGGCCCCGCTTGCTGTGCAGCCCCTCGTGGTGGGCCTAGCACCTTGCACTCCAGCTCCAGAGGCTGCCTCAACCAGGGACGCCAGTGCCCCAAGGGAGCCCCTGCCACCTCCTGCACCTGAGCCCAGCCCCCACAGCGGGACCCCACAGCCCGCCTTGGGTCAGCCTGCTCCCCTGCTTCCTGCCGCAGTGGGGGCCGTCAGCCTGGCCACCTCCCAGCTCCCAAGCCCACCCCTGGGGCCCACCGTCCCCCCACAGCCACCCTCGGCCCTGGAGTCGGATGGGGAAGGGCCGCCCCCCAGGGTGGGCTTTGTGGACAGCACCATCAAGAGCCTGGACGAGAAGCTGCGGACTCTGCTCTACCAGGAGCACGTGCCCACCTCCTCAGCCTCAGCTGGGACCCCTGTGGAGGTGGGCGACAGAGACTTCACCCTGGAGCCCCTGAGAGGGGACCAGCCCCGCTCAGAGGTCTGCGGGGGGGACCTGGCCCTGCCCCCAGTGCCTAAGGAGGCGGTCTCAGGGCGTGTCCAGCTGCCCCAGCCCTTGGTGGAGAAGTCAGAACTGGCCCCCACTCGAGGGGCCGTGATGGAGCAGGGCACGTCCTCGTCAATGACAGCAGAGTCGTCTCCCAGGAGTATGCTAGGCTATGACAGAGATGGAAGGCAGGTGGCCTCAGACTCCCATGTGGTCCCCAGCGTCCCCCAGGATGTACCTGCTTTTGTGAGACCTGCACGTGTGGAGCCCACAGACAGGGATGGTGGAGAAGCTGGAGAAAGCTCGGCAGAGCCCCCGCCGAGTGACATGGGCACAGTGGGGGGCCAGGCTAGCCACCCCCAGACACTCGGCGCTCGAGCTTTGGGGTCCCCTCGGAAACGTCCAGAGCAGCAGGATGTCAGCTCACCAGCCAAGACTGTGGGCCGTTTCTCGGTGGTCAGCACTCAGGACGAGTGGACCCTGGCCTCCCCCCACAGCCTGAGATACTCTGCCCCACCCGACGTCTACCTGGACGAGGCCCCCTCCAGCCCCGACGTGAAGCTGGCAGTGCGGCGGGCGCAGACGGCCTCCTCCATCGAGGTCGGCGTGGGCGAGCCCGTGTCCAGCGACTCTGGGGACGAGGGCCCTCGGGCGAGACCCCCGGTGCAGAAGCAGGCGTCCCTGCCCGTGAGTGGCAGCGTGGCTGGCGACTTCGTGAAGAAGGCCACCGCCTTCCTGCAGAGGCCTTCTCGGGCCGGCTCGCTGGGCCCCGAGACACCCAGCAGGGTGGGCATGAAGGTCCCCACGATCAGCGTGACCTCCTTCCATTCCCAGTCGTCCTACATCAGCAGCGACAATGATTCGGAGCTCGAGGATGCTGACATAAAGAAGGAGCTGCAGAGTCTGCGGGAGAAGCACCTGAAGGAGATCTCGGAGCTGCAGAGCCAGCAGAAGCAGGAGATCGAAGCTCTGTACCGCCGCCTGGGCAAGCCACTGCCCCCCAACGTGGGCTTCTTCCACACGGCACCCCCCACTGGCCGCCGGAGAAAAACCAGCAAGAGCAAGCTGAAGGCAGGCAAGCTGCTAAATCCCCTGGTGCGGCAGCTCAAGGTCGTGGCCTCCAGCACAGGTCACTTGGCTGACTCCAGCAGAGGCCCTCCCGCTAAGGACCCTGCCCAAGCCAGTGTGGGGCTCACTGCAGACAGCACGGGCCTGAGCGGGAAGGCAGTGCAGACCCAGCAGCCCTGCTCCGTCCGGGCCTCCCTGTCTTCGGACATCTGCTCCGGCTTAGCCAGTGATGGAGGCGGAGCGCGTGGCCAAGGCTGGACGGTTTACCACCCAACGTCTGAGAGAGTGACCTATAAGTCTAGTAGCAAACCTCGTGCTCGATTCCTCAGTGGACCCGTATCTGTGTCCATCTGGTCTGCCCTGAAGCGTCTCTGCCTAGGCAAAGAACACAGCAGTAGGTCCTCCACCAGCAGCCTGGCCCCAGGCCCTGAGCCAGGCCCCCAGCCCGCCCTGCACGTCCAGGCGCAGGTGAACAACAGCAACAACAAGAAGGGTACCTTCACGGACGACCTGCACAAGCTGGTGGACGAGTGGACGAGCAAGACGGTGGGGGCCGCGCAGCTGAAGCCCACGCTCAACCAGCTGAAGCAGACCCAGAAGCTGCAAGACATGGAGGCCCAGGCAGGCTGGGCTGCCCCTGGCGAGGCGCGGGCTATGACCGCACCTCGAGCAGGAGTGGGGATGCCACGTCTGCCCCCAGCGCCCGGCCCTCTGTCCACCACGGTCATTCCCGGAGCCGCCCCGACCCTGTCCGTGCCCACACCAGATGGCGCCCTCGGAACCGCCCGGAGAAACCAGGTGTGGTTTGGCCTCCGAGTCCCCCCCACCGCCTGCTGTGGGCACAGCACTCAGCCGCGAGGGGGACAGCGGGTGGGCAGCAAGACTGCTTCCTTTGCGGCTTCAGACCCTGTTCGCTCCTAGGCGGCCGCG

Amino Acid Sequence

MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARLEVLFQGPGSPEFMDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGPVLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTGPGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQMPQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVILPSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIHSAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQPTLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPGSQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASVQSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGGSDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDMLSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAEHPAPEAPESSPPLPLSSLPPEASQDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPATSTMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAASTRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTVPPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDFTLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSDNDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGLSGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFLSGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTDDLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMPRLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQRVGSKTASFAASDPVRS*AAA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU38075
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.