cDNA Clone KCC4

• Plasmid: pEBG-2T-KCC4 wt (potassium/chloride transporter) (Bamh1-Rv-Not)
• Parent Plasmid: pEBG2T
• Expressed: GST-KCC4 wt
• Genbank: NM_006598
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCCACCAACTTCACCGTGGTGCCCGTGGAGGCTCACGCCGACGGCGGCGGGGACGAGACTGCCGAGCGGACGGAGGCTCCGGGCACCCCCGAGGGCCCCGAGCCCGAGCGCCCCAGCCCGGGAGATGGAAATCCAAGAGAAAACAGCCCATTCCTCAACAATGTCGAGGTGGAACAAGAGAGCTTCTTTGAAGGGAAGAACATGGCACTTTTCGAGGAGGAGATGGACAGTAACCCCATGGTGTCCTCGCTGCTCAACAAGCTGGCCAACTACACCAACCTGAGCCAGGGCGTGGTGGAGCACGAGGAGGACGAGGAGAGCCGGCGGCGGGAGGCCAAGGCTCCGCGCATGGGCACCTTCATCGGCGTCTACCTGCCGTGCCTGCAGAACATCCTGGGCGTCATCCTCTTCCTGCGCCTGACGTGGATCGTGGGGGTGGCTGGTGTCCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCACATGTACAATGCTGACCGCCATTTCCATGAGTGCGATCGCTACCAACGGTGTGGTCCCAGCTGGCGGGTCCTACTACATGATATCGCGCTCGCTGGGACCCGAGTTTGGAGGCGCTGTCGGCCTCTGCTTCTACCTGGGCACGACGTTTGCAGGGGCCATGTATATTTTGGGGACCATCGAGATTTTTCTGACGTACATCTCCCCGGGTGCGGCCATCTTCCAGGCGGAGGCTGCAGGTGGCGAGGCGGCCGCCATGCTGCACAACATGCGTGTGTACGGCACGTGCACGCTCGTGCTCATGGCCCTGGTGGTCTTCGTGGGCGTCAAGTATGTCAACAAGCTGGCGCTGGTCTTCCTGGCCTGCGTCGTGCTGTCCATCCTGGCCATCTATGCCGGCGTCATCAAGTCTGCCTTCGACCCCCCGGACATCCCGGTCTGCCTCCTGGGGAACCGCACGCTGTCACGGCGCAGCTTCGATGCCTGCGTCAAGGCCTACGGCATCCACAACAACTCAGCCACCTCCGCGCTCTGGGGCCTCTTCTGCAACGGCTCCCAGCCCAGCGCCGCCTGTGACGAGTACTTCATCCAGAACAACGTCACCGAAATCCAGGGCATCCCGGGCGCGGCCAGTGGTGTCTTCCTGGAGAACCTGTGGAGTACGTACGCGCACGCGGGGGCGTTTGTGGAGAAGAAAGGTGTGCCCTCGGTGCCCGTGGCAGAGGAGAGCCGTGCCAGCACACTGCCCTACGTGCTCACCGACATCGCGGCCTCCTTCACCCTGCTGGTTGGCATCTACTTCCCTTCCGTGACCGGTATCATGGCGGGTTCAAACCGGTCCGGGGACCTCAAGGATGCACAGAAGTCCATCCCCACGGGGACCATCCTGGCCATAGTGACGACGTCTTTCATCTATCTCTCCTGCATTGTGCTGTTTGGGGCCTGCATTGAAGGCGTGGTCTTACGAGATAAGTTCGGGGAGGCCCTGCAGGGGAACCTGGTCATCGGCATGCTGGCCTGGCCCTCCCCCTGGGTCATCGTCATCGGCTCCTTCTTCTCCACCTGCGGTGCCGGCCTGCAGAGCCTCACGGGGGCACCGCGCCTACTGCAGGCCATTGCCCGTGACGGCATCGTCCCCTTCCTGCAGGTGTTTGGCCACGGGAAGGCCAACGGGGAGCCCACGTGGGCGCTGCTGCTGACAGTCCTCATCTGCGAGACTGGCATCCTCATCGCCTCTCTGGACAGCGTGGCCCCGATCCTCTCCATGTTCTTCCTCATGTGCTACCTGTTCGTGAACCTGGCCTGCGCCGTGCAGACCCTGCTACGTACCCCCAACTGGCGTCCACGCTTCAAGTTCTACCACTGGACCCTGTCCTTTCTGGGTATGAGCCTGTGCCTGGCGCTGATGTTCATCTGCTCCTGGTACTACGCGCTGTCCGCCATGCTCATCGCTGGCTGCATCTACAAGTACATCGAGTACCGCGGGGCCGAGAAGGAGTGGGGCGATGGCATCCGTGGCCTATCCCTGAACGCCGCCCGCTACGCCCTGCTGCGCGTGGAGCACGGTCCCCCCCACACCAAGAACTGGAGGCCCCAGGTGCTGGTGATGCTGAACCTGGACGCGGAGCAGGCCGTGAAGCACCCCCGCCTGCTGTCCTTCACGTCGCAGCTGAAGGCCGGCAAGGGCCTGACCATCGTGGGCTCGGTGCTGGAGGGGACGTACCTGGACAAGCACATGGAGGCTCAGCGGGCCGAGGAGAACATACGGTCCCTAATGAGCACAGAGAAGACCAAGGGCTTCTGCCAGCTGGTGGTCTCGTCCAGCCTACGGGATGGCATGTCCCACCTGATCCAGTCGGCCGGCCTGGGCGGCCTGAAGCACAACACGGTGCTCATGGCCTGGCCCGCATCCTGGAAGCAGGAGGACAACCCCTTCTCCTGGAAGAACTTTGTAGACACCGTCCGCGACACCACCGCCGCGCACCAGGCTCTGCTGGTGGCCAAGAACGTCGACTCGTTTCCGCAAAACCAGGAGCGCTTCGGCGGGGGCCACATCGACGTGTGGTGGATCGTGCACGACGGCGGCATGCTCATGCTGCTGCCCTTCCTGCTGCGCCAGCACAAGGTGTGGAGGAAGTGCCGGATGCGTATCTTCACCGTGGCCCAGGTGGACGACAACAGCATCCAGATGAAGAAGGACCTGCAGATGTTCTTGTACCACTTGCGCATCAGCGCCGAGGTGGAGGTGGTGGAGATGGTTGAAAACGACATATCTGCTTTCACCTACGAGAGGACACTAATGATGGAGCAGAGGTCGCAGATGCTGAAGCAGATGCAGCTGTCCAAGAACGAGCAGGAGCGAGAGGCCCAGCTGATCCACGACAGGAACACCGCGTCCCACACCGCGGCGGCAGCCAGGACCCAAGCGCCGCCTACGCCAGACAAGGTGCAGATGACCTGGACCAGGGAGAAGCTGATCGCTGAGAAGTACAGGAGCAGAGACACCAGCCTATCTGGTTTCAAAGACCTCTTCAGCATGAAGCCGGACCAGTCCAACGTCAGGCGGATGCACACGGCTGTGAAGCTCAATGGCGTCGTCCTCAACAAGTCCCAGGATGCGCAGCTGGTCCTGCTCAACATGCCAGGTCCTCCCAAAAACCGGCAGGGAGACGAGAACTACATGGAGTTTCTTGAAGTCCTGACCGAGGGGCTGAACAGAGTCCTCCTGGTCAGGGGTGGCGGCCGGGAGGTGATCACCATCTACTCCTAAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSMPTNFTVVPVEAHADGGGDETAERTEAPGTPEGPEPERPSPGDGNPRENSPFLNNVEVEQESFFEGKNMALFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEESRRREAKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGVAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEIFLTYISPGAAIFQAEAAGGEAAAMLHNMRVYGTCTLVLMALVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKSAFDPPDIPVCLLGNRTLSRRSFDACVKAYGIHNNSATSALWGLFCNGSQPSAACDEYFIQNNVTEIQGIPGAASGVFLENLWSTYAHAGAFVEKKGVPSVPVAEESRASTLPYVLTDIAASFTLLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTVLICETGILIASLDSVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALMFICSWYYALSAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNLDAEQAVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHMEAQRAEENIRSLMSTEKTKGFCQLVVSSSLRDGMSHLIQSAGLGGLKHNTVLMAWPASWKQEDNPFSWKNFVDTVRDTTAAHQALLVAKNVDSFPQNQERFGGGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFTYERTLMMEQRSQMLKQMQLSKNEQEREAQLIHDRNTASHTAAAARTQAPPTPDKVQMTWTREKLIAEKYRSRDTSLSGFKDLFSMKPDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKNRQGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS*GS

• Vector Type: Mammalian
• DU Number: DU30466
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee