cDNA Clone IRLB

• Plasmid: pGEX6P-1-IRLB
• Parent Plasmid: pGEX6P1
• Expressed: GST-IRLB
• Genbank: NP_005839.2 GI:125628677
• Tag: GST
• Species: Human

Sequence of Insert

gaattcatgattgaagacaaggggcctcgtgttgctgactactttgttgt
agcaggattaactgatgtttcaaagcctctagaagaagaaattcacttca
atgatgcttgtcataaagtagctaaaccaaaagaacctattacagatgtt
tcagttattatcaaatctcttggggaggaagtcccacaggattatatctg
tattgatgttaccccaactggattgtcagctgatcttaataatggaagtc
ttgtggggccacagatttacctttgctatagaagaggaagagataagcct
ccacttacagatctgggggttttatatgactggaaagaaagattgaaaca
gggttgtgaaattattcagagtactccctatgggcgccccgcaaatatta
gtgggagtacctcatcacaaagaatttatatcacttaccgaagagcctct
gaaaacatgactcagaatacgttggctgtcactgacatatgtattattat
acccagtaaaggagaaagcccaccacacacgttctgcaaagtcgacaaga
atctcaataacagtatgtggggatcagcagtatacttgtgttataaaaaa
tcggtggcaaagacgaacactgtatcttacaaagctggcctaatttgtag
atatcctcaagaagattatgagtcattctcactaccggaatctgttcctc
tattttgtttaccaatgggtgcaaccattgaatgttggccatcaaatagc
aaataccctctgcctgtattttctacttttgttttaactggagcctcagc
tgaaaaggtttatggtgctgctattcagttttatgaaccatactctgagg
agaatcccacagaaaagcagagacttcttttgggtttaacatcagcagat
gggaagtctgatagttccaaaacaattcatactaacaaatgcatctgtct
tctttctcactggcctttttttgatgcattcaggaagtttctgacttttc
tgtatcgttattccatctctgggcctcatgtccttccaattgagaagcat
atttctcattttatgcataaagttccttttccatctcctcagagaccacg
gattttagttcagttatcaccacatgataatctgattctcagtcagcctg
tgtcttcacctcttccactaagtggtggcaagttttcaacactactgcag
aatttaggccctgaaaatgctgtgacactactggtgtttgcagtaacaga
acataaaattcttatccattccctacggccatccgtgcttactagtgtga
cagaagcattagtgtctatgattttccctttccactggccatgcccgtat
gttcctctctgcccactggctttagcagatgtcttgagtgcaccatgtcc
attcatagtagggattgattcaagatactttgatctctatgatcctccac
cagatgtcagttgtgttgatgtagataccaacactatttctcaaattggt
gacaagaaaaatgtagcctggaagatacttccaaagaagccatgtaaaaa
tctgatgaacacactgaataatttgcatcagcaattggcaaaattgcagc
agagaccgagagatgatggactcatggatttagcaataaatgactatgat
tttaattctggaaagaggttgcacatgatagatttggaaatccaagaggc
atttttgtttttcatggcctctattttaaaaggttacagatcatacttaa
ggccaataacacaagccccatctgagacagccacagatgcagcctctctc
tttgccctacaagctttcttaagaagccgggaccggtcacatcaaaaatt
ctataacatgatgaccaaaacacaaatgtttattcgcttcattgaagaat
gttcttttgttagtgacaaagatgcaagcctggcattttttgatgattgt
gtagataaagtggatatggacaagagcggtgaagtacgacttatagaact
ggatgaatctttcaaaagtgaacacactgtttttgtaacaccacctgaga
tcccccacctacccaatggtgaagaaccccctttacagtacagctataat
ggatttccagttcttaggaacaatttatttgaaagacctgaaggatttct
acaagcaaagaagaacaaattgccttcaaaatcaagtagtcctaatagcc
ctttgcccatgttcagaagaacaaaacaggaaattaaatcagcccataaa
attgcaaagaggtactcttccatccctcagatgtggtctaggtgtctact
gcgccactgttatggactgtggtttatttgtctcccagcttatgtgaaag
tctgtcattcaaaagtcagggctctgaaaacagcatatgatgtgcttaaa
aaaatgcagtcaaagaagatggatccacctgatgaggtatgctaccgcat
tcttatgcaactctgtggacaatatgatcagccagtgcttgcagttcgag
tgctttttgaaatgcagaaagctggtattgaccccaatgccattacttat
ggttattataataaggctgttttggaaagtacctggccttcaagaagtcg
tagtggctattttctttggacaaaagtaagaaatgttgttttaggagtaa
cacagttcaaaagagctttaaagaagcatgcacacttatcacaaacaact
ctctcaggtggccagtctgatcttggatataattcattatctaaagatga
agttagaagaggggatacatctactgaagacattcaagaagaaaaagata
aaaaagggagtgattgtagttccttgtcagagagtgagagtacaaaagga
agtgctgattgccttcctaagctcagttatcaaaattcttccagtatcgt
tcgcctcactggtacaagtaacaacagtgctggtaaaatatcaggagaaa
gcatggaaagcacacctgagctgctcttaatatcatctcttgaagataca
aatgaaacacgaaacattcaaagtagatgcttcaggaaaagacataaaag
tgacaatgaaactaatttgcagcagcaagtggtctggggaaatagaaacc
gtaatcttagtggaggggtactgatgggatttatgctcaatagaattaac
caggaagcaacccctggagatatagttgaaaaattgggagctgatgcaaa
aattctttcaaatgttatctcaaaaagcacgagaccaaatactcttgata
ttgggaagccacctttaagatcaaaaagagacagtctagaaaaggaatct
agtgatgatgatacaccttttgatggttctaactatttggcagataaagt
ggattctcctgttatttttgacttagaagacttagacagtgaaacagatg
tatcaaaagcaggatgtgttgctacacaaaatcccaagaggattcaacgt
atgaacagcagcttttcagtaaaaccttttgaaaaaactgatgtcgcaac
aggatttgatcccctctctcttttggttgctgagactgaacagcagcaaa
aagaagaagaggaggaggatgaagatgatagcaaaagcatttcaacacca
tctgctaggcgtgatttagctgaagaaattgtgatgtatatgaataacat
gagcagtcctttgacaagtcgtacaccaagcattgatttacaacgggcat
gtgatgataaattaaataagaaaagtccaccattggtcaaggcatgcaga
agatctagtctgcctcctaattctcctaagccagtgagacttaccaaatc
taaaagttacacaaaaagtgaggagaaaccaagggatagactttggtctt
ctccagccttctcacctacttgcccatttagggaagaatctcaggataca
ttaacccactcatcaccttcatttaacttagatacactactagtacctaa
actagatgttctaagaaacagtatgttcactgctggaaaaggagttgcag
agaaagcaagcaaatggtattcaagattcaccatgtacaccacatcatct
aaggatcaaagttctgatcgtaccagtctttcttcagtgggagcccagga
ttctgaatctacctctttaacagatgaagatgtctgccatgagttggaag
gacctatctcctctcaagagaccagtgctacttcagggactaagagaatt
gatctcagccgaataagcctggaaagttctgcatccttggaaggatctct
gtcgaagtttgccttacctgggaaatcagaagtgacatcttccttcaacg
cgagtaatacaaatatcttccagaactatgcaatggaggttctcatctca
agttgctctcggtgtagaacttgtgattgtcttgtccatgatgaggaaat
catggctggctggacagcagatgattcaaatctcaatactacatgcccat
tctgtggcaatatcttcttaccctttctgaatatagaaataagagattta
agacgacctggaagatactttctaaagtcaagcccatcaacagaaaatat
gcactttccatcctccatttcaagtcagacgaggcagtcttgcatttcaa
catcagcctctggtcttgacacatctgctctctctgttcaagggaatttt
gatctaaatagcaaatctaaactgcaggaaaatttttgcacccgaagtat
tcagatccctgctaatagatcaaaaacagctatgtctaaatgtccaatat
ttccaatggccaggagtattagtacttctggccccttggataaggaagat
actggaagacagaagctcatttctacaggcagcctgccagctactttaca
aggagctacagattctttaggtttagaatggcaccttccaagtcccgatc
ctgtcactgttccgtatcttagtcctttagtggtatggaaagaacttgaa
agcttattggaaaatgaaggtgatcatgcaataacagtagcagactttgt
ggaccatcatccaattgttttttggaacctggtatggtatttcagacgtc
ttgacttgcccagtaacttacctggattgattctttcttctgagcactgt
aacaagtattcaaagattccccgccactgtatgtctgaagatagcaaata
tgttttaatacaaatgttatgggacaatatgaagttacatcaggatccgg
gacagcccttgtacattctctggaatgcacacacccaaaaatatccaatg
gtccatttattgcaaaaaagtgataactcatttaaccaggaactgttgaa
aagtatggtaaaaagcattaaaatgaatgatgtctatggaccaatgagtc
agattttagagacactgaataagtgtccacattttaaaagacagaggagt
ttatacagagaaatactatttctctcacttgtggcactgggaagagaaaa
cattgatatagatgcttttgataaagagtacaagatggcatacgatcgtc
tcacacctagtcaagtcaagagtacacacaactgtgatagaccaccaagt
acaggggtgatggaatgtcgaaaaacctttggagaaccttatctttaagc
ggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEI
IQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKG
ESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQE
DYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVY
GAAIQFYEPYSEENPTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHW
PFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQ
LSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKIL
IHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVG
IDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNT
LNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFF
MASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMM
TKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESF
KSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKK
NKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCY
GLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQL
CGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYF
LWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRG
DTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTG
TSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNET
NLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSN
VISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPV
IFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDP
LSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPL
TSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYT
KSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVL
RNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSEST
SLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFA
LPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGW
TADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPS
SISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPA
NRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATD
SLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHP
IVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQ
MLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVK
SIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDID
AFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16176
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 236420 Daltons; ; All IRLB plasmids MUST be grown at 30 degrees C or less to prevent recombination.