cDNA Clone IRLB

• Plasmid: pCMV5-HA IRLB
• Parent Plasmid: pCMV5 HA1
• Expressed: HA IRLB
• Genbank: NP_005839.2 GI:125628677*
• Tag: HA
• Species: Human

Sequence of Insert

gaattcatgattgaagacaaggggcctcgtgttgctgactactttgttgt
agcaggattaactgatgtttcaaagcctctagaagaagaaattcacttca
atgatgcttgtcataaagtagctaaaccaaaagaacctattacagatgtt
tcagttattatcaaatctcttggggaggaagtcccacaggattatatctg
tattgatgttaccccaactggattgtcagctgatcttaataatggaagtc
ttgtggggccacagatttacctttgctatagaagaggaagagataagcct
ccacttacagatctgggggttttatatgactggaaagaaagattgaaaca
gggttgtgaaattattcagagtactccctatgggcgccccgcaaatatta
gtgggagtacctcatcacaaagaatttatatcacttaccgaagagcctct
gaaaacatgactcagaatacgttggctgtcactgacatatgtattattat
acccagtaaaggagaaagcccaccacacacgttctgcaaagtcgacaaga
atctcaataacagtatgtggggatcagcagtatacttgtgttataaaaaa
tcggtggcaaagacgaacactgtatcttacaaagctggcctaatttgtag
atatcctcaagaagattatgagtcattctcactaccggaatctgttcctc
tattttgtttaccaatgggtgcaaccattgaatgttggccatcaaatagc
aaataccctctgcctgtattttctacttttgttttaactggagcctcagc
tgaaaaggtttatggtgctgctattcagttttatgaaccatactctgagg
agaatcccacagaaaagcagagacttcttttgggtttaacatcagcagat
gggaagtctgatagttccaaaacaattcatactaacaaatgcatctgtct
tctttctcactggcctttttttgatgcattcaggaagtttctgacttttc
tgtatcgttattccatctctgggcctcatgtccttccaattgagaagcat
atttctcattttatgcataaagttccttttccatctcctcagagaccacg
gattttagttcagttatcaccacatgataatctgattctcagtcagcctg
tgtcttcacctcttccactaagtggtggcaagttttcaacactactgcag
aatttaggccctgaaaatgctgtgacactactggtgtttgcagtaacaga
acataaaattcttatccattccctacggccatccgtgcttactagtgtga
cagaagcattagtgtctatgattttccctttccactggccatgcccgtat
gttcctctctgcccactggctttagcagatgtcttgagtgcaccatgtcc
attcatagtagggattgattcaagatactttgatctctatgatcctccac
cagatgtcagttgtgttgatgtagataccaacactatttctcaaattggt
gacaagaaaaatgtagcctggaagatacttccaaagaagccatgtaaaaa
tctgatgaacacactgaataatttgcatcagcaattggcaaaattgcagc
agagaccgagagatgatggactcatggatttagcaataaatgactatgat
tttaattctggaaagaggttgcacatgatagatttggaaatccaagaggc
atttttgtttttcatggcctctattttaaaaggttacagatcatacttaa
ggccaataacacaagccccatctgagacagccacagatgcagcctctctc
tttgccctacaagctttcttaagaagccgggaccggtcacatcaaaaatt
ctataacatgatgaccaaaacacaaatgtttattcgcttcattgaagaat
gttcttttgttagtgacaaagatgcaagcctggcattttttgatgattgt
gtagataaagtggatatggacaagagcggtgaagtacgacttatagaact
ggatgaatctttcaaaagtgaacacactgtttttgtaacaccacctgaga
tcccccacctacccaatggtgaagaaccccctttacagtacagctataat
ggatttccagttcttaggaacaatttatttgaaagacctgaaggatttct
acaagcaaagaagaacaaattgccttcaaaatcaagtagtcctaatagcc
ctttgcccatgttcagaagaacaaaacaggaaattaaatcagcccataaa
attgcaaagaggtactcttccatccctcagatgtggtctaggtgtctact
gcgccactgttatggactgtggtttatttgtctcccagcttatgtgaaag
tctgtcattcaaaagtcagggctctgaaaacagcatatgatgtgcttaaa
aaaatgcagtcaaagaagatggatccacctgatgaggtatgctaccgcat
tcttatgcaactctgtggacaatatgatcagccagtgcttgcagttcgag
tgctttttgaaatgcagaaagctggtattgaccccaatgccattacttat
ggttattataataaggctgttttggaaagtacctggccttcaagaagtcg
tagtggctattttctttggacaaaagtaagaaatgttgttttaggagtaa
cacagttcaaaagagctttaaagaagcatgcacacttatcacaaacaact
ctctcaggtggccagtctgatcttggatataattcattatctaaagatga
agttagaagaggggatacatctactgaagacattcaagaagaaaaagata
aaaaagggagtgattgtagttccttgtcagagagtgagagtacaaaagga
agtgctgattgccttcctaagctcagttatcaaaattcttccagtatcgt
tcgcctcactggtacaagtaacaacagtgctggtaaaatatcaggagaaa
gcatggaaagcacacctgagctgctcttaatatcatctcttgaagataca
aatgaaacacgaaacattcaaagtagatgcttcaggaaaagacataaaag
tgacaatgaaactaatttgcagcagcaagtggtctggggaaatagaaacc
gtaatcttagtggaggggtactgatgggatttatgctcaatagaattaac
caggaagcaacccctggagatatagttgaaaaattgggagctgatgcaaa
aattctttcaaatgttatctcaaaaagcacgagaccaaatactcttgata
ttgggaagccacctttaagatcaaaaagagacagtctagaaaaggaatct
agtgatgatgatacaccttttgatggttctaactatttggcagataaagt
ggattctcctgttatttttgacttagaagacttagacagtgaaacagatg
tatcaaaagcaggatgtgttgctacacaaaatcccaagaggattcaacgt
atgaacagcagcttttcagtaaaaccttttgaaaaaactgatgtcgcaac
aggatttgatcccctctctcttttggttgctgagactgaacagcagcaaa
aagaagaagaggaggaggatgaagatgatagcaaaagcatttcaacacca
tctgctaggcgtgatttagctgaagaaattgtgatgtatatgaataacat
gagcagtcctttgacaagtcgtacaccaagcattgatttacaacgggcat
gtgatgataaattaaataagaaaagtccaccattggtcaaggcatgcaga
agatctagtctgcctcctaattctcctaagccagtgagacttaccaaatc
taaaagttacacaaaaagtgaggagaaaccaagggatagactttggtctt
ctccagccttctcacctacttgcccatttagggaagaatctcaggataca
ttaacccactcatcaccttcatttaacttagatacactactagtacctaa
actagatgttctaagaaacagtatgttcactgctggaaaaggagttgcag
agaaagcaagcaaatggtattcaagattcaccatgtacaccacatcatct
aaggatcaaagttctgatcgtaccagtctttcttcagtgggagcccagga
ttctgaatctacctctttaacagatgaagatgtctgccatgagttggaag
gacctatctcctctcaagagaccagtgctacttcagggactaagagaatt
gatctcagccgaataagcctggaaagttctgcatccttggaaggatctct
gtcgaagtttgccttacctgggaaatcagaagtgacatcttccttcaacg
cgagtaatacaaatatcttccagaactatgcaatggaggttctcatctca
agttgctctcggtgtagaacttgtgattgtcttgtccatgatgaggaaat
catggctggctggacagcagatgattcaaatctcaatactacatgcccat
tctgtggcaatatcttcttaccctttctgaatatagaaataagagattta
agacgacctggaagatactttctaaagtcaagcccatcaacagaaaatat
gcactttccatcctccatttcaagtcagacgaggcagtcttgcatttcaa
catcagcctctggtcttgacacatctgctctctctgttcaagggaatttt
gatctaaatagcaaatctaaactgcaggaaaatttttgcacccgaagtat
tcagatccctgctaatagatcaaaaacagctatgtctaaatgtccaatat
ttccaatggccaggagtattagtacttctggccccttggataaggaagat
actggaagacagaagctcatttctacaggcagcctgccagctactttaca
aggagctacagattctttaggtttagaatggcaccttccaagtcccgatc
ctgtcactgttccgtatcttagtcctttagtggtatggaaagaacttgaa
agcttattggaaaatgaaggtgatcatgcaataacagtagcagactttgt
ggaccatcatccaattgttttttggaacctggtatggtatttcagacgtc
ttgacttgcccagtaacttacctggattgattctttcttctgagcactgt
aacaagtattcaaagattccccgccactgtatgtctgaagatagcaaata
tgttttaatacaaatgttatgggacaatatgaagttacatcaggatccgg
gacagcccttgtacattctctggaatgcacacacccaaaaatatccaatg
gtccatttattgcaaaaaagtgataactcatttaaccaggaactgttgaa
aagtatggtaaaaagcattaaaatgaatgatgtctatggaccaatgagtc
agattttagagacactgaataagtgtccacattttaaaagacagaggagt
ttatacagagaaatactatttctctcacttgtggcactgggaagagaaaa
cattgatatagatgcttttgataaagagtacaagatggcatacgatcgtc
tcacacctagtcaagtcaagagtacacacaactgtgatagaccaccaagt
acaggggtgatggaatgtcgaaaaacctttggagaaccttatctttaagc
ggccgc

Amino Acid Sequence

MYPYDVPDYAGSPEFMIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDAC
HKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGP
QIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGST
SSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNN
SMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCL
PMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENPT
EKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRY
SISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSP
LPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEAL
VSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVS
CVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPR
DDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPIT
QAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFV
SDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHL
PNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPM
FRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHS
KVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFE
MQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFK
RALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGS
DCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMES
TPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLS
GGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKP
PLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKA
GCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEE
EEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDK
LNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAF
SPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKAS
KWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPIS
SQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNT
NIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGN
IFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSAS
GLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMA
RSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTV
PYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLP
SNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPL
YILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILE
TLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPS
QVKSTHNCDRPPSTGVMECRKTFGEPYL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16136
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This sequence has since been updated in NCBI to NP_005839.3 GI:221307566 in which residue 284 is Leu, not Pro.; ; Estimated MW of expressed protein : 210956 Daltons