cDNA Clone HAP1A (rat)

• Plasmid: pETBlue2.6His rat HAP1A
• Parent Plasmid: pETBlue2 6His
• Genbank: NM_024133*
• Tag: 6His
• Species: Rat

Sequence of Insert

GAATTCATGCGCCCGAAGGACCAGGTGCAGAGCAGTGCCGGGGACGGGAC
GGGGTCGGGGGACCCAGCAACAGGCACCCCCACGACCCAGCCTGCAGCGG
ATCCCGCTCCGGAGCCCTCGGCAGAGCCCAAACCTGCTCCGGCGCAGGGA
ACCGGGTCCGGACAGAAATCAGGATCCCGAACCAAGACAGGAGGAAGCTT
TTGTAGGTCCAGGATCCGTGGCGACTCGGACGCACCATGGACCCGCTACA
TATTCCAGGGGCCTTACGGTCCCCGGGCTACTGGCCTGGGCACTGGAAGG
GCTGAGGGAATCTGGAAGACGCCAGCCGCGTACATCGGCCGAAGGCCCGG
CGTGTCCGGCCCTGAGCGCGCGGCGTTTATTCGAGAGCTGCAGGAAGCGC
TGTGTCCTAATCCACTGCCCAGGAAGAAGATCACCGAAGATGATATCAAA
GTGATGTTGTATTTGCTGGAAGAGAAAGAACGGGACCTGAACACAGCCGC
TCGCATCGGCCAGTCCCTGGTGAAACAGAATAGTGTTCTGATGGAGGAGA
ATAACAAGCTGGAAACCATGCTGGGCTCAGCCAGAGAGGAGATTTTACAT
CTCCGGAAGCAGGTGAACCTGCGAGATGATCTCCTTCAGCTCTACTCGGA
CTCCGATGACGATGAGGAGGATGAAGAGGATGAGGAAGAGGAAGAGGGAG
AAGAGGAGGAACGAGAAGGACAGAGGGACCAAGATCAGCAGCACGACCAT
CCCTATGGTGCCCCCAAGCCGCCCCCTAAGGCTGAGACGCTGCACCACTG
CCCACAGCTGGAAGCCCTGAAGCAGAAGCTGAAACTGCTGGAAGAAGAGA
ACGACCATCTTCGAGAGGAGGCCTCCCACCTTGACAACCTGGAAGACAAA
GAACAGATGCTCATTCTGGAGTGTGTGGAACAGTTTTCTGAAGCCAGCCA
GCAGATGGCAGAGCTATCCGAGGTGTTGGTGCTGAGGCTGGAAGGCTATG
AGAGGCAGCAGAAGGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTA
CAACAGCGTTGTCAGTCTTATGGGGCCCAGACGGAGAAACTGCAGCAGCA
GCTGGCCTCAGAGAAGGGAGTCCACCCAGAGAGCCTGCGAGCTGGCTCCC
ACATGCAGGATTATGGAAGCAGGCCTCGTGAACGCCAGGAGGATGGGAAG
AGCCATCGTCAGCGTTCCTCAATGCCTGCAGGTTCTGTCACCCACTATGG
ATACAGTGTGCCTCTGGATGCACTTCCAAGTTTCCCAGAGACACTGGCTG
AGGAGCTCCGGACATCCCTGAGGAAGTTCATCACTGACCCTGCGTATTTC
ATGGAGAGATGTGACACTCGCTGCAGAGAGGAACGAAAGAAGGAGCAGGG
GGCAATGCCACCCCCACCGGCGCAAGATCTCAAGCCGCCTGAAGATTTCG
AGGCTCCAGAGGAGCTGGTTCCTGAGGAGGAGCTGGGGGCCATCGAAGAG
GTGGGGACAGCTGAGGATGGGCCGGCAGAAGAGACAGAGCAGACATCTGA
GGAGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGGCCACAA
GGATGAATGTGGTGGTCTCTGCCCTGGAGGCCAGCGGCCTGGGCCCTTCA
CACCTGGACATGAAGTATGTCCTCCAGCAACTGTCCAACTGGCAGGACGC
CCATTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCGAAAGGTGAGTGTT
CCCGCAGAGGACACCCTCCTGCCAGTGGGACAAGCTACCGATCATCAACC
CTATGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSPEFMRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPE
PSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRIRGDSDAPWTRYIFQGP
YGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNP
LPRKKITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLE
TMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDEEDEEDEEEEEGEEEER
EGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEEENDHLR
EEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQK
EITQLQAEITKLQQRCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDY
GSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRT
SLRKFITDPAYFMERCDTRCREERKKEQGAMPPPPAQDLKPPEDFEAPEE
LVPEEELGAIEEVGTAEDGPAEETEQTSEETEAWEEVEPEVDEATRMNVV
VSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKGECSRRGH
PPASGTSYRSSTL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16131
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct.; Sequence differences compared with NM_024133 CDS: g1488c and g1293t (both silent); T466A - present in other Rat HAP1A published seqs.; V472A and A513T - not in published Rat seqs but ARE in published mouse seqs.