cDNA Clone HAP1A (rat)

• Plasmid: pEBG6P-HAP1A (rat)
• Parent Plasmid: pEBG6P
• Genbank: NM_024133*
• Species: Rat

Sequence of Insert

ACTAGTATGCGCCCGAAGGACCAGGTGCAGAGCAGTGCCGGGGACGGGAC
GGGGTCGGGGGACCCAGCAACAGGCACCCCCACGACCCAGCCTGCAGCGG
ATCCCGCTCCGGAGCCCTCGGCAGAGCCCAAACCTGCTCCGGCGCAGGGA
ACCGGGTCCGGACAGAAATCAGGATCCCGAACCAAGACAGGAGGAAGCTT
TTGTAGGTCCAGGATCCGTGGCGACTCGGACGCACCATGGACCCGCTACA
TATTCCAGGGGCCTTACGGTCCCCGGGCTACTGGCCTGGGCACTGGAAGG
GCTGAGGGAATCTGGAAGACGCCAGCCGCGTACATCGGCCGAAGGCCCGG
CGTGTCCGGCCCTGAGCGCGCGGCGTTTATTCGAGAGCTGCAGGAAGCGC
TGTGTCCTAATCCACTGCCCAGGAAGAAGATCACCGAAGATGATATCAAA
GTGATGTTGTATTTGCTGGAAGAGAAAGAACGGGACCTGAACACAGCCGC
TCGCATCGGCCAGTCCCTGGTGAAACAGAATAGTGTTCTGATGGAGGAGA
ATAACAAGCTGGAAACCATGCTGGGCTCAGCCAGAGAGGAGATTTTACAT
CTCCGGAAGCAGGTGAACCTGCGAGATGATCTCCTTCAGCTCTACTCGGA
CTCCGATGACGATGAGGAGGATGAAGAGGATGAGGAAGAGGAAGAGGGAG
AAGAGGAGGAACGAGAAGGACAGAGGGACCAAGATCAGCAGCACGACCAT
CCCTATGGTGCCCCCAAGCCGCCCCCTAAGGCTGAGACGCTGCACCACTG
CCCACAGCTGGAAGCCCTGAAGCAGAAGCTGAAACTGCTGGAAGAAGAGA
ACGACCATCTTCGAGAGGAGGCCTCCCACCTTGACAACCTGGAAGACAAA
GAACAGATGCTCATTCTGGAGTGTGTGGAACAGTTTTCTGAAGCCAGCCA
GCAGATGGCAGAGCTATCCGAGGTGTTGGTGCTGAGGCTGGAAGGCTATG
AGAGGCAGCAGAAGGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTA
CAACAGCGTTGTCAGTCTTATGGGGCCCAGACGGAGAAACTGCAGCAGCA
GCTGGCCTCAGAGAAGGGAGTCCACCCAGAGAGCCTGCGAGCTGGCTCCC
ACATGCAGGATTATGGAAGCAGGCCTCGTGAACGCCAGGAGGATGGGAAG
AGCCATCGTCAGCGTTCCTCAATGCCTGCAGGTTCTGTCACCCACTATGG
ATACAGTGTGCCTCTGGATGCACTTCCAAGTTTCCCAGAGACACTGGCTG
AGGAGCTCCGGACATCCCTGAGGAAGTTCATCACTGACCCTGCGTATTTC
ATGGAGAGATGTGACACTCGCTGCAGAGAGGAACGAAAGAAGGAGCAGGG
GGCAATGCCACCCCCACCGGCGCAAGATCTCAAGCCGCCTGAAGATTTCG
AGGCTCCAGAGGAGCTGGTTCCTGAGGAGGAGCTGGGGGCCATCGAAGAG
GTGGGGACAGCTGAGGATGGGCCGGCAGAAGAGACAGAGCAGACATCTGA
GGAGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGGCCACAA
GGATGAATGTGGTGGTCTCTGCCCTGGAGGCCAGCGGCCTGGGCCCTTCA
CACCTGGACATGAAGTATGTCCTCCAGCAACTGTCCAACTGGCAGGACGC
CCATTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCGAAAGGTGAGTGTT
CCCGCAGAGGACACCCTCCTGCCAGTGGGACAAGCTACCGATCATCAACC
CTATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMRPKDQVQSSA
GDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKT
GGSFCRSRIRGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIG
RRPGVSGPERAAFIRELQEALCPNPLPRKKITEDDIKVMLYLLEEKERDL
NTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQ
LYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAET
LHHCPQLEALKQKLKLLEEENDHLREEASHLDNLEDKEQMLILECVEQFS
EASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEK
LQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSV
THYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERK
KEQGAMPPPPAQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGPAEETE
QTSEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLSN
WQDAHSKRQQKQKVVPKGECSRRGHPPASGTSYRSSTL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16009
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_024133 CDS: g1488c and g1293t (both silent); T466A - present in other Rat HAP1A published seqs.; V472A and A513T - not in published Rat seqs but ARE in published mouse seqs.; ; Mammalian expression plasmid to express Rat HAP1A with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 94578 Daltons