cDNA Clone Nudel (NDEL1-FMGQ)

• Plasmid: pGEX6P-2-Nudel isoform A
• Parent Plasmid: pGEX6P1
• Expressed: GST-Nudel isoform A
• Genbank: NM_001025579.1 (Homo sapiens nudE nuclear distribution gene E homolog (A.nidulans)-like 1 (NDEL1), transcript variant 1)
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCatggatggtgaagatataccagatttttcaagtttaaaggaggaaactgcttattggaaggaactttccttgaagtataagcaaagcttccaggaagctcgggatgagctagttgaattccaggaaggaagcagagaattagaagcagagttggaggcacaattagtacaggctgaacaaagaaatagagacttgcaggctgataaccaaagactgaaatatgaagtggaggcattaaaggagaagctagagcatcaatatgcacagagctataagcaggtctcagtgttagaagatgat
ttaagtcagactcgggccattaaggagcagttgcataagtatgtgagaga
gctggagcaggccaacgacgacctggagcgagccaaaagggcaacaatag
tttcactggaagactttgaacaaaggctaaaccaggccattgaacgaaat
gcatttttagaaagtgaacttgatgaaaaggaatctttgttggtctctgt
acagaggttaaaggatgaagcaagagatttaaggcaagaactagcagttc
gggaaagacaacaggaagtaactagaaagtcggctcctagctctccaact
ctagactgtgaaaagatggactccgccgtccaagcatcactttctttgcc
agctacccctgttggcaaaggaacggagaacacttttccttcaccgaaag
ctataccaaatggttttggtaccagtccactaactccctctgctaggata
tcagcactaaacatcgtgggggatctcttacggaaagtaggggctttaga
atccaaattagcagcttgcaggaattttgcaaaggaccaagcatcacgaa
aatcctatatttcagggaatgttaactgtggggtgctgaatggcaatggc
acaaagttctctcgatcagggcatacatctttcttcgacaaagggcaaga
aaaagtcatatttcccacgttgttcatggggcagtaaGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDGEDIPDFSSLKEETAYW
KELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADN
QRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELE
QANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQR
LKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPAT
PVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESK
LAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGQEKV
IFPTLFMGQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11924
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 63861 Daltons