cDNA Clone LIS1

• Plasmid: pETBlue2.6His LIS1
• Parent Plasmid: pETBlue2 6His
• Expressed: 6His LIS1
• Genbank: NM_000430.2
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGGTGCTGTCCCAGAGACAACGAGATGAACTAAATCGAGCTAT
AGCAGATTATCTTCGTTCAAATGGCTATGAAGAGGCATATTCAGTTTTTA
AAAAGGAAGCTGAATTAGATGTGAATGAAGAATTAGATAAAAAGTATGCT
GGTCTTTTGGAAAAAAAATGGACATCTGTTATTAGATTACAAAAGAAGGT
TATGGAATTAGAATCAAAGCTAAATGAAGCAAAAGAAGAATTTACGTCAG
GTGGACCTCTTGGTCAGAAACGAGACCCAAAAGAATGGATTCCCCGTCCG
CCAGAAAAATATGCATTGAGTGGTCACAGGAGTCCAGTCACTCGAGTCAT
TTTCCATCCTGTGTTCAGTGTTATGGTCTCTGCTTCAGAGGATGCTACAA
TTAAGGTGTGGGATTATGAGACTGGAGATTTTGAACGAACTCTTAAAGGA
CATACAGACTCTGTACAGGACATTTCATTCGACCACAGCGGCAAGCTTCT
GGCTTCCTGTTCTGCAGATATGACCATTAAACTATGGGATTTTCAGGGCT
TTGAATGCATCAGAACCATGCACGGCCATGACCACAATGTTTCTTCAGTA
GCCATCATGCCCAATGGAGATCATATAGTGTCTGCCTCAAGGGATAAAAC
TATAAAAATGTGGGAAGTGCAAACTGGCTACTGTGTGAAGACATTCACAG
GACACAGAGAATGGGTACGTATGGTACGGCCAAATCAAGATGGCACTCTG
ATAGCCAGCTGTTCCAATGACCAGACTGTGCGTGTATGGGTCGTAGCAAC
AAAGGAATGCAAGGCTGAGCTCCGAGAGCATGAGCATGTGGTAGAATGCA
TTTCCTGGGCTCCAGAAAGCTCATATTCCTCCATCTCTGAAGCAACAGGA
TCTGAGACTAAAAAAAGTGGTAAACCTGGGCCATTCTTGCTGTCTGGATC
CAGAGACAAGACTATTAAGATGTGGGATGTCAGTACTGGCATGTGCCTTA
TGACCCTCGTGGGTCATGATAACTGGGTACGTGGAGTTCTGTTCCATTCT
GGGGGGAAGTTTATTTTGAGTTGTGCTGATGACAAGACCCTACGCGTATG
GGATTACAAGAACAAGCGATGCATGAAGACCCTCAATGCGCATGAACACT
TTGTTACCTCCTTGGATTTCCACAAGACGGCACCCTATGTCGTCACTGGC
AGCGTAGATCAAACAGTAAAAGTGTGGGAGTGCCGTTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSPEFMVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAE
LDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLG
QKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWD
YETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIR
TMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREW
VRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAP
ESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVG
HDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSL
DFHKTAPYVVTGSVDQTVKVWECR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11851
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct.