cDNA Clone TRIM28

• Synonyms: KAP1; PPP1R157; RNF96; TF1B; TIF1B
• Plasmid: pCMV5-FLAG TRIM28
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG TRIM28
• Genbank: NM_005762.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCATGGCGGCCTCCGCGGCGGCAGCCTCGGCAGCAGCGGCCTCGGCCGCCTCTGGCAGCCCGGGCCCGGGCGAGGGCTCCGCTGGCGGCGAAAAGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGCCTCAGCCGCGGCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCACTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGCTGCCCTGTTTGCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCCGCCGCCGCCAACAGCTCGGGGGACGGCGGGGCGGCGGGCGACGGCACCGTGGTGGACTGTCCCGTGTGCAAGCAACAGTGCTTCTCCAAAGACATCGTGGAGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCACCGACGCCCAGGATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAGCTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGCACCAGCGGGTGAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCAGCCAAGTCTCGGGATGGTGAACGTACTGTCTATTGCAACGTACACAAGCATGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGATACTCTCACCTGCCGAGACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTTCTTAGAGGATGCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGGGGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAATCCGCCAGGTGTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGCAGATCATGAAGGAGCTGAATAAGCGGGGCCGTGTGCTGGTCAATGATGCCCAGAAGGTGACTGAGGGGCAGCAGGAGCGCCTGGAGCGGCAGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCACATTCTGCGCTTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTCTAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGGATCCCGTGGAGCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCCTGGACCAAGAGTGCCGAGGCCTTTGGCAAGATTGTGGCAGAGCGTCCTGGCACTAACTCAACAGGCCCTGCACCCATGGCCCCTCCAAGAGCCCCAGGGCCCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGAGGTGCAGGAAGGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCATGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCCTTATGCGCAAGGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTCACAGCTGACAGCCAGCCACCCGTCTTCAAGGTCTTCCCAGGCAGTACCACTGAGGACTACAACCTTATTGTTATTGAACGTGGCGCTGCCGCTGCAGCTACCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGCCCCACCCCTGGCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGCCCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTCTTGCGGAGGGTCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGCAGCACCAGCTCAGGGCTGGAGGTGGTGGCTCCTGAGGGTACCTCAGCCCCAGGTGGTGGCCCGGGAACCCTGGATGACAGTGCCACCATTTGCCGTGTCTGCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGAGTTTTGTTTCCACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTGGAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCAGCCTCAGCCTGGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCACCAGCCAACCAGCGGAAATGTGAGCGTGTACTGCTGGCCCTATTCTGTCACGAACCCTGCCGCCCCCTGCATCAGCTGGCTACCGACTCCACCTTCTCCCTGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCGTGCCCGCCTCCAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGATGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAGACGTGCAGTCCATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAACGAGGCCTTCGGTGACACCAAGTTCTCTGCTGTGCTGGTGGAGCCCCCGCCGATGAGCCTGCCTGGTGCTGGCCTGAGTTCCCAGGAGCTGTCTGGTGGCCCTGGTGATGGCCCCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSPEFMAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHS
ACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKY
TKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSD
VQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPH
GEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVP
RVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPG
AEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDG
ADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSII
GLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5-FLAG TRIM28321.41 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU11812
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No