cDNA Clone EMAPL

• Plasmid: pcDNA5-FRT/TP-GFP-TAP EMAPL
• Parent Plasmid: pcDNA5 FRT/TO GFPTAP
• Expressed: GFP-TAP EMAPL
• Genbank: AK093146
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCCACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCTCAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAACCTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCTCTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAGGCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATCCCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGAGACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCTCTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGTGTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTGTTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGATAAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGACGCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTGGGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTGGATGATTCCAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCTTCAACCCTCGTGAC
AGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTGGAATTG
GAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGAAACAGG
GTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTTGTGTTC
CTTCCGGATGGAGACATTCTCACCGGAGACTCAGAGGGGAACATTCTCAC
CTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTGGCGCCA
AAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGTTCTATC
TTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGGCGGGCG
GGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCCAGGAGG
CTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAAGGGCTT
GGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAGGGGAGA
CCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATGAGCTCT
GGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGCGGCCAC
GACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGCCTGGAG
CATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGAGTGGGG
CAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTGGACACA
GAGACCAGAGAGATCGTGTCTGATGTCATTGATGGCAATGAGCAGCTCTC
AGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGCCATTGGTTCCCATG
ACAACGTGATCTACATCTATAGTGTTTCCAGTGATGGTGCCAAATCCAGC
CGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATCACTCATCTTGACTG
GTCCAAGGATGGGAATTTCATCATGTCCAATTCTGGGGACTATGAGATTC
TTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGAAGAATCGCTATGAG
AGCCGAGACCGGGAATGGGCTACCTACACCTGTGTGCTGGGCTTTCACGT
CTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCCGCATGTACGGGGGC
CACGGCAGCCACGTGACCAGCGTCCGATTCACGCACGACGACTCGCACCT
CGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCAGTGGCGAGTGCTGG
GCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCTCTCGAACCCCCTCC
CTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGCTGCCTGGCGGGACCGA
CTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCTTCCCTGGCCCAATC
CCCCACGACTAGGGGCCGACTCTTTCCTGGACTGACTTCGAGACATTCCC
GATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCCCTGCACACACTGTT
TAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPGSSRKG
TIDAAAMELVKAALAEALRLLRLQVPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTETEVELKSSPGPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPGILRPLQPPQRADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSGSTESRGGKDPLSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIRSLEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYRPGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKPLQPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDDSNEHMLSVWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNWSGGVGVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSEGNILTWGRSPSDSKTPGRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVLSGGGRDRRLVQWGPGLVALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNALLRGDLAQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESHALAWSIDLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNEQLSVVRYSPDGLHLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDWSKDGNFIMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLGFHVYVPVRSCQGAEPHVRGPRQPRDQRPIHARRLAPRLAGRQGRQHLPVASAGRWGRGAGARHALSNPLPVPRLLPRRLIAAWRDRLARRRGPAPPCPSLAQSPTTRGRLFPGLTSRHSRSRIFLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11717
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with AK093146 CDS:; ; delta cag 108-110 (delta S36)*, ; c867t (silent G289)*, ; a1057g (N353D)*, ; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; ; * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388