cDNA Clone SASH1

• Plasmid: pCMV5-HA SASH1
• Parent Plasmid: pCMV5 HA2
• Expressed: HA SASH1
• Genbank: NM_015278.3 GI:45935384*
• Tag: HA
• Species: Human

Sequence of Insert

gcggccgcgatggaggacgcgggagcagctggcccggggccggagcctgagcccgagcccgagccggagcccgagcccgcgccggagccggaaccggagcccaagccgggtgctggcacatccgaggcgttctcccgactctggaccgacgtgatgggtatcctggacggttcactgggaaacatcgatgacctggcgcagcagtatgcagattattacaacacctgtttctccgacgtgtgcgagaggatggaggagctgcggaaacggcgggtttcccaggacctggaagtggagaaacccgatgctagccccacgtcacttcagctgcggtcccagatcgaagagtcgcttggcttctgtagcgccgtgtcaaccccagaagtggaaagaaagaaccctcttcataaatcaaactcagaagacagctctgtaggaaaaggagactggaagaagaaa
aataagtatttctggcagaacttccgaaagaaccagaaagggataatgag
acagacttcaaaaggagaagacgttggttatgttgccagtgaaataacga
tgagcgatgaggagcggattcagctaatgatgatggtcaaagaaaagatg
atcacaattgaggaagcacttgctaggctcatggaatacgaggcccagca
ccggcagtcggctgccctggaccctgctgactggccagatggttcttacc
caacgtttgatggctcatcaaactgcaattcaagagaacaatcggatgat
gagactgaggagtcggtgaagtttaagaggttacacaagctggtaaactc
cactcgcagagtcagaaagaaactaattagggtggaagaaatgaaaaaac
ccagcactgaaggtggggaggagcacgtgtttgagaattcgccggtcctg
gatgaacggtccgccctctactctggcgtgcacaagaagccccttttctt
tgatggctctcctgagaaacctcccgaagatgactcagactctctcacca
cgtctccatcctccagcagcctggacacctggggggctggccggaagttg
gtcaaaaccttcagcaaaggagagagccggggcctgattaagccccccaa
gaagatggggacattcttctcctacccagaagaagaaaaggcccagaaag
tgtcccgctccctcaccgagggggagatgaagaagggtctcgggtcccta
agccacgggagaacctgcagttttggaggatttgacttgacgaatcgctc
tctgcacgttggcagtaataattctgacccaatgggtaaagaaggagact
ttgtgtacaaagaagtcatcaaatcacctactgcctctcgcatctctctt
gggaaaaaggtgaaatcagtgaaagagacgatgagaaagagaatgtctaa
aaaatacagcagctctgtctctgagcaggactcgggccttgatggaatgc
ctggctcccctccgccttcacagcccgaccccgaacacttggacaagccc
aagctcaaggccgggggttctgtagaaagtcttcgcagttctctcagtgg
gcagagctccatgagcggtcaaacagtgagcaccactgattcctcaacca
gcaaccgggaaagcgtcaagtcggaagatggggatgacgaagagccgcct
taccgaggcccgttctgcgggcgtgccagggtgcacaccgacttcacccc
cagtccctatgacacagactcactcaagctcaagaaaggagatatcatcg
atataatcagcaagccacccatggggacctggatgggcctgctgaacaac
aaagtcggcacgttcaagttcatctacgtggacgtgctcagtgaagacga
ggagaaacccaaacgccccaccaggaggcgtcggaaaggacgaccacccc
agcccaagtctgtggaggatctcctggatcggattaacctaaaagagcac
atgcccactttcctgttcaatggatatgaagatttggacacctttaagct
gctggaggaggaagacttggatgagttaaatatcagggacccggaacaca
gagctgttctcttgacagcagtggagctgttacaagagtatgacagtaac
agcgaccagtcaggatcccaggagaagctgctcgttgacagccagggcct
gagtggatgctcaccccgagactcaggatgctacgaaagcagtgagaacc
tggaaaacggcaagactcggaaagctagcctcctatctgccaagtcatcc
accgagcccagcttgaagtcttttagcagaaaccagttgggcaattaccc
aacattgcctttaatgaaatcaggggatgcactgaagcagggacaggagg
agggcaggctgggtggtggccttgccccagacacgtccaagagctgtgac
ccacctggtgtgactggtttgaataaaaaccgaagaagcctcccagtttc
catctgccggagctgtgagaccctggagggcccccagactgtggacactt
ggccccgatcccattccctggatgaccttcaagtggagcctggtgctgag
caagacgtgcctaccgaggtgacagaaccgccccctcagattgtacctga
agtgccacagaagacgaccgcctcttccacgaaggcccagcccctggagc
gagactctgctgtcgacaatgcattgctactgacccaaagcaagagattt
tctgaacctcagaaattgacaactaagaaactggagggctcaatcgcagc
ctctggtcgcggcctgtcaccccctcagtgtttgcccagaaactatgatg
ctcagcctcctggagctaaacacggtttagcaaggacgcctctggagggc
cacagaaaaggacacgagtttgaaggaacacaccatcccctgggcaccaa
agaaggggtagatgctgagcagagaatgcagcccaaaattccatcacagc
ctccacctgttcctgccaaaaagagcagagaacgccttgctaacggactc
caccctgttcccatgggccccagtggggccctccccagtcccgatgcgcc
atgcctgccagtgaaaaggggcagccccgccagccccaccagccctagcg
actgtcccccagcactggctcccaggcctctctcagggcaggcgcctggc
agcccaccaagcacaaggccgcccccctggctctcagagctccccgagaa
cacaagcctccaggagcacggtgtgaagctgggcccggctttgaccagga
aggtctcctgtgcccggggagtggatctagaaacgctcactgaaaacaag
ctgcacgctgaaggcatcgatctcacggaggagccgtattctgataagca
tggccgctgtgggattcctgaagccctggtgcagagatacgcagaggact
tggatcagcccgagcgggacgtcgccgccaacatggaccagatccgggtg
aagcagcttcggaagcagcaccgcatggcgattccaagtggtggactcac
ggaaatctgccgaaagcccgtctctcctgggtgcatttcgtctgtgtcag
attggctcatttccatcggtctgcccatgtacgccggcaccctctccacc
gcgggcttcagcacactgagccaagtgccttctctgtctcacacttgcct
tcaggaggccggcatcacagaggagagacacataagaaagctcctatctg
cagccagactcttcaaactgccgccaggccctgaggccatgtagcggccgc

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMEDAGAAGPGPEPEPEPEPEPEPAPEP
EPEPKPGAGTSEAFSRLWTDVMGILDGSLGNIDDLAQQYADYYNTCFSDV
CERMEELRKRRVSQDLEVEKPDASPTSLQLRSQIEESLGFCSAVSTPEVE
RKNPLHKSNSEDSSVGKGDWKKKNKYFWQNFRKNQKGIMRQTSKGEDVGY
VASEITMSDEERIQLMMMVKEKMITIEEALARLMEYEAQHRQSAALDPAD
WPDGSYPTFDGSSNCNSREQSDDETEESVKFKRLHKLVNSTRRVRKKLIR
VEEMKKPSTEGGEEHVFENSPVLDERSALYSGVHKKPLFFDGSPEKPPED
DSDSLTTSPSSSSLDTWGAGRKLVKTFSKGESRGLIKPPKKMGTFFSYPE
EEKAQKVSRSLTEGEMKKGLGSLSHGRTCSFGGFDLTNRSLHVGSNNSDP
MGKEGDFVYKEVIKSPTASRISLGKKVKSVKETMRKRMSKKYSSSVSEQD
SGLDGMPGSPPPSQPDPEHLDKPKLKAGGSVESLRSSLSGQSSMSGQTVS
TTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKL
KKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLSEDEEKPKRPTRRR
RKGRPPQPKSVEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELN
IRDPEHRAVLLTAVELLQEYDSNSDQSGSQEKLLVDSQGLSGCSPRDSGC
YESSENLENGKTRKASLLSAKSSTEPSLKSFSRNQLGNYPTLPLMKSGDA
LKQGQEEGRLGGGLAPDTSKSCDPPGVTGLNKNRRSLPVSICRSCETLEG
PQTVDTWPRSHSLDDLQVEPGAEQDVPTEVTEPPPQIVPEVPQKTTASST
KAQPLERDSAVDNALLLTQSKRFSEPQKLTTKKLEGSIAASGRGLSPPQC
LPRNYDAQPPGAKHGLARTPLEGHRKGHEFEGTHHPLGTKEGVDAEQRMQ
PKIPSQPPPVPAKKSRERLANGLHPVPMGPSGALPSPDAPCLPVKRGSPA
SPTSPSDCPPALAPRPLSGQAPGSPPSTRPPPWLSELPENTSLQEHGVKL
GPALTRKVSCARGVDLETLTENKLHAEGIDLTEEPYSDKHGRCGIPEALV
QRYAEDLDQPERDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPG
CISSVSDWLISIGLPMYAGTLSTAGFSTLSQVPSLSHTCLQEAGITEERH
IRKLLSAARLFKLPPGPEAM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11637
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015278.3 CDS:; a492g (sil); a632t (K211M) a2651g (Q884R); Coding differences present in other published seqs (match AAH63279.1); ; Estimated MW of expressed protein : 139045 Daltons