cDNA Clone Mms22 (yeast)

• Plasmid: pCMV5 Mms22-FLAG
• Parent Plasmid: pCMV5 C-FLAG
• Tag: FLAG
• Species: Yeast

Sequence of Insert

GCGGCCGCCACCATGGATGTTGATGAACCGAATCCTATTGTGATATCAGATTCTGAGGCCACTGATGAGGAAATTAGTATCATATACGAACCCGAATTCAATGAAAATTATTTATGGGCAGAGGAAAACGTCCAAGAAGCAAGCAGATCACAGAAAATTGTCACTGAGAGGTTGAGCCTGGATTCCACTGCTGGAGAATCTTGTACTCCTTCCGTAGTAACTGATACACAAGTTACTACGGGGCTTCGGTGGTCTTTGAGGAAGAGGAAGGCTATTCAAAAGATGCCATACAGTTTAGAACGCATAAAGCATAGGCAGTTGCTAGAGGGCTATGATATCTCTAGTTTTGACAGCATTTCGAATCAGTTGACCTTACCGAAGAACGCTTCTACAGTGATTCACTCAAATGATATTTTACTTACAAAGAGAACTGGTAAACCCTTAGATGAGCAGAAAGATGTTACAATCGACTCCATTAAACCCGAAAACAGCTCGGTTCAATCTCAAAGATATGATAGTGATGAAGAAATACCAAAGAAAAGGCATCGTACTTTTAAAGACCTCGACCAGGATATAGTATTTCAATCAGGTGATTCTACGGAAGATGAACAA
GACTTGGCTAGTACAAATTTACAGAATACTCAAAATGACGAAGTAATATT
TAGAGGAAGAGTATTGAATGTAAGAACTGGTTACCGTGGGGTCCTGCCAA
GAGTTGCTTGGGAAAAGTCGTTGCAAAAGCAGCAGTCTTCAAAAGTCACG
AAAAGAAAAACTCAATTATTGAACCATAAGGGTGTGGCTAAGAGGAAAAT
GAACAGAAGCGCCCATATTGAAGATGAAGAACAGAACTTATTGAACGATC
TTATTGCACCTGATGACGAACTGGATATCGAGGAAAACGCCCCTCCAGAT
ATATACTTAGGAAATCTTCCAGAGGATCGTGAGGCTAACGAAAAGGAATT
AAAGGAATTGCAAGAATATTATGAGAGCAAGTATAGTGAAGACGCACAAT
CGGCTGGAACTTCTGGTTTTAATTTAAATGAGGAATACCGCAATGAACCA
GTGTATGAGCTGGAATATGACGGACCGGGGAGCTGCATATCACATGTGTC
TTATAAAGACCAGCCTATTATTTATTTGAATTCACGGCATTCAGATAGCG
GATTTAGTGAGCAATACAATATATCTGCCGAAGATAATCAAAGTGTAATT
TCCCTAGATGCTGCTGAAGAGCATAACGATGGTATAATTGACAAGATGTT
GGTTAAACCTAAAAGAATTAAAGCAACCAATGATGCCAACTTTTTGAATA
CAAAAAGTAAGAGAGTGAGAAGATATAAGTACAAATATCGAAATTCTTGT
CTGGCCCCAAGTACAAAAGCTATCAAAGTCGGAAAACGGTCAGCGCATAA
ATCTCATTTGGCAGCTAATAATCCAGTTTCATTTGTTTCTAAGAAGAATC
ATGTAATTGATGATTATTTTTTTGAAGAATTAGAATCTCAATCCCTAGAA
CAGGATGACAGCTCTTCTCTCAAGCCACAGAAAAAAAGAAGGAAGAAGAA
AGCACCTATATACTCAAGTTTCTCCGCTGATCTTGAATCGAGACGAAAAC
CAGTTTTCAATACTGTTGTAGAGGTTCCCACGAACCGATACGCTTTCACA
AAGCCAAATGTACGTAATAGGGACAGCATTAATCATGACATGGAATTTGA
GGAAGAAGACTCCAATCAAGAGCTTGGACCAATAATGGTCGTACTTGACT
CAATTTTACTAAAAAAACCTTTTGAGCCACCTAATTTCTTCAAGATCCAG
TTGTCAGACAAGAGTTTCTTGCTATCAAAATTGAATCCTGCAGATATAGC
TACATCATTACAGAAAATATTCAGAGTAATCATTGATAAAGGTATCACTG
ATACTGAACTAGTTCACTTTAACGAAAGCCTTATTGCTTTTTTGGTGCAC
CTTGACATGCCTGAACTATTCGACTTAATAGGTGAATTTCATCGTGAATT
TAGATCGAAGGTCAATAGCTTGAGGAAAAAAGCTAAACCAATCCATTTTT
TTCAAATTGCGGCATGCCAATTAATGTTTCTAGAAATATCAAGATATAAC
AAAATTTCAGCCGCTGCAAAATTCGACATGGATGTCAAACTCTTAGATCA
TATTGTGTCTTTCTTCAAATTACTTTCTGTCTGTTATGATTCTGTTATGA
AAAATCCAATGCAATATCTGTACACAAGTTATTATATTCTCTCTGCTGTC
GTTGATGTCATACATAAAAAGGAAGCTTTGTGGGATTTATTTCAAAAGCA
CCCCTTTTCGCCTCATATTTCACTGTTGCTGGTCAACATATTTCCTACGA
AAGTCTGCCGCTGGCAGGTTTTGAGATTAGATTCTGAATTCCAACCCTTG
AGTTCGGCATTTCGGTTCATCAATTATTGCATTGAGACTTGTAATTGGAA
TGTTACAAATTCTTTGATTCTTTCTCTCGATCGCATATTCAAACGGAGAA
GATTTTCGGACTTTGAAGAAGAGTCTGATTTGTCACAAAACAATAAGATA
ATTTATCCTCCAACGAATCAGCTTACGAGTCGATTAATGTTTAACAGATA
TTTGCATCTACTGACGTTGTGTGAATTGTCTAGTTCTGATACACAACGTG
TGATACCGATGGGTGATATTTCTATGAATGATTCTCTATCTGTTTTGAAG
AACCGCCTCAACCTACTAATTGTTCTAGCTACACGTTTTGATTTAAACTT
AGAGAAGCGCTTCCAAGAGCTAACACGACCGTTATATAGTAAGGAATATT
TAAACTTGCATACACAGAACACAGTCAGGACGATAACAACATTGATCATG
CAGGCCAGCCTTTCATTCCTGGAAATCAGTCGAATCAAAAATCATCCATT
CAGTGGAAAATTTATTGCGTCATTATTTGATAAACTAGTACTTCAACAAC
CTTCCATATCGGGAGTAACAGAAAATTTTTTGAAAGAATTCACAAATTTA
GTCTCCAAAATGAAAAGAAAAAGCGTGAGTATGCTCAAATTTCTGTATCC
TTCACTGGTGGCCATGTCTCAAGAAAATATATTCGAGTCGTCTTTTTTTT
TATTATTACAGGTATATTTGAAGAGCTTAGATGTGCTGGGTCCAACTTGG
GTGCAAAATTATCTTTTTCAGTTTATAAAGAGCAAGGCTCAAGAAAATGA
AAGATGGATAGAATGCTATTGTCAGATCGGAAAATTCCTCGTAGATTCTG
GTATATTTACCTGGTGGACTTTCTTTACCTATAACGGCTTAGATGCTGCA
TTACACTTTCAGCTTGCATTTCACTCCCTTATTATCGATTTCTGTGATAC
AGACTCTTTTGAATTACTGAAAAAACCGCTTTATTCCATTGCTTCTGATT
TGCTGCTCATTTCCAAAGATGACGCTTTCTATCACTTTCTTTCCAATCTG
TTGAAGAGGGCACATATCATAGTAGCAGATTTGAAACCTGTTTCCGATGA
GAATGAACTCCTGCGATTGGCTTATATTTTTTCTAAAGCATTGAAAAAAA
ATGCATATCAAGATCTATTAGCTGTTTTCTTATCCCTGGCGAAAAAACAT
TACGATGAAGGGGACATCAGTAGAAATTTCTTGGCAAAATATCTCGAATT
TCTTAACAAAAATTGCTTGACTGAGCTAAGAAATAATCAACTCTTCATCA
GTCTAAGAAGGGAACTTGGTATTTCAAGTGATGAAGACGAAAAATGTGCG
TTCTGGGACTCATTCAATGAAGCAGGTGATATACTTTCCAAAGCTGCATT
TGTTGAAACCGGTATTGTTCAAGCCTGTTGCACTGGGAATGAAATTGATG
GATATCTTGACAATTTAAGCACTTTATTCACCTCCACCATGTTAGAGAGC
CCTTTTGCATTTTTTTCTGACCTGGTGATAGCACACATATTTGAGAACAG
ACCTTTTTTCGACGTGAATATCAAGAATTTTCTTCTATCTCACTTCATAG
ACCTTTTCAACAAGGTTTTAAAAATGAAATTCGAGCAGGTATCTCCTGAT
GAATTTGCTGAACTTTGCAAAGTATACAGGGCGTTATGTATTGAGTGCGC
AACAGACGACACCTTTAATAGTAATAGTGATTTGATCGCCGCAAAAGATG
CGTTTTTGGTATCTGTGTTGAGAATCGCAGATGGGTTTTGGGAGCATGAC
AAACTTTTGCAGCTAAGGATGCTTGATAGTAATATGAACATACCTAATCA
GATTCCTCATACTACTTTGCAAAGTTCGCTATCTGCGATTGTAATAAAGA
TAATAGAAAGTAATATAGGAAAAATTGAGGCGTCTGAACCTTTCAAAACC
TTCAAGAATACGGGATCC

Amino Acid Sequence

MDVDEPNPIVISDSEATDEEISIIYEPEFNENYLWAEENVQEASRSQKIV
TERLSLDSTAGESCTPSVVTDTQVTTGLRWSLRKRKAIQKMPYSLERIKH
RQLLEGYDISSFDSISNQLTLPKNASTVIHSNDILLTKRTGKPLDEQKDV
TIDSIKPENSSVQSQRYDSDEEIPKKRHRTFKDLDQDIVFQSGDSTEDEQ
DLASTNLQNTQNDEVIFRGRVLNVRTGYRGVLPRVAWEKSLQKQQSSKVT
KRKTQLLNHKGVAKRKMNRSAHIEDEEQNLLNDLIAPDDELDIEENAPPD
IYLGNLPEDREANEKELKELQEYYESKYSEDAQSAGTSGFNLNEEYRNEP
VYELEYDGPGSCISHVSYKDQPIIYLNSRHSDSGFSEQYNISAEDNQSVI
SLDAAEEHNDGIIDKMLVKPKRIKATNDANFLNTKSKRVRRYKYKYRNSC
LAPSTKAIKVGKRSAHKSHLAANNPVSFVSKKNHVIDDYFFEELESQSLE
QDDSSSLKPQKKRRKKKAPIYSSFSADLESRRKPVFNTVVEVPTNRYAFT
KPNVRNRDSINHDMEFEEEDSNQELGPIMVVLDSILLKKPFEPPNFFKIQ
LSDKSFLLSKLNPADIATSLQKIFRVIIDKGITDTELVHFNESLIAFLVH
LDMPELFDLIGEFHREFRSKVNSLRKKAKPIHFFQIAACQLMFLEISRYN
KISAAAKFDMDVKLLDHIVSFFKLLSVCYDSVMKNPMQYLYTSYYILSAV
VDVIHKKEALWDLFQKHPFSPHISLLLVNIFPTKVCRWQVLRLDSEFQPL
SSAFRFINYCIETCNWNVTNSLILSLDRIFKRRRFSDFEEESDLSQNNKI
IYPPTNQLTSRLMFNRYLHLLTLCELSSSDTQRVIPMGDISMNDSLSVLK
NRLNLLIVLATRFDLNLEKRFQELTRPLYSKEYLNLHTQNTVRTITTLIM
QASLSFLEISRIKNHPFSGKFIASLFDKLVLQQPSISGVTENFLKEFTNL
VSKMKRKSVSMLKFLYPSLVAMSQENIFESSFFLLLQVYLKSLDVLGPTW
VQNYLFQFIKSKAQENERWIECYCQIGKFLVDSGIFTWWTFFTYNGLDAA
LHFQLAFHSLIIDFCDTDSFELLKKPLYSIASDLLLISKDDAFYHFLSNL
LKRAHIIVADLKPVSDENELLRLAYIFSKALKKNAYQDLLAVFLSLAKKH
YDEGDISRNFLAKYLEFLNKNCLTELRNNQLFISLRRELGISSDEDEKCA
FWDSFNEAGDILSKAAFVETGIVQACCTGNEIDGYLDNLSTLFTSTMLES
PFAFFSDLVIAHIFENRPFFDVNIKNFLLSHFIDLFNKVLKMKFEQVSPD
EFAELCKVYRALCIECATDDTFNSNSDLIAAKDAFLVSVLRIADGFWEHD
KLLQLRMLDSNMNIPNQIPHTTLQSSLSAIVIKIIESNIGKIEASEPFKT
FKNTGSGTDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11618
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee