cDNA Clone TBC1D1

• Plasmid: pGEX6P-TBC1D1 1-796
• Parent Plasmid: pGEX6P3
• Expressed: GST-TBC1D1 1-796
• Genbank: NM_015173
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAA
CGAGGTCTCGGTGGATTTTGGCCTGCAGCTGGTGGGCTCCCTGCCTGTGC
ATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGTGCGA
AGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCG
GCTTTGCGTTTCACCCTCTGGACTGAGATGTGAACCTGAGCCAGGGAGAA
GTCAACAGTGGGATCCCCTGATCTATTCCAGCATCTTTGAGTGCAAGCCT
CAGCGTGTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGC
TTGTCTGATTAAGGAAGACGCTGTCCACCGGCAGAGTATCTGCTATGTGT
TCAAAGCCGATGATCAAACAAAAGTGCCTGAGATCATCAGCTCCATCCGT
CAGGCGGGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCGAGTT
CGACGACACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGA
CGGTGGCGCACAAGAAGGCTCCGCCGGCCCTGATCGACGAGTGCATCGAG
AAGTTCAATCACGTCAGCGGCAGCCGGGGGTCCGAGAGCCCCCGCCCCAA
CCCGCCCCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCA
TGCGCAAGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAG
GAGCTGCAGGATGGGGGCCTCCGAAGCAGCGGCTTCTTCAGCTCCTTCGA
GGAGAGCGACATTGAGAACCACCTCATTAGCGGACACAATATTGTGCAGC
CCACAGATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCT
GAAGTTTACCTCATCAGTCCTGACACCAAAAAAATAGCATTGGAGAAAAA
TTTTAAGGAGATATCCTTTTGCTCTCAGGGCATCAGACACGTGGACCACT
TTGGGTTTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTC
TGTTACGTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGAT
GACCCTGAAACAGGCCTTCACGGTGGCCGCAGTGCAGCAGACAGCTAAGG
CGCCAGCCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAAGCTC
TGTGAGAGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCA
AAAGCACCTGACGACATTAACAAATCAGGAGCAGGCGACTATTTTTGAAG
AGGTTCAGAAATTGAGACCGAGAAATGAGCAGCGAGAGAATGAATTGATT
ATTTCTTTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCA
TATTGGGGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAA
GTGAATTACCACCCAGTGCCACTCGATTTAGGCTAGATATGCTGAAAAAC
AAAGCAAAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCCGGGG
TAATAAAGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATA
GCTCCCTGTCTAGTACATTAAGTAACACCAGCAAAGAGCCATCTGTGTGT
GAAAAGGAGGCCTTGCCCATCTCTGAGAGCTCCTTTAAGCTCCTCGGCTC
CTCGGAGGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAG
CTCCGCTGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGT
CACTTCCCCATCGAATGCCAGGAACCTCCACAACCTGCCCGGGGGTCCCC
GGGGGTTTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACAGAGA
CGCCTCATGAACGAAAGGACTTTGAATCCAAAGCAAACCATCTTGGTGAT
TCTGGTGGGACTCCTGTGAAGACCCGGAGGCATTCCTGGAGGCAGCAGAT
ATTCCTCCGAGTAGCCACCCCGCAGAAGGCGTGCGATTCTTCCAGCAGAT
ATGAAGATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAA
CCAGTTTGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAG
GACATCTCGTGAGCTCCGAGAGCTGTGGCAAAAGGCTATTCTTCAACAGA
TACTGCTGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCCTCTGAA
AATGATTTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCC
CTGTCTTAAAGAAGTAACTACAGTGTGGGAAAAGATGCTTAGCACTCCAG
GAAGATCAAAAATTAAGTTTGACATGGAAAAAATGCACTCGGCTGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMEPITFTARKHLL
SNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQ
VRLCVSPSGLRCEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSY
FACLIKEDAVHRQSICYVFKADDQTKVPEIISSIRQAGKIARQEELHCPS
EFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSESPR
PNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSS
FEESDIENHLISGHNIVQPTDIEENRTMLFTIGQSEVYLISPDTKKIALE
KNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCYVFQCTNEALVDEI
MMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLE
LQKHLTTLTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEH
IHIGEMKQTSQMAAENIGSELPPSATRFRLDMLKNKAKRSLTESLESILS
RGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSFKLL
GSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARG
SPGVSQRKLMRYHSVSTETPHERKDFESKANHLGDSGGTPVKTRRHSWRQ
QIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPVCEDGPFGPPPEEK
KRTSRELRELWQKAILQQILLLRMEKENQKLQASENDLLNKRLKLDYEEI
TPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSAVDSSGRIVTD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11611
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422); ; Estimated MW of expressed protein : 118656 Daltons