cDNA Clone USE1

• Plasmid: pGEX6P-2-USE1
• Parent Plasmid: pGEX6P2
• Expressed: GST-USE1
• Genbank: NM_023079.3 GI:150417998
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatggcggagagtccgactgaggaggcggcaacggcgggcgccggggcggcgggccccggggcgagcagcgttgctggtgttgttggcgttagcggcagcggcggcgggttcgggccgcctttcctgccggatgtgtgggcggcggcggcggcagcgggcggggccgggggcccggggagcggcctggctccgctgcccgggctcccgccctcagccgctgcccacggggccgcgctgcttagccactgggaccccacgctcagctccgactgggacggcgagcgcaccgcgccgcagtgt
ctactccggatcaagcgggatatcatgtccatttataaggagcctcctcc
aggaatgttcgttgtacctgatactgttgacatgactaagattcatgcat
tgatcacaggcccatttgacactccttatgaagggggtttcttcctgttc
gtgtttcggtgtccgcccgactatcccatccacccacctcgggtcaaact
gatgacaacgggcaataacacagtgaggtttaaccccaacttctaccgca
atgggaaagtctgcttgagtattctaggtacatggactggacctgcctgg
agcccagcccagagcatctcctcagtgctcatctctatccagtccctgat
gactgagaacccctatcacaatgagcccggctttgaacaggagagacatc
caggagacagcaaaaactataatgaatgtatccggcacgagaccatcaga
gttgcagtctgtgacatgatggaaggaaagtgtccctgtcctgaacccct
acgaggggtgatggagaagtcctttctggagtattacgacttctatgagg
tggcctgcaaagatcgcctgcaccttcaaggccaaactatgcaggaccct
tttggagagaagcggggccactttgactaccagtccctcttgatgcgcct
gggactgatacgtcagaaagtgctggagaggctccataatgagaatgcag
aaatggactctgatagcagttcatctgggacagagacagaccttcatggg
agcctgagggtttagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAESPTEEAATAGAGAAGP
GASSVAGVVGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLP
PSAAAHGAALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGM
FVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMT
TGNNTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTE
NPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRG
VMEKSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGL
IRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11585
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; Sequence difference compared with NM_023079.3 CDS: ; c846t (silent); ; Estimated MW of expressed protein : 65032 Daltons