cDNA Clone TBC1D1

• Plasmid: pECFPC1 TBC1D1
• Parent Plasmid: pECFPC1
• Expressed: CFP-TBC1D1
• Genbank: NM_015173
• Tag: CFP
• Species: Human

Sequence of Insert

GTCGACATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAACGAGGTCTCGGTGGATTTTGGCCTGCAGCTGGTGGGCTCCCTGCCTGTGCATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGTGCGAAGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCGGCTTTGCGTTTCACCCTCTGGACTGAGATGTGAACCTGAGCCAGGGAGAAGTCAACAGTGGGATCCCCTGATCTATTCCAGCATCTTTGAGTGCAAGCCTCAGCGT
GTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGCTTGTCT
GATTAAGGAAGACGCTGTCCACCGGCAGAGTATCTGCTATGTGTTCAAAG
CCGATGATCAAACAAAAGTGCCTGAGATCATCAGCTCCATCCGTCAGGCG
GGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCGAGTTCGACGA
CACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGACGGTGG
CGCACAAGAAGGCTCCGCCGGCCCTGATCGACGAGTGCATCGAGAAGTTC
AATCACGTCAGCGGCAGCCGGGGGTCCGAGAGCCCCCGCCCCAACCCGCC
CCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCATGCGCA
AGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAGGAGCTG
CAGGATGGGGGCCTCCGAAGCAGCGGCTTCTTCAGCTCCTTCGAGGAGAG
CGACATTGAGAACCACCTCATTAGCGGACACAATATTGTGCAGCCCACAG
ATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCTGAAGTT
TACCTCATCAGTCCTGACACCAAAAAAATAGCATTGGAGAAAAATTTTAA
GGAGATATCCTTTTGCTCTCAGGGCATCAGACACGTGGACCACTTTGGGT
TTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTCTGTTAC
GTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGATGACCCT
GAAACAGGCCTTCACGGTGGCCGCAGTGCAGCAGACAGCTAAGGCGCCAG
CCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAAGCTCTGTGAG
AGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCAAAAGCA
CCTGACGACATTAACAAATCAGGAGCAGGCGACTATTTTTGAAGAGGTTC
AGAAATTGAGACCGAGAAATGAGCAGCGAGAGAATGAATTGATTATTTCT
TTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCATATTGG
GGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAAGTGAAT
TACCACCCAGTGCCACTCGATTTAGGCTAGATATGCTGAAAAACAAAGCA
AAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCCGGGGTAATAA
AGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATAGCTCCC
TGTCTAGTACATTAAGTAACACCAGCAAAGAGCCATCTGTGTGTGAAAAG
GAGGCCTTGCCCATCTCTGAGAGCTCCTTTAAGCTCCTCGGCTCCTCGGA
GGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAGCTCCGC
TGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGTCACTTC
CCCATCGAATGCCAGGAACCTCCACAACCTGCCCGGGGGTCCCCGGGGGT
TTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACAGAGACGCCTC
ATGAACGAAAGGACTTTGAATCCAAAGCAAACCATCTTGGTGATTCTGGT
GGGACTCCTGTGAAGACCCGGAGGCATTCCTGGAGGCAGCAGATATTCCT
CCGAGTAGCCACCCCGCAGAAGGCGTGCGATTCTTCCAGCAGATATGAAG
ATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAACCAGTT
TGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAGGACATC
TCGTGAGCTCCGAGAGCTGTGGCAAAAGGCTATTCTTCAACAGATACTGC
TGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCCTCTGAAAATGAT
TTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCCCTGTCT
TAAAGAAGTAACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGAT
CAAAAATTAAGTTTGACATGGAAAAAATGCACTCGGCTGTTGGGCAAGGT
GTGCCACGTCATCACCGAGGTGAAATCTGGAAATTTCTAGCTGAGCAATT
CCACCTTAAACACCAGTTTCCCAGCAAACAGCAGCCAAAGGATGTGCCAT
ACAAAGAACTCTTAAAGCAGCTGACTTCCCAGCAGCATGCGATTCTTATT
GACCTTGGGCGAACCTTTCCTACACACCCATACTTCTCTGCCCAGCTTGG
AGCAGGACAGCTATCGCTTTACAACATTTTGAAGGCCTACTCACTTCTAG
ACCAGGAAGTGGGATATTGCCAAGGTCTCAGCTTTGTAGCAGGCATTTTG
CTTCTTCATATGAGTGAGGAAGAGGCGTTTAAAATGCTCAAGTTTCTGAT
GTTTGACATGGGGCTGCGGAAACAGTATCGGCCAGACATGATTATTTTAC
AGATCCAGATGTACCAGCTCTCGAGGTTGCTTCATGATTACCACAGAGAC
CTCTACAATCACCTGGAGGAGCACGAGATCGGCCCCAGCCTCTACGCTGC
CCCCTGGTTCCTCACCATGTTTGCCTCACAGTTCCCGCTGGGATTCGTAG
CCAGAGTCTTTGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAA
GTGGCTTTAAGTCTGTTGGGAAGCCATAAGCCCTTGATTCTGCAGCATGA
AAACCTAGAAACCATAGTTGACTTTATAAAAAGCACGCTACCCAACCTTG
GCTTGGTACAGATGGAAAAGACCATCAATCAGGTATTTGAAATGGACATC
GCTAAACAGTTACAAGCTTATGAAGTTGAGTACCACGTCCTTCAAGAAGA
ACTTATCGATTCCTCTCCTCTCAGTGACAACCAAAGAATGGATAAATTAG
AGAAAACCAACAGCAGCTTACGCAAACAGAACCTTGACCTCCTTGAACAG
TTGCAGGTGGCAAATGGTAGGATCCAAAGCCTTGAGGCCACCATTGAGAA
GCTCCTGAGCAGTGAGAGCAAGCTGAAGCAGGCCATGCTTACCTTAGAAC
TGGAGCGGTCGGCCCTGCTGCAGACGGTGGAGGAGCTGCGGCGGCGGAGC
GCAGAGCCCAGCGACCGGGAGCCTGAGTGCACGCAGCCCGAGCCCACGGG
CGACTGAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDMEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAE
VRRLSRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIYSSIFEC
KPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKVPEIISS
IRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDEC
IEKFNHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAF
RKELQDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRTMLFTIG
QSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFH
FVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLH
KLCERIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNEQRENE
LIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATRFRLDML
KNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPS
VCEKEALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANT
LSHFPIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFESKANHL
GDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGELPPRSP
LEPVCEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKENQKLQA
SENDLLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSA
VGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQH
AILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFV
AGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHD
YHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTE
VIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVF
EMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLD
LLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEEL
RRRSAEPSDREPECTQPEPTGD*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU11577
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422)