cDNA Clone RFA1 (yeast)

• Plasmid: pRS415 Pro-RFA1-6His-cMyc
• Parent Plasmid: pRS415
• Genbank: EF059167.1 GI:116616506
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCTGGTTTTATCATTGATTCTACCGATTTTCTTTAATTTTGCAATG
TGTTCATCCTTTTTGAACTTTCTCTGATATTCAAATGACTTGTTCAAACG
ATCGTTGACAATTTTAGATAGTTTGGCGCCCCGCTCTAATGTTATTCCCT
CCATAATCTCATCTCCCTCTTGTCTTATTAATTTCACATCGGCAAGATTG
TTCAAATGCAGCCACAGCACATCTTCTAACATAAGCCTCAAAGGTACCGA
CAAAAATACATTTTGCTGTGCTGCAGTAGGCAAAGTTCCAGATAACACAC
CACAAATACCCCATTTCCTTAGAAGTTTGATGTGATCTATGTCAAATACT
AGCGGTGGCATAAAAACCTCCTCGCCTTTTGCCGTTCTTTTTGCATATAT
GTTAATAGCTATGCGCGTACCACCTTCTTCAGCCATATACGAAAGGATCT
ATGCTAATTGGGTGGATTTCTTATCGCATCACTTTCTTATTCTCTAACTC
TTCTTCGCTGCTCAATAAAATATCTCTGCTTGGAAAAATGTCACGCGTAA
AAATTAGACGAGGCGACAAAAGACGCGTGAACTAACATAAAGGTTTACTT
GAACAAATGTTATCACTTTAAGTTCTACACGTAAAACAAAAACTATTCAC
TCTTCTTATTACGGTAAAGGCGAAACCAGCAAGAAGACCAGATTATACTT
ACAAGAGATGAGCAGTGTTCAACTTTCGAGGGGCGATTTTCATAGCATCT
TCACCAATAAGCAAAGGTACGATAATCCCACCGGTGGCGTTTATCAAGTT
TATAACACCAGGAAATCTGATGGGGCTAACAGCAACAGAAAGAATTTGAT
CATGATTTCCGATGGTATTTACCATATGAAGGCTCTGTTGAGAAACCAAG
CTGCATCCAAGTTCCAGTCAATGGAACTACAAAGGGGTGATATCATTCGC
GTGATAATTGCAGAACCTGCTATTGTCAGGGAAAGAAAGAAATACGTTCT
TTTAGTAGATGACTTTGAGTTGGTCCAGTCGCGTGCTGATATGGTCAACC
AAACTAGTACTTTTTTGGATAACTATTTCTCAGAGCATCCAAATGAAACC
TTAAAAGACGAAGATATAACTGACAGTGGTAATGTTGCCAATCAAACAAA
CGCCAGCAATGCTGGTGTCCCTGATATGCTGCATTCAAACTCAAACTTGA
ATGCAAATGAGAGAAAATTCGCCAATGAAAACCCTAATTCGCAAAAAACC
AGACCAATTTTTGCCATCGAACAACTGTCTCCATACCAAAACGTTTGGAC
TATCAAAGCAAGAGTTTCCTACAAGGGAGAAATTAAAACGTGGCACAATC
AAAGAGGTGATGGTAAACTATTCAATGTCAACTTCTTGGATACCTCTGGA
GAAATCCGAGCCACGGCGTTTAATGATTTTGCTACAAAATTTAACGAAAT
TTTACAAGAAGGCAAAGTATACTATGTATCAAAGGCAAAACTCCAACCAG
CTAAGCCCCAATTTACTAATCTAACACACCCTTATGAACTGAATTTGGAT
AGAGACACTGTTATAGAAGAATGTTTCGATGAAAGTAATGTTCCGAAAAC
CCATTTCAATTTCATCAAACTAGATGCTATTCAGAACCAGGAAGTAAATT
CCAACGTAGACGTCCTCGGTATTATCCAAACTATAAACCCACATTTTGAG
CTAACTTCAAGGGCTGGGAAGAAATTCGATCGTCGTGACATCACAATTGT
TGACGACTCTGGGTTTTCTATCTCTGTTGGCCTATGGAATCAGCAAGCCC
TTGATTTCAACCTTCCTGAAGGTTCTGTTGCTGCCATTAAAGGTGTTCGT
GTGACGGATTTTGGTGGCAAATCTTTGTCTATGGGATTTTCTAGTACCCT
GATTCCGAATCCAGAAATTCCTGAGGCATATGCCTTAAAGGGTTGGTATG
ATTCCAAGGGCCGCAACGCAAACTTCATCACTTTAAAGCAAGAACCCGGT
ATGGGTGGTCAATCGGCTGCTAGCTTAACAAAATTCATTGCTCAGCGTAT
TACTATTGCTAGAGCTCAAGCTGAAAATCTAGGAAGAAGCGAGAAAGGTG
ACTTTTTTAGTGTTAAAGCTGCTATAAGTTTCTTAAAAGTTGATAATTTT
GCATATCCTGCCTGTTCTAATGAGAATTGTAATAAGAAAGTTCTGGAACA
GCCTGATGGTACTTGGAGATGTGAGAAGTGCGACACCAATAATGCAAGGC
CAAATTGGAGATACATCTTGACAATATCAATTATTGACGAAACCAATCAA
CTATGGCTCACTTTATTTGACGACCAAGCTAAACAATTATTGGGTGTTGA
TGCTAATACATTAATGTCTTTGAAGGAAGAAGACCCCAACGAATTCACAA
AAATTACTCAAAGTATCCAAATGAACGAATATGACTTTAGGATTAGAGCG
CGTGAGGATACATACAATGATCAAAGCAGAATTAGATATACCGTTGCTAA
CCTACACAGCTTGAATTACAGGGCTGAAGCCGACTATCTTGCCGATGAGT
TATCCAAGGCTTTGTTAGCTCACCATCACCATCACCACGGTGAACAAAAG
CTAATCTCCGAGGAAGACTTGTAAGCGGCCGC

Amino Acid Sequence

MSSVQLSRGDFHSIFTNKQRYDNPTGGVYQVYNTRKSDGANSNRKNLIMI
SDGIYHMKALLRNQAASKFQSMELQRGDIIRVIIAEPAIVRERKKYVLLV
DDFELVQSRADMVNQTSTFLDNYFSEHPNETLKDEDITDSGNVANQTNAS
NAGVPDMLHSNSNLNANERKFANENPNSQKTRPIFAIEQLSPYQNVWTIK
ARVSYKGEIKTWHNQRGDGKLFNVNFLDTSGEIRATAFNDFATKFNEILQ
EGKVYYVSKAKLQPAKPQFTNLTHPYELNLDRDTVIEECFDESNVPKTHF
NFIKLDAIQNQEVNSNVDVLGIIQTINPHFELTSRAGKKFDRRDITIVDD
SGFSISVGLWNQQALDFNLPEGSVAAIKGVRVTDFGGKSLSMGFSSTLIP
NPEIPEAYALKGWYDSKGRNANFITLKQEPGMGGQSAASLTKFIAQRITI
ARAQAENLGRSEKGDFFSVKAAISFLKVDNFAYPACSNENCNKKVLEQPD
GTWRCEKCDTNNARPNWRYILTISIIDETNQLWLTLFDDQAKQLLGVDAN
TLMSLKEEDPNEFTKITQSIQMNEYDFRIRAREDTYNDQSRIRYTVANLH
SLNYRAEADYLADELSKALLAHHHHHHGEQKLISEEDL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11554
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid.; Sequence difference compared with ref:; Extra c nucleotide after g387 in promoter sequence.