cDNA Clone slit-robo-GAP 2

• Plasmid: pAS2.6 slit-robo-GAP 2a
• Parent Plasmid: pAS2.6
• Expressed: GAL4-DNA-BD slit-robo-GAP 2 isoform a
• Genbank: NM_015326
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

gcggccgcgatgacgtctccagccaaattcaaaaaggataaggagatcatagcagagtacgatactcaggtcaaagagatccgtgctcagctcacagagcagatgaaatgcctggaccagcagtgtgagcttcgggtgcaactgttgcaggacctccaggacttcttccgaaagaaggcagagattgagatggactactcccgcaacctggagaagctggcagaacgcttcctggccaagacacgcagcaccaaggaccagcaattcaagaaggatcagaatgttctctctccagtcaactgctggaatctcctcttaaaccaggtgaagcgggaaagcagggaccataccaccctgagtgacatctacctgaataatatcattcctcgatttgtacaagtcagcgaggactcaggaagactctttaaaaagagtaaagaagtcggccagcagctccaa
gatgatttgatgaaggtcctgaacgagctctactcggtcatgaagacata
tcacatgtacaatgccgacagcatcagtgctcagagcaaactaaaggagg
cggagaagcaggaggagaagcaaattggtaaatcggtaaagcaggaggac
cggcagaccccacgctcccctgactccacggccaatgttcgcattgagga
gaaacatgtccggaggagctcagtgaagaagattgagaagatgaaggaga
agcgccaagccaagtacacggagaataagctgaaggccatcaaagcccgg
aatgagtacttgctggctttggaggcaaccaatgcatctgtcttcaagta
ctacatccatgacctatctgaccttattgatcagtgttgtgacttaggct
accatgcaagtctgaaccgggctctacgcaccttcctctctgctgagtta
aacctggaacagtcgaagcatgagggtctggatgccatcgagaatgcagt
agaaaacctggatgccaccagtgacaagcagcgcctcatggagatgtaca
acaacgtcttctgcccccctatgaagtttgagtttcagccccacatgggg
gatatggcttcccagctctgtgcccagcagcctgtccagagtgagctggt
acagagatgccaacaactgcagtctcgcttatccactctaaagattgaaa
acgaagaggtaaagaagacaatggaggccaccctgcaaaccatccaggac
attgtgactgtcgaggactttgatgtgtctgactgcttccagtacagcaa
ctccatggagtccgtcaagtccacggtctctgaaaccttcatgagcaagc
ccagcattgctaagaggagagccaaccagcaagagacagagcagttttat
ttcacaaaaatgaaagagtacctggagggcaggaacctcatcaccaagtt
acaagccaagcatgaccttctgcagaaaaccctgggagaaagtcagcgga
cagattgcagtctagccaggcgcagctcaactgtgaggaaacaggactcc
agccaggcaattcctctagtggtggaaagctgtatccggtttatcagcag
acacggactacagcatgaaggaattttccgggtgtcaggatcccaggtgg
aagtgaatgacatcaaaaatgcctttgagagaggagaggaccccctggct
ggggaccagaacgaccatgacatggattccatagctggtgtcctgaagct
ttacttccgggggctggaacaccctctcttccccaaggacatctttcatg
acctgatggcctgcgtcacaatggacaacctgcaggagagagctctgcac
atccggaaagtcctcctagtcctgcccaagaccactctgattatcatgag
atacctctttgccttcctcaatcatttatcacagttcagtgaagagaaca
tgatggacccctacaacctcgccatctgcttcgggccctcgctaatgtca
gtgccagagggccacgaccaggtgtcctgccaagcccacgtgaatgagct
gatcaaaaccatcatcatccagcatgagaacatcttcccaagccccaggg
agctggagggccctgtctacagcagaggaggaagcatggaggattactgt
gatagccctcatggagagactacctcggttgaagactcaacccaggatgt
gaccgcagagcaccacacgagcgatgacgaatgtgagcccatcgaggcca
ttgccaagtttgactacgtgggccggacagcccgagagctgtcctttaag
aagggagcatccctgctgctttaccagcgggcttccgacgactggtggga
aggccggcacaatggcatcgacggactcatcccccatcagtacatcgtgg
tccaagacaccgaggacggtgtcgtggagaggtccagccccaagtctgag
attgaggtcatttctgagccacctgaagaaaaggtgacagccagagcggg
ggccagctgtcccagtgggggtcatgtagccgatatttatcttgcaaaca
tcaacaagcaaaggaagcgtccagaatctgggagcatccggaaaactttt
cggagtgacagccacgggctgagcagttccctgactgactcctcctcccc
aggggtgggggctagctgccgcccatcctcccagcccatcatgagccaga
gcctccccaaagaagggccagataagtgttccatcagtgggcacgggagc
ctcaactccatcagccgccactcatccctgaagaatcggctggatagtcc
acagatccggaagactgccacagcgggaaggtcaaaaagcttcaataacc
atcggcccatggaccctgaggtcattgctcaggatattgaggcaacaatg
aactcggccctgaatgagctacgggaactagaacggcagagcagtgtcaa
acacacccctgacgtggttctggacaccttggagcccctcaaaacctccc
cagtggtggcccccacgtcagagccctccagccctctgcacacccagctc
ctcaaggaccccgagcccgccttccagcgcagcgccagtactgctgggga
catcgcctgcgccttccggcctgtcaagtctgtcaagatggctgccccgg
tcaaaccaccagccacacggcccaagcccactgtcttccccaaaacaaat
gccactagccctggtgtcaactcatcaacttccccacagtctactgacaa
gtcttgtactgtctgagcggccgc

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMGSPEFGRRAAAMTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLMACVTMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPSPRELEGPVYSRGGSMEDYCDSPHGETTSVEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSP
KSEIEVISEPPEEKVTARAGASCPSGGHVADIYLANINKQRKRPESGSIR
KTFRSDSHGLSSSLTDSSSPGVGASCRPSSQPIMSQSLPKEGPDKCSISG
HGSLNSISRHSSLKNRLDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIE
ATMNSALNELRELERQSSVKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLH
TQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFP
KTNATSPGVNSSTSPQSTDKSCTV*AA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11550
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015326 CDS: g489c, c636t, t705c, g1518a, a1830g, t2565c - all silent.