cDNA Clone slit-robo-GAP 3

• Plasmid: pAS2.6 slit-robo-GAP 3 isoform a
• Parent Plasmid: pAS2.6
• Expressed: GAL4-DNA-BD slit-robo-GAP 3 isoform a
• Genbank: NM_014850
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

gaattcatgtcatctcaaactaagttcaagaaagacaaagagatcattgctgaatatgaagcccaaataaaagagatccgcacgcagctggtggagcagttcaaatgtctggagcagcaatcagagtcgcgactgcagctgcttcaagacctccaggagtttttccgccggaaagctgagattgagctcgagtactcccgcagcctggagaagctggctgagcgcttctcctccaaaatccgcagctcccgggagcaccagttcaagaaggaccagtacctcctctcgcctgtgaactgttggtat
ctggttctgcatcagacccggcgggagagccgagaccatgccaccctcaa
tgacatcttcatgaacaatgtcatcgtccgcctctcccagatcagtgagg
atgtcatcagactcttcaaaaagagcaaggagattggcctgcagatgcac
gaggagctcctgaaggtgaccaatgagctctacacagtcatgaaaaccta
ccacatgtaccatgcagagagcatcagtgcggaaagcaagctgaaggagg
ctgagaagcaggaggagaagcagttcaataagtcaggagacctcagcatg
aacctgctccggcacgaggaccggccccagcgccgcagctctgtgaagaa
gattgagaagatgaaggagaagaggcaggccaagtactctgagaacaagc
tgaaatgcacaaaggcccggaatgactacttgctcaatctggcagccacc
aacgcagctataagcaaatactacatccatgatgtctctgatctgatcga
ttgctgtgatttgggcttccatgccagcctggcccgcaccttccggacct
atctctcagctgaatacaacctggagacctctcgccacgaagggctggat
gtcattgagaatgcagtggacaacctggattcccgaagtgacaagcacac
agtcatggacatgtgcaatcaagtcttctgccctccactcaagttcgagt
tccagccccacatgggggatgaggtctgccaggtcagcgctcagcagccc
gtccagacagaactgctcatgcgttatcaccagctgcagtccagactggc
caccctcaagatagagaatgaggaggttaggaaaaccctggatgccacca
tgcagacattacaggacatgctgactgtggaggactttgatgtctccgat
gccttccaacacagtcgatcgacagagtccgtcaagtcggctgcctctga
gacctacatgagcaagatcaacattgccaagaggagagccaaccagcagg
aaacagaaatgttttattttacaaaatttaaagagtatgtgaatggcagt
aacctcatcaccaagctgcaggccaagcacgatttactcaagcagaccct
gggcgaaggggaaagagcagaatgcggcaccaccaggcccccctgtcttc
cccctaaaccacagaaaatgaggagacctaggcctctctcagtgtatagc
cataaactctttaacggcagtatggaagcattcattaaggattcaggaca
agctataccgcttgtagtcgagagctgcatccgttacatcaatttatatg
gactccagcagcaggggatcttcagagtgccaggatctcaggtggaagtc
aatgacatcaaaaattcctttgagagaggtgaagacccccttgtggacga
tcaaaatgaacgagatatcaattcagtcgctggtgttttaaaactgtatt
tccgaggactggaaaacccactctttcctaaggaaaggtttcaagatttg
atatctactattaaactggagaacccagccgagagggtgcaccagatcca
acaaatcctcgtcacccttccccgcgtggtcattgtggtcatgagatacc
tcttcgctttcctcaaccacctctcccagtatagcgacgagaacatgatg
gatccctacaacctggccatctgcttcgggcctaccctcatgcacatccc
tgatgggcaggaccctgtgtcctgccaggcacacatcaatgaagtcatca
aaaccatcatcatccatcatgaagccatcttccccagcccccgggagcta
gagggacctgtgtatgaaaaatgcatggctggaggggaagaatattgtga
cagcccacacagcgagccaggggccatcgatgaagttgaccatgacaatg
gcactgagcctcataccagcgatgaagaagtggagcagatcgaggctatt
gccaagtttgactacatggggcggtccccgcgtgagctatccttcaagaa
gggggcctcgctgctcctgtaccaccgcgcctcggaggactggtgggagg
gccggcacaacggcgtggatggactcatcccccatcagtacatagttgta
caggacatggatgatgccttctccgacagcctgagccagaaggctgacag
cgaggccagcagtgggccattgctggatgacaaggcctcttccaaaaacg
acctccagtcccccacggagcacatctcggattacggctttgggggggtg
atgggccgagtgcggttacgatctgatggagcagccatccccagacgccg
aagcgggggcgacacacacagcccgccccggggcctgggccccagcatag
acacaccaccccgggctgctgcctgccccagcagcccccacaaaatcccc
ctcacccgggggaggatcgagagccctgagaagcggaggatggcgacgtt
cgggagcgctggcagcatcaactaccctgacaagaaggcgctctccgaag
ggcactcgatgaggtcgacctgcggttccaccaggcacagcagcctaggg
gaccacaagtccctggaggccgaggccctggcagaagacatcgagaagac
catgagcacggctctgcacgagttgcgggaactcgagaggcagaacacgg
tcaagcaggcgccagatgtggtgctggacaccctggagcccctgaagaac
ccgccaggccccgtcagctcggagcccgccagtccccttcacaccatcgt
catccgcgaccccgatgccgccatgcgccgcagcagcagctcctccaccg
agatgatgaccaccttcaagccagccctgtccgcccgcctggctggcgcc
cagctccgcccgccccccatgcggcccgtgcggccggtggtccagcaccg
gtccagcagcagcagcagctcgggcgtgggcagcccggccgtgacgccca
ccgagaagatgttccccaacagctcagcggacaagtcgggcaccatgtga
gcggccgc

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMGSPEFMSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLDVIENAVDNLDSRSDKHTVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESVKSAASETYMSKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKMRRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRYINLYGLQQQGIFRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPAERVHQIQQILVTLPRVVIVVMRYLFAFLNHLSQYSDENMM
DPYNLAICFGPTLMHIPDGQDPVSCQAHINEVIKTIIIHHEAIFPSPREL
EGPVYEKCMAGGEEYCDSPHSEPGAIDEVDHDNGTEPHTSDEEVEQIEAI
AKFDYMGRSPRELSFKKGASLLLYHRASEDWWEGRHNGVDGLIPHQYIVV
QDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTEHISDYGFGGV
MGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIP
LTRGRIESPEKRRMATFGSAGSINYPDKKALSEGHSMRSTCGSTRHSSLG
DHKSLEAEALAEDIEKTMSTALHELRELERQNTVKQAPDVVLDTLEPLKN
PPGPVSSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGA
QLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPNSSADKSGTM*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11549
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee