cDNA Clone Egr3

• Plasmid: pLZRSpBMN Egr3
• Parent Plasmid: pLZRS BMN LacZ
• Expressed: Egr3
• Genbank: NM_004430
• Species: Human

Sequence of Insert

AAGCTTATGACCGGCAAACTCGCCGAGAAGCTGCCGGTGACCATGAGCAG
TTTGCTAAACCAACTGCCTGACAATCTGTACCCCGAGGAGATCCCCAGCG
CGCTCAACCTCTTCTCCGGCAGCAGCGACTCGGTAGTCCATTACAATCAG
ATGGCTACAGAGAATGTAATGGACATCGGTCTGACCAACGAGAAGCCCAA
CCCGGAACTCTCTTACTCCGGCTCCTTCCAGCCAGCCCCCGGCAACAAGA
CCGTGACCTACTTGGGAAAGTTCGCCTTCGACTCCCCTTCCAACTGGTGC
CAGGACAACATCATTAGCCTCATGAGCGCCGGCATCTTGGGGGTGCCCCC
GGCTTCAGGGGCGCTCAGCACGCAGACGTCCACGGCCAGCATGGTGCAGC
CACCGCAGGGTGACGTGGAGGCCATGTATCCCGCGCTACCCCCCTACTCC
AACTGCGGCGACCTCTACTCAGAGCCCGTGTCTTTCCACGACCCCCAGGG
CAATCCCGGGCTCGCCTATTCCCCCCAGGATTACCAATCGGCCAAGCCGG
CGTTGGACAGCAATCTCTTCCCCATGATTCCTGACTACAACCTCTACCAC
CACCCCAACGACATGGGCTCCATTCCGGAGCACAAGCCCTTCCAGGGCAT
GGACCCCATCCGGGTCAACCCGCCCCCTATTACCCCTCTGGAGACCATCA
AGGCATTCAAAGACAAGCAGATCCACCCGGGCTTTGGCAGCCTGCCCCAG
CCGCCGCTCACCCTCAAGCCCATCCGGCCCCGCAAGTACCCCAACCGGCC
TAGCAAGACACCGCTCCACGAACGGCCCCACGCGTGCCCGGCCGAGGGCT
GCGACCGCCGTTTCAGCCGTTCGGACGAGCTGACCCGGCACCTGCGCATC
CACACGGGCCACAAGCCCTTCCAGTGCCGGATCTGCATGCGGAGCTTCAG
CCGCAGCGACCACCTCACCACTCACATCCGCACTCATACGGGCGAGAAGC
CCTTTGCCTGCGAGTTCTGCGGGCGCAAGTTTGCGCGCAGCGACGAGCGC
AAGCGCCACGCCAAGATCCACCTCAAGCAAAAGGAGAAGAAGGCGGAGAA
GGGCGGTGCACCCTCTGCATCCTCGGCGCCCCCCGTGTCGCTGGCCCCCG
TGGTCACCACCTGCGCCTGAGCGGCCGC

Amino Acid Sequence

MTGKLAEKLPVTMSSLLNQLPDNLYPEEIPSALNLFSGSSDSVVHYNQMA
TENVMDIGLTNEKPNPELSYSGSFQPAPGNKTVTYLGKFAFDSPSNWCQD
NIISLMSAGILGVPPASGALSTQTSTASMVQPPQGDVEAMYPALPPYSNC
GDLYSEPVSFHDPQGNPGLAYSPQDYQSAKPALDSNLFPMIPDYNLYHHP
NDMGSIPEHKPFQGMDPIRVNPPPITPLETIKAFKDKQIHPGFGSLPQPP
LTLKPIRPRKYPNRPSKTPLHERPHACPAEGCDRRFSRSDELTRHLRIHT
GHKPFQCRICMRSFSRSDHLTTHIRTHTGEKPFACEFCGRKFARSDERKR
HAKIHLKQKEKKAEKGGAPSASSAPPVSLAPVVTTCA*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11542
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Retroviral expression vector. Ex Doreen Cantrell’s lab.; No kozak for Egr3.; Intermediate – plan to clone GFP at HindIII site to generate pLZRSpBMN GFP-Egr3; ; Estimated MW of expressed protein : 42611 Daltons