cDNA Clone PSF3 (yeast)

• Plasmid: pRS415 Pro-PSF3-6His-cMyc
• Parent Plasmid: pRS415
• Genbank: DQ333173.1 GI:84313436
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCAAACGCAGCAATCTGGGCATGAGATGTTTGGATCTTACGAAGATCCATAGCCAAACCACTTAGGAGGCCGAACAGCCAACACCAATTGGACCAGCGAGCTATTTTCTTACCGGTAAGAACGGTTACAGGAATCACTTTCAAAATTCTTAAAAGATTGACTTGGTCTAACGACAAATATGCAGCAAAGAAGATGTTTTTCAAAACATTGCAGACTCTAACGACGTTGTCGCTGGCCAACTTGTTATCGTAGAATTTAGCAGCTGCCTGCAAGTGGTTTAAAGGCTTCAGAAACCTCAGAAA
TTTTCTGACTGTGGTAAATTGAGCTTGTAATTGCCTGGCCAAGAGAGATG
AGTTCTGTACTGCTAAAAATCTTGCTAAATACTGCAGTAATCTGAGAACC
TTTTCTCTGCCAGCTGAGCCATCTAGAAATTTGACGAATCTCGTCACGGA
GGGATGATATACCAGTGTATCACAGACCATATTGATTATACTATTACTTG
ATGAAGTCTTTGAAGTAGTAGATGGAGACAACGGTTTTTCAGTTGTTGGA
GTCACTTCCCTTTTATATCTATAAATGATATACCCATCGCTTCGGGTTCT
CCGAGATAAATGCCGACGCGTAGTGAAAATCGCAATGGAATTGATGCCAA
CTTTGTTCTTGGTATTATATTTTTACAATAGAAGGGCAAGCATAGATGGG
TTACTATGACATTGATGATGTCCTAGCAGATGGGACAGAGTTTCCTTGTA
AATTTCAATATGATATTCCTGGTCTTGGTTATTTGGAAAACAACCCAGGA
CGACCCATAACGAAAAACACTAAACTAAACCTGCCACTATGGTTAGCAAG
AATCCTTGCAATTGTGGGTGGTGACGAAGCCTTGGTAGATGAAGAACCTG
TACCTTTTGTGGAACTCCTGCCACCAGATATGTTTTCTACGAAAGTTATG
AACGCTATAAAGACCGATCCGGTGGCTCTGGACTTGCATTCCATAAATTC
GCATTTCTTCAGTCTAGCCATAAAATGGATAATGTTGTTTAGCGAAAAAG
AACTGGCCAATGTCGTTAGTGAGCTACTTTTGCAACGTGCCCAAGAACTT
AATCATCATGCTAGTAGTTTATCGATTGATCTCAATGCAGACTCGACCGG
AAAAAATTCAGCGAACACTAATATAGCAACTAGCACATTTTTACTGAAAC
TAGAAGAAATGGAGAAGGAGATATATAAAAAGTCTCATGAATCTTATAAG
GACACGAAAAGGTGGATGTTTAAAAAACACCATCACCATCACCACGGTGA
ACAAAAGCTAATCTCCGAGGAAGACTTGTAAGCGGCCGC

Amino Acid Sequence

MGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRPITKNTKLNLPLWL
ARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDLHSI
NSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADS
TGKNSANTNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKKHHHHHH
GEQKLISEEDL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11536
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with reference sequence:; g54a, g99c, c148t, a163g (in Pro), and delta aaagg just before ATG; g834a (S45N in PSF3 orf) - in all clones sequenced.